Protein expression profiling during wallerian degeneration after rat sciatic nerve injury

Li, Meiyuan; Zhang, Pingan; Guo, Weimin; Li, Huaiqin; Gu, Xiaosong; Yao, Dengbing

doi:10.1002/mus.24082

Cited by 25 publications

(16 citation statements)

References 40 publications

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“…Peripheral nerve injuries (PNI) caus e serious health problems, and there is no easily available formula for successful treatment 1 , 21 . After PNI, a series of signal cascades can be triggered, accompanied with the alteration of various gene expressions 3 .…”

Section: Discussionmentioning

confidence: 99%

“…Peripheral nerve injury (PNI), one of serious health problems, can often lead to lifelong disability 1 . Despite many preclinical and clinical studies have made significant progress in understanding the mechanism underlying this disease 2 , many complicated pathophysiology processes will happen, including a series of cellular and molecular responses accompanied with the alteration of various gene expressions after PNI 3 .…”

Section: Introductionmentioning

confidence: 99%

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Analysis of temporal expression profiles after sciatic nerve injury by bioinformatic method

Zhang

Zhan

Han

et al. 2017

Sci Rep

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After Peripheral nerve injuries (PNI), many complicated pathophysiologic processes will happen. A global view of functional changes following PNI is essential for the looking for the adequate therapeutic approaches. In this study, we performed an in-depth analysis on the temporal expression profiles after sciatic nerve injury by bioinformatic methods, including (1) cluster analysis of the samples; (2) identification of gene co-expression modules(CEMs) correlated with the time points; (3) analysis of differentially expressed genes at each time point (DEGs-ET); (4) analysis of differentially expressed genes varying over time (DEGs-OT); (5) creating Pairwise Correlation Plot for the samples; (6) Time Series Regression Analysis; (7) Determining the pathway, GO (gene ontology) and drug by enrichment analysis. We found that at a 3 h “window period” some specific gene expression may exist after PNI, and responses to lipopolysaccharide (LPS) and TNF signaling pathway may play important roles, suggesting that the inflammatory microenvironment exists after PNI. We also found that troglitazone was closely associated with the change of gene expression after PNI. Therefore, the further evaluation of the precise mechanism of troglitazone on PNI is needed and it may contribute to the development of new drugs for patients with PNI.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of temporal expression profiles after sciatic nerve injury by bioinformatic method

Zhang

Zhan

Han

et al. 2017

Sci Rep

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“…The first event occurring after a peripheral nerve injury is a series of cellular and molecular events called Wallerian degeneration, and was described for the first time by August Waller in 1850 . During this step, the myelin sheath is degraded and several axon ends are sealed, resulting in the disintegration of neurofilaments and microtubes . Meanwhile, the metabolism of proteins is changed, resulting in the increased synthesis of regenerative materials and Schwann cells.…”

Section: Nervous System Regenerationmentioning

confidence: 99%

Peripheral Nerve Regeneration: Current Status and New Strategies Using Polymeric Materials

Pinho

Fonseca

Serra

et al. 2016

Adv Healthcare Materials

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Experiments concerning peripheral nerve regeneration have been reported since the end of the 19th century. The need to implement an effective surgical procedure in terms of functional recovery has resulted in the appearance of several approaches to solve this problem. Nerve autograft was the first approach studied and is still considered the gold standard. Since autografts require donor harvesting, other strategies involving the use of natural materials have also been studied. Nevertheless, the results were not very encouraging and attention has moved towards the use of nerve conduits made from polymers, whose properties can be easily tailored and which allow the nerve conduit to be easily processed into a variety of shapes and forms. Some of these materials are already approved by the US Food and Drug Administration (FDA), as is presented here. Furthermore, polymers with conductive properties have very recently been subject to intensive study in this field, since it is believed that such properties have a positive influence in the regeneration of the new axons. This manuscript intends to give a global view of the mechanisms involved in peripheral nerve regeneration and the main strategies used to recover motor and sensorial function of injured nerves.

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“…Recently, microarray techniques have been widely used to examine the expression changes of thousands of genes and proteins during peripheral nerve regeneration, and a series of key elements, pathways, and biological processes have been identified [ 13 – 15 ]. The obtained findings largely expand the understanding of molecular mechanisms that are responsible for peripheral nerve regeneration.…”

Section: Introductionmentioning

confidence: 99%

Deep Sequencing and Bioinformatic Analysis of Lesioned Sciatic Nerves after Crush Injury

et al. 2015

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The peripheral nerve system has an intrinsic regenerative capacity in response to traumatic injury. To better understand the molecular events occurring after peripheral nerve injury, in the current study, a rat model of sciatic nerve crush injury was used. Injured nerves harvested at 0, 1, 4, 7, and 14 days post injury were subjected to deep RNA sequencing for examining global gene expression changes. According to the temporally differential expression patterns of a huge number of genes, 3 distinct phases were defined within the post-injury period of 14 days: the acute, sub-acute, and post-acute stages. Each stage showed its own characteristics of gene expression, which were associated with different categories of diseases and biological functions and canonical pathways. Ingenuity pathway analysis revealed that genes involved in inflammation and immune response were significantly up-regulated in the acute phase, and genes involved in cellular movement, development, and morphology were up-regulated in the sub-acute stage, while the up-regulated genes in the post-acute phase were mainly involved in lipid metabolism, cytoskeleton reorganization, and nerve regeneration. All the data obtained in the current study may help to elucidate the molecular mechanisms underlying peripheral nerve regeneration from the perspective of gene regulation, and to identify potential therapeutic targets for the treatment of peripheral nerve injury.

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Protein expression profiling during wallerian degeneration after rat sciatic nerve injury

Abstract: These results provide information related to functional analysis of differentially expressed genes during WD.

Cited by 25 publications

References 40 publications

Analysis of temporal expression profiles after sciatic nerve injury by bioinformatic method

Analysis of temporal expression profiles after sciatic nerve injury by bioinformatic method

Peripheral Nerve Regeneration: Current Status and New Strategies Using Polymeric Materials

Deep Sequencing and Bioinformatic Analysis of Lesioned Sciatic Nerves after Crush Injury

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