Protein separation techniques in the study of tissue regeneration

Pa, Tsonis; Rio‐Tsonis, Katia Del

doi:10.1016/0021-9673(94)00989-m

Cited by 5 publications

(1 citation statement)

References 7 publications

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“…Consistent with these expression patterns is that FGF can enhance transdifferentiation of pigment epithelial cells to lens cells in vitro (21). Consistent with a role of FGF in lens regeneration in vivo is that proteoglycans, proteins known to bind FGF, disappear sequentially from the dorsal iris after lentectomy (22,23). It could be that loss of proteoglycans renders FGF accessible for its function as mitogen after dedifferentiation.…”

Section: Resultsmentioning

confidence: 80%

Conservation of fibroblast growth factor function in lens regeneration

Rio‐Tsonis

Jung

Chiu

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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In urodele amphibians, lens induction during development and regeneration occurs through different pathways. During development, the lens is induced from the mutual interaction of the ectoderm and the optic vesicle, whereas after lentectomy the lens is regenerated through the transdifferentiation of the iris-pigmented epithelial cells. Given the known role of fibroblast growth factors (FGFs) during lens development, we examined whether or not the expression and the effects of exogenous FGF during urodele lens regeneration were conserved. In this paper, we describe expression of FGF-1 and its receptors, FGFR-2 (KGFR and bek variants) and FGFR-3, in newts during lens regeneration. Expression of these genes was readily observed in the dedifferentiating pigmented epithelial cells, and the levels of expression were high in the lens epithelium and the differentiating fibers and lower in the retina. These patterns of expression implied involvement of FGFs in lens regeneration. To further elucidate this function, we examined the effects of exogenous FGF-1 and FGF-4 during lens regeneration. FGF-1 or FGF-4 treatment in lentectomized eyes resulted in the induction of abnormalities reminiscent to the ones induced during lens development in transgenic mice. Effects included transformation of epithelial cells to fiber cells, double lens regeneration, and lenses with abnormal polarity. These results establish that FGF molecules are key factors in fiber differentiation, polarity, and morphogenesis of the lens during regeneration even though the regenerating lens is induced by a different mechanism than in lens development. In this sense, FGF function in lens regeneration and development should be regarded as conserved. Such conservation should help elucidate the mechanisms of lens regeneration in urodeles and its absence in higher vertebrates.

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Section: Resultsmentioning

confidence: 80%

Conservation of fibroblast growth factor function in lens regeneration

Rio‐Tsonis

Jung

Chiu

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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Regulation of lens regeneration by fibroblast growth factor receptor 1

et al. 1998

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A new integrated statistical approach to the diagnostic use of two‐dimensional maps

Marengo¹,

Robotti²,

Gianotti³

et al. 2003

Electrophoresis

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Two-dimensional (2-D) electrophoresis is a very useful technique for the analysis of proteins in biological tissues. The complexity of the 2-D maps obtained causes many difficulties in the comparison of different samples. A new method is proposed for comparing different 2-D maps, based on five steps: (i) the digitalisation of the image; (ii) the transformation of the digitalised map in a fuzzy entity, in order to consider the variability of the 2-D electrophoretic separation; (iii) the calculation of a similarity index for each pair of maps; (iv) the analysis by multidimensional scaling of the previously obtained similarity matrix; (v) the analysis by classification or cluster analysis techniques of the resulting map co-ordinates. The method adopted was first tested on some simulated samples in order to evaluate its sensitivity to small changes in the spots position and size. The optimal setting of the method parameters was also investigated. Finally, the method was successfully applied to a series of real samples corresponding to the electrophoretic bidimensional analysis of sera from normal and nicotine-treated rats. Multidimensional scaling allowed the separation of the two classes of samples without any misclassification.

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Protein separation techniques in the study of tissue regeneration

Cited by 5 publications

References 7 publications

Conservation of fibroblast growth factor function in lens regeneration

Conservation of fibroblast growth factor function in lens regeneration

Regulation of lens regeneration by fibroblast growth factor receptor 1

A new integrated statistical approach to the diagnostic use of two‐dimensional maps

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