Protein quality control of cell stemness

Yan, Pengze; Ren, Jie; Zhang, Weiqi; Qu, Jing; Liu, Guanghui

doi:10.1186/s13619-020-00064-2

Cited by 29 publications

(23 citation statements)

References 183 publications

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“…This mismatch has been described in many cell types, including stem cells ( Lu et al, 2009 ; Ingolia et al, 2011 ; Baser et al, 2019 ; Habowski et al, 2020 ; Spevak et al, 2020 ), suggesting that post-transcriptional control of gene expression is common. The protein content of a cell depends on both synthesis and degradation: work describing extensive links between protein degradation and stem cell fate has been reviewed elsewhere ( Strikoudis et al, 2014 ; Suresh et al, 2016 ; Yan et al, 2020 ); here, we will focus on the mechanisms affecting stem cell fate through the regulation of protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

mRNA Translation Is Dynamically Regulated to Instruct Stem Cell Fate

Wang

Amoyel

2022

Front. Mol. Biosci.

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Stem cells preserve tissue homeostasis by replacing the cells lost through damage or natural turnover. Thus, stem cells and their daughters can adopt two identities, characterized by different programs of gene expression and metabolic activity. The composition and regulation of these programs have been extensively studied, particularly by identifying transcription factor networks that define cellular identity and the epigenetic changes that underlie the progressive restriction in gene expression potential. However, there is increasing evidence that post-transcriptional mechanisms influence gene expression in stem cells and their progeny, in particular through the control of mRNA translation. Here, we review the described roles of translational regulation in controlling all aspects of stem cell biology, from the decision to enter or exit quiescence to maintaining self-renewal and promoting differentiation. We focus on mechanisms controlling global translation rates in cells, mTOR signaling, eIF2ɑ phosphorylation, and ribosome biogenesis and how they allow stem cells to rapidly change their gene expression in response to tissue needs or environmental changes. These studies emphasize that translation acts as an additional layer of control in regulating gene expression in stem cells and that understanding this regulation is critical to gaining a full understanding of the mechanisms that underlie fate decisions in stem cells.

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Section: Introductionmentioning

confidence: 99%

mRNA Translation Is Dynamically Regulated to Instruct Stem Cell Fate

Wang

Amoyel

2022

Front. Mol. Biosci.

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“…There are two reasons for this. Stem cells, by virtue of their inherent biological traits, harbor elevated proteostasis modulation and elevated levels of heat shock proteins [ 8 , 10 ]. Additionally, PDSCs are exposed to physiological oxidative stress within placental ecosystem that accords differential stress ameliorating capacity to these stem cells [ 22 , 23 , 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…PDSCs, similar to other stem cells, require elevated protein synthesis emanating from their requirements to maintain stemness and differentiation potential [ 6 , 7 , 8 , 9 ]. The requirement for dynamic protein synthesis is compounded by their ability to sense and respond to varying conditions and stresses from different physiological and cell-habitat sources [ 7 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…The protein homeostasis (proteostasis) network in the cells coordinates the proteome balance by regulating all steps of protein life cycle: synthesis, folding, conformational maintenance, and degradation [ 6 , 9 , 11 , 12 ]. There potentially is a close connection between proteostasis and stem cell function, highlighting the presence of stem cell intrinsic proteostasis mechanisms and their tight coupling to cellular properties and functions [ 7 , 8 , 13 , 14 ]. The determining goal for proteostasis is to ensure the operational levels of proteins in their native conformations, while simultaneously reducing the presence of deleterious products such as aggregates [ 6 , 12 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Heat-Induced Proteotoxic Stress Response in Placenta-Derived Stem Cells (PDSCs) Is Mediated through HSPA1A and HSPA1B with a Potential Higher Role for HSPA1B

Alharbi

Bugshan

Almozel

et al. 2022

CIMB

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Placenta-derived stem cells (PDSCs), due to unique traits such as mesenchymal and embryonic characteristics and the absence of ethical constraints, are in a clinically and therapeutically advantageous position. To aid in stemness maintenance, counter pathophysiological stresses, and withstand post-differentiation challenges, stem cells require elevated protein synthesis and consequently augmented proteostasis. Stem cells exhibit source-specific proteostasis traits, making it imperative to study them individually from different sources. These studies have implications for understanding stem cell biology and exploitation in the augmentation of therapeutic applications. Here, we aim to identify the primary determinants of proteotoxic stress response in PDSCs. We generated heat-induced dose-responsive proteotoxic stress models of three stem cell types: placental origin cells, the placenta-derived mesenchymal stem cells (pMSCs), maternal origin cells, the decidua parietalis mesenchymal stem cells (DPMSCs), and the maternal–fetal interface cells, decidua basalis mesenchymal stem cells (DBMSCs), and measured stress induction through biochemical and cell proliferation assays. RT-PCR array analysis of 84 genes involved in protein folding and protein quality control led to the identification of Hsp70 members HSPA1A and HSPA1B as the prominent ones among 17 significantly expressed genes and with further analysis at the protein level through Western blotting. A kinetic analysis of HSPA1A and HSPA1B gene and protein expression allowed a time series evaluation of stress response. As identified by protein expression, an active stress response is in play even at 24 h. More prominent differences in expression between the two homologs are detected at the translational level, alluding to a potential higher requirement for HSPA1B during proteotoxic stress response in PDSCs.

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“…ER stress activates three signaling arms of the UPR ER that are mediated by three ER-resident transmembrane proteins, inositol-requiring enzyme 1 (IRE1), PKR-like endoplasmic reticulum kinase (PERK), and activating transcription factor 6 (ATF6) (Hetz et al, 2020). The UPR ER signaling acts to manage ER stress and maintain cellular homeostasis, mainly through increasing chaperon-associated protein folding, attenuating protein translation, and enhancing ER-associated proteasomal de-gradation (ERAD) (Yan et al, 2020). Recent studies support crucial roles of the UPR ER in improving the survival of organisms under various stress conditions (Chadwick and Lajoie, 2019).…”

Section: The Unfolded Protein Response Of the Endoplasmic Reticulum (...mentioning

confidence: 99%

The landscape of aging

Cai

Song

et al. 2022

Sci. China Life Sci.

Self Cite

200

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Aging is characterized by a progressive deterioration of physiological integrity, leading to impaired functional ability and ultimately increased susceptibility to death. It is a major risk factor for chronic human diseases, including cardiovascular disease, diabetes, neurological degeneration, and cancer. Therefore, the growing emphasis on “healthy aging” raises a series of important questions in life and social sciences. In recent years, there has been unprecedented progress in aging research, particularly the discovery that the rate of aging is at least partly controlled by evolutionarily conserved genetic pathways and biological processes. In an attempt to bring full-fledged understanding to both the aging process and age-associated diseases, we review the descriptive, conceptual, and interventive aspects of the landscape of aging composed of a number of layers at the cellular, tissue, organ, organ system, and organismal levels.

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Protein quality control of cell stemness

Cited by 29 publications

References 183 publications

mRNA Translation Is Dynamically Regulated to Instruct Stem Cell Fate

mRNA Translation Is Dynamically Regulated to Instruct Stem Cell Fate

Heat-Induced Proteotoxic Stress Response in Placenta-Derived Stem Cells (PDSCs) Is Mediated through HSPA1A and HSPA1B with a Potential Higher Role for HSPA1B

The landscape of aging

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