Protein glycation inhibitory activities ofLawsonia inermisand its active principles

Abstract

The protein glycation inhibitory activity of ethanolic extract of Lawsonia inermis (henna) plant tissues was evaluated in vitro using the model system of bovine serum albumin and glucose. Protein oxidation and glycation are posttranslational modifications that are implicated in the pathological development of many age-related disease processes. This study investigated the effects of Lawsonia inermis ethanolic extract and its components, on protein damage induced by a free radical generator in in vitro assay system. We found that alcoholic extract of Lawsonia inermis can effectively protect against protein damage and showed that its action is mainly due to Lawsone. In addition, the presence of gallic acid also plays an important role in the protective activity against protein oxidation and glycation. Two known compounds, namely, Lawsone and gallic acid previously isolated from this plant were subjected to glycation bioassay for the first time. It was found that the alcoholic extract, lawsone (1) and gallic acid (2) showed significant inhibition of Advanced Glycated End Products (AGEs) formation and exhibit 77.95%, 79.10% and 66.98% inhibition at a concentration of 1500 mg/mL, 1000 mg/mL and 1000 mM respectively. Lawsonia inermis, compounds 1 and 2 were found to be glycation inhibitors with IC 50 82.06^0.13 mg/mL, 67.42^1.46 mM and 401.7^6. 23 mM respectively. This is the first report on the glycation activity of these compounds and alcoholic extract of Lawsonia inermis.

Comparison between the effect of Lawsonia inermis and flubendazole on Strongyloides species using scanning electron microscopy

“…On the other hand, the methanolic extract was effective probably due to the involvement of alkaloids, glycosides, terpenoids and flavonoids. Previously, also Sultana et al (2009) demonstrated that ethanol extract and its components lawsone and gallic acid showed significant protein glycation inhibitory activity.…”

Design for carbon–carbon bond forming reactions under ambient conditions

“…Scheme 28. [198][199][200] This phenolic quinone compound has been evaluated to possess a wide range of biological and pharmacological activities such as antioxidant, 201 antibacterial, 202 antifungal, 203 cytotoxic, 204 trypsin inhibitor, 205 anticoagulant, 206 advanced glycated end products (AGEs) formation inhibitor, 207 and anti-acute pancreatitis. Proposed mechanism for the sulfamic acid-catalyzed synthesis of 3,3-bis(indol-3yl)indolin-2-ones (77) 6-Aminouracil and its derivatives play a key structural part in numerous natural and synthetic bioactive compounds, and also regarded as a versatile building block for several nitrogen-containing heterocycles possessing a wide range of pharmacological potentials.…”

Design of Organic Transformations at Ambient Conditions: Our Sincere Efforts to the Cause of Green Chemistry Practice

“…(synonyms: L. alba , L. spinosa ; also known as henna or mhendi ; family: Lythraceae), different parts of which have been traditionally used all over the world as cosmetics (hair dye, body paint and tattoo dye) and herbal remedies in treating various ailments . This phenolic quinone compound has been evaluated to possess a wide range of biological and pharmacological activities such as antioxidant, antibacterial, antifungal, cytotoxic, trypsin inhibitor, anticoagulant, advanced glycation end products (AGEs) formation inhibitor, and anti‐acute pancreatitis . The interesting structural pattern of lawsone coupled with its so‐called multifaceted pharmacological potential has motivated chemists to explore this useful scaffold in certain chemical processes, particularly in synthesizing ligands for metal complexation, and a few of its derivatives have already been reported to exhibit a number of biological activities …”