Protein Corona Formation on Magnetic Nanoparticles Conjugated with Luminescent Europium Complexes

Abstract

The evaluation of the nanoparticles interactions with blood plasma enables a first and important insight on the organization of the adsorbed protein layer. In this context, we studied the formation of protein corona on magneto-luminescent Fe 3 O 4 @calix-Eu(TTA) nanoparticles in human plasma. The difference in the surface chemistry of F 3 O 4 functionalized with calixarene (+ 30 mV z-potential) and europium (III) thenoyltrifluoroacetonate (Eu 3 + -TTA) complex (+ 7.4 mV z-potential) affected the colloidal stability and hard corona composition of the nanoparticles, which were monitored by SDS-PAGE gel electrophoresis, differential centrifugal sedimentation analyses and luminescence spectroscopy. Strikingly, after conjugation with Eu 3 + TTA complex, the magnetic nanoparticles show lower adsorption affinity to proteins at higher blood plasma concentration. Moreover, the Eu 3 + compound is featured with the narrow emission lines of 5 D 0 ! 7 F J (J = 0-4) transitions, imparting additional characteristics to bifunctional nanoparticles that were used as probes to evaluate the protein corona formation.[a] Dr.

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