Protein adsorption on titanium surfaces and their effect on osteoblast attachment

Abstract

The objective of this study was to investigate the adsorption of albumin and fibronectin on titanium (Ti) surfaces and the effect of preadsorbed albumin and fibronectin on osteoblast attachment in vitro. Bovine serum albumin and bovine fibronectin were used in this study. Maximum adsorption of bovine serum albumin and fibronectin on Ti surfaces was observed to occur after 180-min incubation. In the presence of preadsorbed proteins, osteoblast attachment on Ti surfaces was observed to be enhanced compared to control Ti surfaces. However, cell attachment was affected by the types of protein adsorbed. Preadsorbed albumin was observed to have no significant effect on the amount of osteoblast cells attached. In comparison to control Ti surface and Ti surfaces preadsorbed with albumin, Ti surfaces preadsorbed with fibronectin for 15 min was observed to significantly increase osteoblast cell attachment, whereas Ti surfaces preadsorbed with fibronectin for 180 min did not affect cell attachment. In addition, cell morphology of the attached cells on protein preadsorbed Ti surfaces was not affected by the type of protein used in this study. It was concluded from this study that the concentration of fibronectin adsorbed on Ti surfaces was higher compared to albumin. In addition, it was also concluded that the concentration of fibronectin on Ti surfaces plays a role in governing cell attachment.

Titanate nanowire scaffolds decorated with anatase nanocrystals show good protein adsorption and low cell adhesion capacity

“…[23][24][25][26] Osteoblast attachment on titanium surfaces in the presence of preadsorbed proteins was observed to be enhanced compared with osteoblast attachment on control titanium surfaces without preadsorbed proteins. 27 However, ANs@TiNWs with high protein adsorption showed low MG63 cell adhesive capacity in our study, which may be due to the special nanostructure of the ANs@ TiNWs, with their larger specific surface area favoring protein adsorption but limited binding sites available for adhesion of MG63 cells.…”

Comparative assessment of structural and biological properties of biomimetically coated hydroxyapatite on alumina (α‐Al2O3) and titanium (Ti‐6Al‐4V) alloy substrates

“…The other characteristic peaks are shown along with the (211) plane. Except the two characteristic peaks of the Ti6Al-4V alloy substrate in the range of 2H ¼ [40][41][42][43][44][45][46][47][48][49][50] , no other peaks of the substrate were noticed exhibiting a dense coverage of HAp. In Figure 1(b), the XRD pattern comprises only the characteristic peaks of the Ti-6Al-4V alloy substrate in the range 2H ¼ .…”

Optical Detection of Protein Adsorption on Doped Titanium Surface

“…(Ball et al, 1996;Höök et al, 2002a;Huang et al, 2003;Imamura et al, 2008;Jones et al, 2000;Walivaara et al, 1994;Yang et al, 2003) These kind of biomaterial applications made of titanium are satisfactory products, because of their ability to adsorb certain proteins. After implantation, within a few seconds, the biomaterial surface becomes coated with a film of adsorbed proteins mediating the interaction between the implant and the body environment.…”