Protein Free Culture of R-Cho and Hybridoma Cells on the Macroporous Polypore® Carrier

Blüml, G.; Reiter, Manfred; Zach, N.; Gaida, T.; Schmatz, C.; Assadian, A.; Strutzenberger, K.; Katinger, Hermann

doi:10.1016/b978-0-7506-1845-8.50065-x

Cited by 4 publications

(2 citation statements)

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“…However, the serum-free medium formulation used in this study was not published. In addition, many formulations for recombinant CHO cells expressing a variety of proteins are described (Asakura et al, 1992;Blüml et al, 1994;Hata et al, 1992;Ii et al, 1995;Kim et al, 1998;Kitano, 1994;Lee et al, 1999;Matthews et al, 1994;Mizuguchi et al, 1993;Scharfenberg and Wagner, 1995;Schorn et al, 1994;Wyatt, 1994). However, a versatile serum-free medium formulation for the parental cell line DUKXB11 has not been reported to date.…”

Section: Introductionmentioning

confidence: 99%

Serum- and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11

Schröder

Matischak

Friedl

2004

Journal of Biotechnology

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Citation for published item:hr¤ oderD wF nd w tis h kD uF nd priedlD F @PHHRA 9 erumE nd proteinEfree medi formul tions for the ghinese h mster ov ry ell line h u fIIF9D tourn l of iote hnologyFD IHV @QAF ppF PUWEPWPF Further information on publisher's website: httpXGGdxFdoiForgGIHFIHITGjFj iote FPHHQFIPFHHS Publisher's copyright statement:Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-pro t purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. AbstractThe production of therapeutic proteins in mammalian cell lines is of outstanding importance. The maintenance of most mammalian cell lines in culture requires the addition of serum to the culture medium.The elimination of serum from mammalian cell culture is desirable since serum is expensive and a source of contaminants, e.g. viruses, mycoplasma or prions. Here we describe the composition of serum-and protein-free media for the Chinese hamster ovary (CHO) cell line DUKXB11. The serum-free formulation supports excellent growth of CHO DUKXB11 cells at low (23 cells/cm 2 ) and high (2·10 4 cells/cm 2 ) seeding densities characterized by a generation time of 10 -12 h, and, after addition of 0.2% pluronic F-68, the growth of a recombinant suspension cell line derived from DUKXB11. In addition, this formulation also allowed us to adapt recombinant cell lines expressing various amounts of human antithrombin ATIII (ATIII)to serum-free conditions. Secretion of ATIII was readily observed in the serum-free medium. Minor changes to the serum-free formulation resulted in a protein free formulation that supported growth of CHO DUKXB11 cells, growth of recombinant CHO cells expressing ATIII, and production of ATIII. Key wordsChinese hamster ovary cells, serum-free medium, protein-free medium, recombinant protein production,

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Section: Introductionmentioning

confidence: 99%

Serum- and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11

Schröder

Matischak

Friedl

2004

Journal of Biotechnology

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“…Some genetically modified (and hence less demanding) cell cultures like genetically tailored CHO-cells have been successfully employed in larger production scales [5,7], but none of them have ever scaled up primary cell-cultures to this extent.…”

Section: Huge Scale Bioprocessesmentioning

confidence: 99%

Single-cell-bioreactors as end of miniaturization approaches in biotechnology: progresses with characterised bioreactors and a glance into the future

Meier

Huebner

2005

Bioprocess Biosyst Eng

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Incidents with single cells and their genesis have not been the major focus of science up to now. This fact is supported by the difficulties one faces when wanting to monitor and cultivate small populations of cells in a defined compartment under controlled conditions, in vitro. Several approaches of up- and down-scaling have often led to poorly understood results which might be better elucidated by understanding the cellular genesis as a function of its microenvironment. This review of the approaches of scale-up and scale-down processes illustrates technical possibilities and shows up their limitations with regard to obtainable data for the characterisation of cellular genesis and impact of the cellular microenvironment. For example, stem cell research advances underline the lack of information about the impact of the microenvironment on cellular development. Finally, a proposal of future research efforts is given on how to overcome this lack of data via a novel bioreactor setup.

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The Viable Cell Monitor: A Dielectric spectroscope for Growth and Metabolic Studies of Animal Cells on Macroporous Beads

Guan

Кемп

New Developments and New Applications in Animal Cell Technology

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Protein Free Culture of R-Cho and Hybridoma Cells on the Macroporous Polypore® Carrier

Cited by 4 publications

References 4 publications

Serum- and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11

Serum- and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11

Single-cell-bioreactors as end of miniaturization approaches in biotechnology: progresses with characterised bioreactors and a glance into the future

The Viable Cell Monitor: A Dielectric spectroscope for Growth and Metabolic Studies of Animal Cells on Macroporous Beads

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