Protein Profile and Hemagglutination Activity of Pilli, an Adhesion Factor Causing Typhoid Fever by Salmonella typhi

Darmawati, Sri; Ethica, Stalis Norma; Dewi, Sri Sinto

doi:10.1088/1755-1315/292/1/012049

Cited by 3 publications

(7 citation statements)

References 6 publications

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“…API 20E and API 50CHB /E medium (Bio Merieux Inc.) were utilized for identification of bacteria (Darmawati et al 2012). Bacterial cultures were cultured in biphasic medium (BHI Agar slant and BHI broth media) for 48 hours at 37ºC without agitation (Darmawati et al 2019). One bacterial colony on MC media was grown in 50 mL of liquid BHI as a starter, then the culture was incubated for 24 hours at 37ºC with agitation.…”

Section: Culture and Identification Of Salmonella Typhimentioning

confidence: 99%

“…and can stimulate an immune response. Adhesion to host cells is considered a key factor during bacterial pathogenesis (Darmawati et al 2015;Darmawati et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Hemagglutinin protein is a lectin, which can interact with carbohydrates on the surface of human erythrocytes specifically and is reversible, resulting in agglutination (clumping). Blood type A has N-Acetyl-D-Galactosamine carbohydrates, blood group B has D-Galactose, and blood group O has L-fucose (Darmawati et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Based on the results of an analysis of the pili protein conducted by Darmawati (2019) from isolates of S. typhi BA07.4 and S. typhi KD30.4, two main protein subunits measuring 87 and 42 kDa were observed on SDS-PAGE. Proteins that are immunogenic have a molecular weight of 10-100 kDa (Parslow et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Proteins that are immunogenic have a molecular weight of 10-100 kDa (Parslow et al 2001). Based on the hemaagglutination test, it was found that pilus proteins from S. typhi BA07.4 and S. typhi KD30.4 can agglutinate human erythrocytes in groups A, B, AB and O because they are able to identify receptors on the surface of erythrocytes (Darmawati et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of a 42 kDa subunit pili protein of Salmonella typhi causes typhoid fever

et al. 2022

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Abstract. Darmawati S, Ethica SN, Prastiyanto ME, Depamede SN, Putri EO, Kamaruddin M. 2022. Molecular characterization of a 42 kDa subunit pili protein of Salmonella typhi causes typhoid fever. Biodiversitas 23: 962-968. Blood culture is the gold standard for diagnosing typhoid fever, but it has limitations such as media and laboratory equipment, specimen volume, and examination time. However, the Academy of Pediatrics does not recommend serology due to its low sensitivity.The purpose of this study was to determine the molecular properties of the protein pilli of Salmonella typhi(S. typhi) that the findings can be used to develop a typhoid fever diagnostic reagent. The SDS-PAGE method was used, as well sequence analysis with ProtParam, ProtScale, and PSIPRED. The SDS-PAGE profile reveals one major protein (42 kDa) and fourteen minor proteins. The pili protein subunit 42 kDa had an amino acid (AA) sequence with a length of 390 AA, according to bioinformatics analysis. According to the ProtParam results, the pili protein subunit 42 kDa has good stability with a value of 40 and is a hydrophilic protein with an average GRAVY value of -0.950. PSIPRED results show that among the secondary structural elements, coil strand predominates, followed by -helix and -strand. It is concluded that this protein is immunogenic and that it can be used to develop a more specific and sensitive diagnostic reagent for typhoid fever.

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Section: Culture and Identification Of Salmonella Typhimentioning

confidence: 99%

“…and can stimulate an immune response. Adhesion to host cells is considered a key factor during bacterial pathogenesis (Darmawati et al 2015;Darmawati et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of a 42 kDa subunit pili protein of Salmonella typhi causes typhoid fever

et al. 2022

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The pili at genomic level

Farnia,

Ghanavi,

Aghajani

et al. 2024

Pili in Mycobacterium Tuberculosis

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Effects of cinnamon essential oil on the physiological metabolism of Salmonella enteritidis

et al. 2022

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Food safety and health are the themes of today's society. As a class of foodborne pathogens, Salmonella enteritidis has become one of the common zoonotic pathogens. Because chemical preservatives have certain harmfulness and have been questioned, it is particularly important to find green and safe natural preservatives. The advantages of plant essential oils (EOs) are that they are green and safe, have a wide range of antibacterials, and are not easy to form drug resistance. In recent years, studies have found that EOs have excellent antibacterial activity, but their antibacterial mechanism has not been conclusive, which has certain limitations in their application in the food field. Cinnamon essential oil (CEO) extracted from dried cinnamon is a secondary metabolite of cells and a very important natural food flavor. More importantly, it is non-toxic to the human body and has been proven to have a good antibacterial effect, but its antibacterial mechanism is still unclear. Therefore, it was of great practical significance to carry out the research on the antibacterial mechanism of CEO on S. enteritidis. In this work, S. enteritidis was used as the test bacteria, and CEO was selected as the antibacterial agent to study the antibacterial mechanisms. By studying the physiological metabolism of S. enteritidis cells by CEO, the influence of CEO on the bacteriostatic mechanism of S. enteritidis was systematically elucidated. The study found that CEO treatment would reduce the activity of bacterial metabolism. It is mainly reflected in the following three aspects: first, the activity of key enzymes in TCA circulation is inhibited, thus affecting the respiration of S. enteritidis. Second, it affects the level of energy metabolism by inhibiting the content of adenosine triphosphate (ATP) and the activity of ATPase. Finally, it can affect the physiological metabolism of bacteria by inhibiting the metabolism of proteins and other substances. Therefore, this article was expected to provide a theoretical basis for the development of new natural food preservatives and the prevention and control of S. enteritidis.

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Protein Profile and Hemagglutination Activity of Pilli, an Adhesion Factor Causing Typhoid Fever by Salmonella typhi

Cited by 3 publications

References 6 publications

Molecular characterization of a 42 kDa subunit pili protein of Salmonella typhi causes typhoid fever

Molecular characterization of a 42 kDa subunit pili protein of Salmonella typhi causes typhoid fever

The pili at genomic level

Effects of cinnamon essential oil on the physiological metabolism of Salmonella enteritidis

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