Proteins, lethality and in vivo effects of Iurus dufoureius asiaticus scorpion venom

Özkan, Özcan; Çiftçi, Gülay; Pekmezci, Gökmen Zafer; Kar, Sırrı; Uysal, Hamdi; Karaer, Kadri

doi:10.1016/j.toxicon.2007.04.010

Cited by 19 publications

(11 citation statements)

References 16 publications

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“…These symptoms were dose dependent; the high intensity of symptoms is mostly observed during the injection of elevated doses ranged from 0.7 to 1 mg/kg. Anterior data report the same finding after Intracerebroventricular injection of Iurus dufoureius asiaticus venom (Ozkan et al, 2007). The mice showed excitability, rapidly walking as humpback, excessive salivation, weakness, paralysis, coma and resulting in death.…”

Section: Discussionmentioning

confidence: 52%

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Behavioral, histopathological and biochemical impairments observed in mice envenomed by the scorpion: Hottentota gentili (Pallary, 1924)

Hidan

Touloun

Hiba

et al. 2015

Toxicon

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Section: Discussionmentioning

confidence: 52%

“…1) scorpions by electrical stimulation of the telson as described by (Ozkan et al 2007). The venom was diluted by sterile double distilled water and the protein content of venom was determined according to the method of Bradford (1976).…”

Section: Venom Extractionmentioning

confidence: 99%

Behavioral, histopathological and biochemical impairments observed in mice envenomed by the scorpion: Hottentota gentili (Pallary, 1924)

Hidan

Touloun

Hiba

et al. 2015

Toxicon

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“…Our work also attempted to deepen the in vivo activity of BotAF. Toxicity of the molecule was investigated by means of lethality assessment after 48 h and by direct observation of any mice behavioral change that could reflect a form of intoxication summarized in bibliographic data in which these symptoms were well characterized (Ozkan et al, 2007;Pipelzadeh et al, 2015). Besides toxicity, it was also important to assess animal motor response in BotAF administration condition.…”

Section: Discussionmentioning

confidence: 99%

BotAF, a new Buthus occitanus tunetanus scorpion toxin, produces potent analgesia in rodents

Maatoug

Jebali

Guieu

et al. 2018

Toxicon

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This work reports the purification of new potent scorpion neuropeptide, named BotAF, by an activity-guided screening approach. BotAF is a 64-residue long-chain peptide that shares very high similarity with the original β-like scorpion toxin group, in which several peptides have been characterized to be anti-nociceptive in rodents. BotAF administration to rodents does not produce any toxicity or motor impairment, including at high doses. In all models investigated, BotAF turned out to be an efficient peptide in abolishing acute and inflammatory (both somatic and visceral) pain in rodents. It performs with high potency compared to standard analgesics tested in the same conditions. The anti-nociceptive activity of BotAF depends on the route of injection: it is inactive when tested by i.c.v. or i.v. routes but gains in potency when pre-injected locally (in the same compartment than the irritant itself) or by i.t. root 40 to 60 min before pain induction, respectively. BotAF is not an AINS-like compound as it fails to reduce inflammatory edema. Also, it does not activate the opioidergic system as its activity is not affected by naloxone. BotAF does also not bind onto RyR and has low activity towards DRG ion channels (particularly TTX sensitive Na channels) and does not bind onto rat brain synaptosome receptors. In somatic and visceral pain models, BotAF dose-dependently inhibited lumbar spinal cord c-fos/c-jun mRNA up regulation. Altogether, our data favor a spinal or peripheral anti-nociceptive mode of action of BotAF.

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“…For these reasons, two previous studies reporting electrophoretic profiles of I. dufoureius asiaticus soluble venom have been revised again based on the new systematic literatures. Additionally, Ozkan et al (2007b) collected P. kraepelini from Mugla Province and misidentified it also as I. d. asiaticus. Additionally, Ozkan et al (2007b) collected P. kraepelini from Mugla Province and misidentified it also as I. d. asiaticus.…”

Section: Iurus Kinzelbachi and Protoiurus Kraepelinimentioning

confidence: 99%

Scorpion Venoms

2015

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In recent years, the field of toxinology has expanded substantially. On the one hand it studies venomous animals, plants and micro organisms in detail to understand their mode of action on targets. While on the other, it explores the biochemical composition, genomics and proteomics of toxins and venoms to understand their three interaction with life forms (especially humans), development of antidotes and exploring their pharmacological potential. Therefore, toxinology has deep linkages with biochemistry, molecular biology, anatomy and pharmacology. In addition, there is a fast-developing applied subfield, clinical toxinology, which deals with understanding and managing medical effects of toxins on human body. Given the huge impact of toxin-based deaths globally, and the potential of venom in generation of drugs for so-far incurable diseases (for example, diabetes, chronic pain), the continued research and growth of the field is imminent. This has led to the growth of research in the area and the consequent scholarly output by way of publications in journals and books. Despite this ever-growing body of literature within biomedical sciences, there is still no all-inclusive reference work available that collects all of the important biochemical, biomedical and clinical insights relating to toxinology. Composed of 11 volumes, Toxinology provides comprehensive and authoritative coverage of the main areas in toxinology, from fundamental concepts to new developments and applications in the field. Each volume comprises a focused and carefully chosen collection of contributions from leading names in the subject.

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Proteins, lethality and in vivo effects of Iurus dufoureius asiaticus scorpion venom

Cited by 19 publications

References 16 publications

Behavioral, histopathological and biochemical impairments observed in mice envenomed by the scorpion: Hottentota gentili (Pallary, 1924)

Behavioral, histopathological and biochemical impairments observed in mice envenomed by the scorpion: Hottentota gentili (Pallary, 1924)

BotAF, a new Buthus occitanus tunetanus scorpion toxin, produces potent analgesia in rodents

Scorpion Venoms

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