Regulation of lens regeneration by fibroblast growth factor receptor 1

Rio‐Tsonis, Katia Del; Trombley, Michael T.; McMahon, Gerald; Tsonis, Panagiotis A.

doi:10.1002/(sici)1097-0177(199809)213:1<140::aid-aja14>3.0.co;2-6

Cited by 70 publications

(11 citation statements)

References 18 publications

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“…Fibroblast growth factors, known to play important roles in lens development, have also been shown to play important roles in the process of Wolffian lens regeneration in newts (i.e., FGF2, Kodama and Eguchi, 1995; Hyuga, et al, 1993; Del-Rio Tsonis et al, 1997, 1998; McDevitt et al, 1997) and in cornea-lens transdifferentiation in Xenopus (i.e., FGF1, Bosco et al, 1994, 1997). …”

Section: Discussionmentioning

confidence: 99%

Gene expression profiles of lens regeneration and development inXenopus laevis

Malloch

Perry

Fukui

et al. 2009

Developmental Dynamics

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Seven hundred and thirty-four unique genes were recovered from a cDNA library enriched for genes up-regulated during the process of lens regeneration in the frog Xenopus laevis. The sequences represent transcription factors, proteins involved in RNA synthesis/processing, components of prominent cell signaling pathways, genes involved in protein processing, transport, and degradation (e.g., the ubiquitin/proteasome pathway), matrix metalloproteases (MMPs), as well as many other proteins. The findings implicate specific signal transduction pathways in the process of lens regeneration, including the FGF, TGF-beta, MAPK, Retinoic acid, Wnt, and hedgehog signaling pathways, which are known to play important roles in eye/lens development and regeneration in various systems. In situ hybridization revealed that the majority of genes recovered are expressed during embryogenesis, including in eye tissues. Several novel genes specifically expressed in lenses were identified. The suite of genes was compared to those up-regulated in other regenerating tissues/organisms, and a small degree of overlap was detected.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiles of lens regeneration and development inXenopus laevis

Malloch

Perry

Fukui

et al. 2009

Developmental Dynamics

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“…In the future, we will utilize several candidate genes involved in determining dorsal and ventral identities. For example, McDevitt et al (1997) and Del Rio‐Tsonis et al (1998) have demonstrated that the fibroblast growth factor receptor is localized in the newt dorsal iris. It has also been reported that the gene containing T‐box is expressed only on the dorsal side of the eye (Koshiba‐Takeuchi et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Highly efficient transfection system for functional gene analysis in adult amphibian lens regeneration

et al. 2001

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The analysis of newt lens regeneration has been an important subject in developmental biology. Recently, it has been reported that the genes involved in the normal eye development are also expressed in the regenerative process of lens regeneration in the adult newt. However, functional analysis of these genes has not been possible, because there is no system to introduce genes efficiently into the cells involved in the regeneration. In the present study, lipofection was used as the method for gene transfer in cultured pigmented iris cells that can transdifferentiate into lens cells in newt lens regeneration. Positive expression of a reporter gene was obtained in more than 70% of cells. In addition, the aggregate derived from gene-transfected cells maintained its expression at a high level for a long time within the host tissue. To verify the effectiveness of this model system with a reporter gene in lens regeneration, Pax6, which is suggested to be involved in normal eye development and lens regeneration, was transfected. Ectopic expression of lens-specific crystallins was obtained in cells that show no such activity in normal lens regeneration. These results made it possible for the first time to analyze the molecular mechanism of lens regeneration in the adult newt.

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“…All these factors are expressed in the differentiating lens vesicle and the regenerating lens. However, the strongest evidence for spatial regulation was received from the studies involving pax‐6, prox‐1, and FGFR‐1 (Del Rio‐Tsonis et al, 1995, 1997, 1998, 1999; McDevitt et al, 1997; Mizuno et al, 1999b). All of these factors were found to be specific in the dorsal iris.…”

Section: Lens Regenerationmentioning

confidence: 99%

“…The products of prox‐1 and FGFR‐1 were found to be regulated in the dorsal iris as well (Del Rio‐Tsonis et al, 1998, 1999, Mizuno et al, 1999b). In addition, an inhibitor to FGFR‐1 was able to abolish regeneration from the dorsal iris (Del Rio‐Tsonis et al, 1998). Another interesting result was obtained by inhibiting the activity of RARs with a specific inhibitor.…”

Section: Lens Regenerationmentioning

confidence: 99%

Eye regeneration at the molecular age

Rio‐Tsonis

Tsonis

2003

Developmental Dynamics

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160

114

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Eye tissues such as the lens and the retina possess remarkable regenerative abilities. In amphibians, a complete lens can be regenerated after lentectomy. The process is a classic example of transdifferentiation of one cell type to another. Likewise, retina can be regenerated, but the strategy used to replace the damaged retina differs, depending on the animal system and the age of the animal. Retina can be regenerated by transdifferentiation or by the use of stem cells. In this review, we present a synthesis on the regenerative capacity of eye tissues in different animals with emphasis on the strategy and the molecules involved. In addition, we stress the place of this field at the molecular age and the importance of the recent technologic advances. Developmental Dynamics 226:211-224, 2003.

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Regulation of lens regeneration by fibroblast growth factor receptor 1

Cited by 70 publications

References 18 publications

Gene expression profiles of lens regeneration and development inXenopus laevis

Gene expression profiles of lens regeneration and development inXenopus laevis

Highly efficient transfection system for functional gene analysis in adult amphibian lens regeneration

Eye regeneration at the molecular age

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