Protein Inhibitors of -amylase in Mature and Germinating Grain of Rye ( )

Täufel, A.; Böhm, H.; Flamme, Wilhelm

doi:10.1006/jcrs.1996.0093

Cited by 7 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Marshall and Lauda () proposed that two α‐amylase inhibitors in wheat flour can inhibit insect and mammalian amylase activity to varying degrees (Carbonaro et al ). The α‐amylase inhibitors in soybean (Tufel et al ) and in microbes (Sørensen et al ) have been studied extensively (Kataoka and DiMagno ). Transgenic cowpeas that express the α‐amylase inhibitor‐1 of bean ( Phaseolus vulgaris ) strongly inhibited the development of the bruchid beetles Callosobruchus maculatus and Callosobruchus chinensis in insect bioassays (Solleti et al ).…”

Section: Introductionmentioning

confidence: 99%

A novel wheat α‐amylase inhibitor gene, TaHPS, significantly improves the salt and drought tolerance of transgenic Arabidopsis

Xiao

Huang

Shen

et al. 2012

Physiologia Plantarum

View full text Add to dashboard Cite

On the basis of microarray analyses of the salt-tolerant wheat mutant RH8706-49, a previously unreported salt-induced gene, designated as TaHPS [Triticum aestivum hypothetical (HPS)-like protein], was cloned. Real-time quantitative polymerase chain reaction analyses showed that expression of the gene was induced by abscisic acid, salt and drought. The encoded protein was found to be localized mainly in the plasma membranes. Transgenic Arabidopsis plants overexpressing TaHPS were more tolerant to salt and drought stresses than non-transgenic wild-type (WT) plants. Under salt stress, the root cells of the transgenic plants secreted more Na⁺ and guard cells took up more Ca²⁺ ions. Compared with wild-type plants, TaHPS-expressing transgenic plants showed significantly lower amylase activity and glucose and malic acid levels. Our results showed that the expression of TaHPS inhibited amylase activity, which subsequently led to a closure of stomatal apertures and thus improved plant tolerance to salt and drought.

show abstract

Section: Introductionmentioning

confidence: 99%

A novel wheat α‐amylase inhibitor gene, TaHPS, significantly improves the salt and drought tolerance of transgenic Arabidopsis

Xiao

Huang

Shen

et al. 2012

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…One of these EST-contigs showed similarity with the trypsin/ alpha-amylase inhibitor CMx1/CMx3, which is known to inhibit the production of germination specific alpha-amylase, thus regulating the amount of sugars released from starchy endosperm during germination, which not only helps in preventing precocious germination (i.e. pre-harvest sprouting) but also protects seeds from predators (Taufel et al 1997). Interestingly, the map locations of CMx (CMx1, CMx2 and CMx3) genes also coincide well with the known location of three major dormancy QTLs detected on group 4 chromosomes of bread wheat (Sanchez De La Hoz et al 1994;Mori et al 2005), thus suggesting the usefulness of these SNP markers in gene-assisted selection (GAS) for pre-harvest sprouting tolerance (PHST).…”

Section: Annotation Of Est-contigsmentioning

confidence: 99%

EST-SNPs in bread wheat: discovery, validation, genotyping and haplotype structure

Rustgi¹,

Bandopadhyay²,

Balyan³

et al. 2009

CZECH J GENET PLANT

View full text Add to dashboard Cite

Abstract:The present study involves discovery, validation and use of single-nucleotide polymorphisms (SNPs) in bread wheat utilizing 48 EST-contigs (individual contigs having 20-89 ESTs, derived from 2 to 11 different genotypes). In order to avoid a problem due to homoeologous relationships, the ESTs in each contig were classified into 175 sub-contigs (3.7 sub-contigs/EST-contig) using characteristic homoeologue sequence variants (HSVs), which had a density of 1 HSV every 136.7 bp. In silico analysis of sub-contigs led to the discovery of 230 candidate EST-SNPs with a density of 1SNP/273.9 bp. Locus specific primers (each primer pair flanking 1-18 SNPs) were designed utilizing one sub-contig each from 42 EST-contigs that contained SNPs, the remaining 6 contigs having no SNPs. To provide locus specificity to the PCR products, each primer was tagged with an HSV at its 3' end. Only 10 primer pairs, which gave each a characteristic solitary band, were utilized to validate EST-SNPs over 30 diverse bread wheat genotypes; 7 SNPs were validated through resequencing the PCR products. Allele specific primers were designed and utilized for genotyping of 50 diverse bread wheat accessions (including 30 bread wheat genotypes previously used for validation of SNPs), with an aim to test their utility in genotyping and map construction. The allele specific primers allowed the classification of 50 genotypes in two alternative allele groups for each SNP as expected, thus suggesting their utility for genotyping. Of the above 7 validated SNPs, 4 belonged to a solitary locus (PKS37); 7 haplotypes were available at this locus. Altogether, the results suggested that EST-SNPs constitute an important source of molecular markers for studies on wheat genomics.

show abstract