Protein quality of Asian beans and their wild progenitor, Vigna sublobata (Roxb)

Khalil, Iftikhar Hussain; Khan, Saleem

doi:10.1016/0308-8146(95)92832-5

Cited by 22 publications

(15 citation statements)

References 11 publications

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“…Table 2 shows that the crude protein contents of V unguiculata seeds (195–261 g kg −1 dry matter) are comparable with those of several other cowpea varieties, which ranged from 210 to 270 g kg −1 dry matter 22–24. They are also close to the values reported for other species belong to the genus Vigna , such as V umbelata (172–181 g kg −1 dry matter),25 V campensis (224 g kg −1 dry matter),24 V mungo (238–250 g kg −1 dry matter),26, 27 V radiata (231 g kg −1 dry matter),27 V angularis (227 g kg −1 dry matter)28 and V minima (185–257 g kg −1 dry matter) 29. The high carbohydrate contents observed (678–761 g kg −1 dry matter) must have been overestimated, since fibre was not determined in the present study.…”

Section: Resultssupporting

confidence: 87%

Application of high‐resolution melting for genotyping bovine mitochondrial DNA

Zhou

Yan

et al. 2011

Biotechnology Progress

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Recent studies have demonstrated that mitochondrial DNA (mtDNA) haplotype has a significant impact on the efficiency of bovine somatic cell nuclear transfer. Conventional methods for detecting mtDNA variations and haplotypes, such as restriction fragment length polymorphism (RFLP), temporal temperature gradient gel electrophoresis, dHPLC and sequencing, are labor intensive or expensive and have low sensitivity. High-resolution melting (HRM) analysis is a new technique for mutation detection and has the advantages of speed, cost, and accuracy. Here, we describe the genotyping of bovine mtDNA using HRM analysis. DNA samples containing mtDNA were extracted from 75 Holstein cows and subjected to rapid-cycle (<20 min) PCR of small amplicons (<120 bp) using specific primer sets. Capillaries containing the PCR products were then subjected to HRM analysis; data were acquired in 2 min and analyzed using the instrument's software. Five common bovine mtDNA single nucleotide polymorphisms were identified: 9602 G>A, 169 A>G, 166A>G with 173A>G, and 363C>G. These results agree with both sequencing and RFLP analysis. In addition, a very small amount of heteroplasmic variants (<5%) was sufficiently to be distinguished by HRM analysis that would be very useful to differentiate heteroplasmy vs. homoplasmy. HRM analysis thus provides a new approach to genotyping bovine mtDNA sequence variations and has many advantages over other methods, including speed of analysis, cost, and accuracy. We believe this will be a valuable technique for determining the efficiency of nuclear transfer in cloned embryos and for studying maternal effects on nuclear-cytoplasm interactions.

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Section: Resultssupporting

confidence: 87%

Application of high‐resolution melting for genotyping bovine mitochondrial DNA

Zhou

Yan

et al. 2011

Biotechnology Progress

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“…Table 2 shows that the crude protein contents of V unguiculata seeds (195±261 g kg À1 dry matter) are comparable with those of several other cowpea varieties, which ranged from 210 to 270 g kg À1 dry matter. 22±24 They are also close to the values reported for other species belong to the genus Vigna, such as V umbelata (172±181 g kg À1 dry matter), 25 V campensis (224 g kg À1 dry matter), 24 V mungo (238±250 g kg À1 dry matter), 26,27 V radiata (231 g kg À1 dry matter), 27 V angularis (227 g kg À1 dry matter) 28 and V minima (185±257 g kg À1 dry matter). 29 The high carbohydrate contents observed (678±761 g kg À1 dry matter) must have been overestimated, since ®bre was not determined in the present study.…”

Section: Results and Discussion Seed Mean Weight And Dimensionsupporting

confidence: 74%

Proximate composition, amino acid content and haemagglutinating and trypsin-inhibiting activities of some BrazilianVigna unguiculata (L) Walp cultivars

Maia

Oliveira

Matos

et al. 2000

J. Sci. Food Agric.

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“…The crude protein content in our study seems to be higher than the previous ündings in Cajanus cajan (193·9 g kg~1) (Nwokolo 1987) and Vigna umbellata (210·0 g kg~1) (Rajaram and Janardhanan 1990). The crude fat content of B purpurea is found to be very high compared with Vigna radiata (22 g kg~1) and V mungo (18 g kg~1) (Khalil and Khan 1995). The seeds of B purpurea have a high energy value due to the presence of a high level of crude fat.…”

Section: Resultscontrasting

confidence: 51%

“…It is noteworthy that all the essential amino acids of seed proteins from B purpurea, excepting sulphur-containing amino acids, lysine and tryptophan register higher values than the FAO/WHO (1990) recommended pattern. Threonine and isoleucine are present in higher levels in B purpurea compared with various Vigna species (Khalil and Khan 1995). However, methionine content is comparable with that of Vigna radiata (Ignacimuthu and Babu 1987).…”

Section: Resultsmentioning

confidence: 93%