Protein adsorption studies on 'standard' polymeric materials

Abstract

Adsorption of fibrinogen from buffer as a single protein and from plasma to four materials has been studied. The two NIH-NHLBI primary reference standards, filler free polydimethylsiloxane and low density polyethylene, were used along with polyvinylcholoride and cellulose materials supplied by the IUPAC Working Party. The materials were examined in both film and tubing form, except for polydimethylsiloxane which was studied only in tubing form. Adsorption was measured at room temperature using 125I-labelled fibrinogen. The order of adsorbed amounts in the single protein experiments was found to be: cellulose < PVC < PE = PDMS. Apparent adsorption affinities are in the same order. In plasma, all surfaces except cellulose showed maxima in adsorption as a function of plasma concentration after 5 min contact. This is indicative of initial adsorption followed by displacement of fibrinogen (the Vroman effect). Cellulose showed very low adsorption of fibrinogen from plasma. The Vroman maxima were more pronounced on the tubing samples than on the films, and, as for the single protein experiments, adsorption was found to be less on tubing than on film samples. A tentative interpretation of the Vroman effect data suggests that the order of procoagulant activity of the materials may be: PDMS = PE < PVC < cellulose.

Development of Polydimethylsiloxane Substrates with Tunable Elastic Modulus to Study Cell Mechanobiology in Muscle and Nerve

“…The surface energy of a substrate can affect the types and amounts of proteins that are able to adhere to the surface, affecting cell adhesion and behavior [40], [41]. We used water contact angle measurements to determine whether the surface energy was constant for the different PDMS formulations (Fig.…”

Surface modified cellulose scaffolds for tissue engineering

“…However, as cellulose is a hydrophilic material with low nonspecific protein adsorption (which is why mammalian cells do not readily adsorb to cellulose surfaces) (Wu et al 2003;Zou et al 2001;Pelton 2009;Brash and Ten Hove 1993), these scaffolds required the addition of matrix ligands, to facilitate cell attachment to their surfaces (Singh et al 2013;Modulevsky et al 2014;Torres-Rendon et al 2015;Feldmann et al 2015). Watanabe et al (1993) demonstrated that, by introducing an ionic charge to cellulose membranes, collagen could be adsorbed to the membrane surface to promote cellular adhesion.…”

A comparative study of electrochemical techniques in investigating the adsorption behaviour of fibrinogen on platinum

“…Guemouri et al [58] also demonstrated that the adsorption of extracellular matrix protein fibronectin on a Si 0.78 Ti 0.22 O 2 surface is a reversible process. The Langmuir isotherm, which has often been successfully applied to describe the adsorption of proteins onto solid surfaces [59][60][61][62], can be described by:…”