Protein Precipitation to Remove Carbohydrates that Interfere in Protein-Bound Tryptophan Quantification in Soybean Seeds

Lei, Zhentian; Kumar, Santosh; Sarma, Saurav J.; Kumar, Ritesh; Sumner, Barbara W.; Stacey, Minviluz G.; Sumner, Lloyd W.

doi:10.1007/s41664-020-00144-x

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…Proteins become positively charged when acid is added because the amino groups on the protein capture protons and the pH decreases. Therefore, precipitation results from an imbalance in the protein structure [14]. The addition of strong acids such as TCA can trigger strong protein-protein interactions, as opposed to protein-solution, resulting in precipitation [15].…”

Section: Results and Discussion Application Of Cap In Shrimpmentioning

confidence: 99%

“…The addition of strong acids such as TCA can trigger strong protein-protein interactions, as opposed to protein-solution, resulting in precipitation [15]. TCA 15% (w/v) is used because TCA concentration between 5%-45 % (w/v) is the most optimal for precipitating protein [14]. The photograph before and after addition of TCA shown in Figure 2.…”

Section: Results and Discussion Application Of Cap In Shrimpmentioning

confidence: 99%

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Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

Almira¹

2022

ijcsrr

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This research aimed to determine the concentration of chloramphenicol in shrimp using the standard addition method based on the diazotization reaction using Zn powder as a reducing agent of chloramphenicol, followed by the use of N-(1-naphthyl) ethylenediamine dihydrochloride as a coupling agent and measured at 565 nm. Based on the test, the shrimp sample was found to contain 1964.91 mg/kg of chloramphenicol and it exceeded the requirements set by the European Commission which was 0,15 mg/kg. The limit of detection (LOD) value is 0.19 mg/mL and the limit of quantitation (LOQ) value is 0.64 mg/mL. The correlation coefficient (R2) was 0.9991 for the concentration range of 0-50 ppm. The analysis of the results showed that the %recovery in shrimp analysis using this method was 87.41%-107.73% with an average of 109.38%.

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Section: Results and Discussion Application Of Cap In Shrimpmentioning

confidence: 99%

Section: Results and Discussion Application Of Cap In Shrimpmentioning

confidence: 99%

Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

Almira¹

2022

ijcsrr

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“…Based on the outcome of the sensory evaluation described in Section 2.3, rosemary extract was selected and evaluated individually for its total phenolic content (TPC), total sugar content, and total protein content. Various kinds of rosemary extract solutions with or without polyphenols, proteins, or polysaccharides were prepared according to previously reported methods (Carrasco‐Sanchez et al., 2017; Lei et al., 2020; Xu et al., 2014) with minor modifications. One hundred milligrams of rosemary extract was dissolved in 3 ml of ethanol, and then diluted to 100 ml with ultrapure water to obtain the T1 solution.…”

Section: Methodsmentioning

confidence: 99%

Deodorizing effects of rosemary extract on silver carp (Hypophthalmichthys molitrix) and determination of its deodorizing components

Huang

Wang

Shi

et al. 2022

Journal of Food Science

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Fishy odor in fish products severely influences both eating quality and commercial acceptability, and natural plant extracts, particularly spices, have recently become popular for the removal of fishy odor. This study aimed to explore the potential of rosemary extract for the deodorization of silver carp (Hypophthalmichthys molitrix), as well as to identify the deodorizing components in rosemary extract. Results showed that all of the spice extracts used in this study (ginger, garlic, angelica dahurica, fennel, rosemary, nutmeg, white cardamom, cinnamon, star anise, and bay leaf) significantly reduced the fishy odor value of silver carp, among which rosemary extract was most effective, decreasing the fishy odor value by about 58%. Gas chromatography‐mass spectrometry analysis and sensory evaluation showed that the fishy odor value and concentrations of the fishy odor‐active compounds were significantly reduced by the application of rosemary extract. However, the lower the total phenolic content of rosemary extract, the poorer the deodorizing effects against silver carp, suggesting that the deodorizing effect was primarily driven by polyphenols. Fourteen phenolic compounds were measured in rosemary extract, and three individual phenolic compounds (rosmarinic acid [RA], carnosic acid [CA], and carnosol [CS]) were chosen for deodorizing experiment. Sensory detection results and changes of contents of volatile showed that these three phenolic compounds are effective at removing the fishy odor. These results suggest that polyphenols are the main deodorizing components, and RA, CA, and CS are the main deodorizing active compounds in rosemary extract. Practical Application The results of this study may provide a new way to determine the deodorizing components of spice extracts. Moreover, it can provide guidance for further research in investigating the deodorizing mechanism of sipce extracts.

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“…The commonly used pretreatment method for determination of angoroside C in biological samples is protein precipitation [7,8]. Although protein precipitation is simple to operate, it requires a long time of centrifugation and nitrogen blowing, and the sensitivity and selectivity of the method cannot meet the demand for quantitative analysis of the extremely low content of angoroside C in plasma [10]. Therefore, more efficient pretreatment methods need to be explored.…”

Section: Introductionmentioning

confidence: 99%

Magnetic covalent‐organic frameworks‐based extraction followed by UHPLC‐MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule

Cao

Zhu

et al. 2022

J of Separation Science

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The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection. In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection. The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu⋅g -1 ), large surface area (110.9 m 2 ⋅g -1 ), and strong stability. The as-prepared material can quickly extract angoroside C from plasma with high extraction efficiency, be easily separated with a magnet afterward, and can be reused for at least five times. The established method was systematically validated showing good linearity (0.1-5 ng⋅ml -1 ), low limit of quantification (0.1 ng⋅ml -1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%). Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.

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Protein Precipitation to Remove Carbohydrates that Interfere in Protein-Bound Tryptophan Quantification in Soybean Seeds

Cited by 4 publications

References 20 publications

Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

Deodorizing effects of rosemary extract on silver carp (Hypophthalmichthys molitrix) and determination of its deodorizing components

Magnetic covalent‐organic frameworks‐based extraction followed by UHPLC‐MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule

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