Protein Kinase Assay on Peptide‐Conjugated Gold Nanoparticles by Using Secondary‐Ion Mass Spectrometric Imaging

Kim, Young‐Pil; Oh, Eunkeu; Oh, Youjin; Moon, Dae Won; Lee, Tae Geol; Kim, Kwang Ho

doi:10.1002/ange.200701418

Cited by 25 publications

(20 citation statements)

References 26 publications

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“…The high sensitivity of TOF-SIMS for some functional groups has been usefully applied in many ways. For example, it has been used to examine peptides conjugated to gold nanoparticles as part of a protein kinase assay [52] and to examine multilayer plasma deposited organic coatings on alumina nanoparticles [53]. For relatively large nanoparticles produced during welding, SIMS with sputter profiling has been used to examine the complex layers that forms on these particles [47].…”

Section: Ion-based Surface Analysis Methodsmentioning

confidence: 99%

Application of surface chemical analysis tools for characterization of nanoparticles

et al. 2010

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The important role that surface chemical analysis methods can and should play in the characterization of nanoparticles is described. The types of information that can be obtained from analysis of nanoparticles using Auger electron spectroscopy (AES); X-ray photoelectron spectroscopy (XPS); time of flight secondary ion mass spectrometry (TOF-SIMS); low energy ion scattering (LEIS); and scanning probe microscopy (SPM), including scanning tunneling microscopy (STM) and atomic force microscopy (AFM), are briefly summarized. Examples describing the characterization of engineered nanoparticles are provided. Specific analysis considerations and issues associated with using surface analysis methods for the characterization of nanoparticles are discussed and summarized, along with the impact that shape instability, environmentally induced changes, deliberate and accidental coating, etc., have on nanoparticle properties.

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Section: Ion-based Surface Analysis Methodsmentioning

confidence: 99%

Application of surface chemical analysis tools for characterization of nanoparticles

et al. 2010

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“…51,52 Among these methods, colorimetric assays based on the aggregation or redispersion of gold nanoparticles (AuNPs) are in particular prevalent because of their simple manipulation principle and easy detection procedure. Usually, there are two categories of the colorimetric sensors based on the AuNPs used: one is based on the cross-linking aggregation of AuNPs functionalized respectively with recognition elements (eg, avidin and antibody) and kinase-specific peptide, 10,11,14,15 and the other is based on the non-cross-linking aggregation of unmodified AuNPs, which is tuned by the phosphorylation-induced net charge change of substrate peptide.…”

mentioning

confidence: 99%

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Sun¹,

Chang²,

Zhou³

et al. 2017

IJN

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This article presents a general method for the detection of protein kinase with a peptide-like kinase inhibitor as the bioreceptor, and it was done by converting gold nanoparticles (AuNPs)-based colorimetric assay into sensitive electrochemical analysis. In the colorimetric assay, the kinase-specific aptameric peptide triggered the aggregation of AuNPs in solution. However, the specific binding of peptide to the target protein (kinase) inhibited its ability to trigger the assembly of AuNPs. In the electrochemical analysis, peptides immobilized on a gold electrode and presented as solution triggered together the in situ formation of AuNPs-based network architecture on the electrode surface. Nevertheless, the formation of peptide–kinase complex on the electrode surface made the peptide-triggered AuNPs assembly difficult. Electrochemical impedance spectroscopy was used to measure the change in surface property in the binding events. When a ferrocene-labeled peptide (Fc-peptide) was used in this design, the network of AuNPs/Fc-peptide produced a good voltammetric signal. The competitive assay allowed for the detection of protein kinase A with a detection limit of 20 mU/mL. This work should be valuable for designing novel optical or electronic biosensors and likely lead to many detection applications.

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“…Kim developed a label-free assay of protein kinase on peptide conjugated AuN�s by using secondary-ion mass spectrometer, where the AuN�s acted as both signal enhancers and target concentrators [85]. Two Cys-terminated peptides with different lengths were conjugated to AuN�s based on the affinity between Au and thiol groups.…”

Section: Naldi For Ms-based Biomedical Analysis Technology Reportmentioning

confidence: 99%

Nanomaterial-Assisted Laser Desorption Ionization for Mass Spectrometry-Based Biomedical Analysis

Qiao

Liu

2010

Nanomedicine

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Nanomaterials have been widely used to assist laser desorption ionization of biomolecules for mass spectrometry analysis. Compared with classical matrix-assisted laser desorption ionization, strategies based on nanomaterial-assisted ionization generate a clean background, which is of great benefit for the qualitative and quantitative analysis of small biomolecules, such as therapeutic and diagnostic molecules. As label-free platforms, they have successfully been used for high-throughput enzyme activity/inhibition monitoring and also for tissue imaging to map in situ the distribution of peptides, metabolites and drugs. In addition to widely used porous silicon nanomaterials, gold nanoparticles can be easily chemically modified by thiol-containing compounds, opening novel interesting perspectives. Such functionalized nanoparticles have been used both as probes to extract target molecules and as matrices to assist laser desorption ionization for developing new enzyme immunoassays or for studying DNA hybridization. More recently, semiconductor nanomaterials or quantum dots acting as photosensitive centers to induce in-source redox reactions for proteomics and to investigate biomolecule oxidation for metabolomics have been shown to offer new analytical strategies.

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Protein Kinase Assay on Peptide‐Conjugated Gold Nanoparticles by Using Secondary‐Ion Mass Spectrometric Imaging

Cited by 25 publications

References 26 publications

Application of surface chemical analysis tools for characterization of nanoparticles

Application of surface chemical analysis tools for characterization of nanoparticles

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Nanomaterial-Assisted Laser Desorption Ionization for Mass Spectrometry-Based Biomedical Analysis

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