Proteo-Molecular Investigation of Cultivated Rice, Wild Rice, and Barley Provides Clues of Defense Responses against Rhizoctonia solani Infection

Shamim, Md.; Sharma, Divakar; Bisht, Deepa; Maurya, Rashmi; Kaashyap, Mayank; Srivastava, Deepti; Mishra, Anurag; Kumar, Deepak; Kumar, Mahesh; Juturu, Vijaya; Khan, Nafees A.; Chaudhary, Sameer; Hussain, Raja Rizwan; Singh, Kritika

doi:10.3390/bioengineering9100589

Cited by 4 publications

(4 citation statements)

References 57 publications

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“…The results and several physiological indices demonstrated that the antioxidative enzyme-related gene or protein is up-regulated at the transcriptional and translational levels. This finding corresponds with the results of omics studies on resistant cultivated rice, wild rice, and barley after Rhizoctonia solani infection ( Shamim et al., 2022 ). Furthermore, M. oryzae infection increases ROS production in MWG, stimulating antioxidant enzymes to counteract oxidative damage and detoxify ROS.…”

Section: Discussionsupporting

confidence: 89%

Integrative transcriptomic, proteomic, and phosphoproteomic analysis on the defense response to Magnaporthe oryzae reveals different expression patterns at the molecular level of durably resistant rice cultivar Mowanggu

et al. 2023

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Rice blast, caused by Magnaporthe oryzae is one of the most destructive diseases of rice (Oryza sativa L.) in most rice-cultivated areas worldwide. Mowanggu (MWG) is a traditional landrace rice variety in Yunnan with broad-spectrum and durable blast resistance against rice blast fungus. However, the underlying disease-resistance mechanisms remain unknown. An integrative transcriptomic, proteomic, and phosphoproteomic analysis of MWG was performed after inoculation with M. oryzae in this study. The transcriptomic and proteomic results revealed that MWG was moderately correlated at the transcriptional and protein levels. Differentially expressed genes and proteins were up-regulated and significantly enriched in protein phosphorylation, peroxisome, plant-pathogen interactions, phenylpropanoid metabolism and phenylalanine biosynthesis pathways. The phosphoproteomic profile and phosphorylated-protein-interaction network revealed that the altered phosphoproteins were primarily associated with reactive oxygen species (ROS), glycolysis, MAPK signaling pathways, and amino acid biosynthesis. In addition, a series of physiological and biochemical parameters, including ROS, soluble sugars, soluble protein and callus accumulation and defense-related enzyme activities, were used to validate the possible blast resistance mechanisms of MWG. The integrative transcriptomic, proteomic, and phosphoproteomic analysis revealed the different expression patterns at the molecular level of the durably resistant rice cultivar MWG after inoculation with M. oryzae, which provides insight into the molecular mechanisms of rice blast resistance.

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Section: Discussionsupporting

confidence: 89%

Integrative transcriptomic, proteomic, and phosphoproteomic analysis on the defense response to Magnaporthe oryzae reveals different expression patterns at the molecular level of durably resistant rice cultivar Mowanggu

et al. 2023

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“…Fourteen defense genes i.e. chitinase, gibberellin signal transduction and NBS-LRR encoding genes were present in the QTL regions qShB5.1, qShB5.2 that were reported to be responsible for ShB resistance in transgenic rice plant (Baisakh et (Shamim et al 2022). In conclusion, the study has identi ed the important QTLs and underlying candidate genes that might play important role in further understanding genetics of sheath blight resistance in rice and development of sheath blight resistant cultivars.…”

Section: Discussionmentioning

confidence: 89%

QTL mapping and candidate gene mining for sheath blight resistance in rice (Oryza sativa L.) using QTLseqr approach

Thesiya,

Lore,

Bhatia

et al. 2024

Preprint

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Sheath blight (ShB) caused by Rhizoctonia solani is a devastating disease that poses a major threat to rice (Oryza sativa L.) production worldwide. In this study, next generation sequencing assisted bulk segregant analysis (BSA) integrated with R package i.e. QTLseqr was utilized to identify QTL regions controlling the sheath blight resistance trait. F3 mapping progenies for ShB resistance trait was derived from the cross between susceptible rice cultivar PR121 and resistant donor IET 22769. Based on sheath blight screening of F3 progenies under artificial inoculation conditions, fifteen resistant (20-30 cm lesion height) and fifteen highly susceptible (70-85 cm lesion height) progenies were selected. DNA of the selected progenies were extracted and bulked respectively to constitute ShB-R and ShB-S bulks respectively. The two bulks along with parents were sequenced at > 20 X read depth. A total of 11,45,820 high-quality single nucleotide polymorphism (SNPs) were used for QTL-seq analysis using QTLseqr package. QTL analysis identified five QTLs namely qShB1, qShB3, qShB5.1, qShB5.2 and qShB6 on chromosome 1, 3, 5 and 6, respectively for resistance to ShB. A total of 69 candidate genes were identified within the QTL regions including leucine-rich repeat receptor-like kinase, coiled-coil nucleotide-binding and transcription factor protein etc. which might play a significant role in defense mechanism against R. solani. The identified QTLs and candidate genes can be further studied to understand genetics of ShB resistance in rice and to develop ShB resistant varieties.

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“…was inoculated against sheath blight disease that increase in the chlorophyll content of leaves was observed (Tamreihao et al 2016). The studies in sheath blight disease based on iTRAQ based proteomics to identify the major mechanisms of resistant varieties against sheath blight showed that increased expression of chlorophyl a-b binding protein 2 during the R. solani infection in resistant varieties (Feng et al 2022, Shamim et al 2022). The resistant varieties thus tends to increase chlorophyll content under sheath blight pathogen infection, which correlated well with our results that S. hyderabadensis inoculation increased the expression of chlorophyll a-b binding protein 2.…”

Section: Discussionmentioning

confidence: 99%

Shift in SAR to ISR during the Rice- R. Solani interaction mediated by Streptomyces hyderabadensis confers sheath blight resistance in susceptible genotype

Arun

Krishnaraj

Prashanthi

et al. 2023

Preprint

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Rice sheath blight disease is caused by the necrotrophic pathogen Rhizoctonia solani Kuhn (teleomorph; Thanatephorus cucumeris). Several studies have reported the disease suppression mechanisms in resistant varieties based on innate and systemic acquired resistance (SAR) mechanisms. The resistant varieties turn susceptible due to the intelligence of pathogen strains to mimic plant defense signaling mechanisms. An alternative and ecofriendly approach to tackle the negative effects of plant-pathogen interaction is the application of bio-control agents. Actinobacteria is known for production of secondary metabolites under stress that initiates pre-signaling to enable induced immunity at early plant stages to tackle the pathogen attack during the later stages of plant development. The current study is focused on understanding of mechanisms that provide resistance to rice plants against R. solani in presence of actinobacteria, Streptomyces hyderabadensis, based on biochemical and mRNA/transcript level analysis. The sheath blight incidence was significantly reduced and the disease score was maintained at 1 (lesion height less than 10%) compared to pathogen control. Biochemical analysis revealed that the actinobacterial inoculation enhanced the levels of phenyl ammonia lyase, phenol, polyphenol oxidases, catalases and peroxidases during the tripartite interaction that provided initial resistance and protection from ROS generated during pathogen infection through detoxification process. During the interaction, higher expression of chitinase gene, improvement in chlorophyll content by the expression of chlorophyll a-b binding protein, maintenance of plant overall development by maintaining the balance of melatonin production, lower expression of SAR supportive genes (PAL, ICS, GST) at the later intervals, expression of signaling proteins (14-3-3 like protein GF14-E) to activate the defense related proteins and the proteins that supports the SAR to ISR shift (NPR1) in presence of S. hyderabadensis under pathogen pressure of R. solani.

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Proteo-Molecular Investigation of Cultivated Rice, Wild Rice, and Barley Provides Clues of Defense Responses against Rhizoctonia solani Infection

Cited by 4 publications

References 57 publications

Integrative transcriptomic, proteomic, and phosphoproteomic analysis on the defense response to Magnaporthe oryzae reveals different expression patterns at the molecular level of durably resistant rice cultivar Mowanggu

Integrative transcriptomic, proteomic, and phosphoproteomic analysis on the defense response to Magnaporthe oryzae reveals different expression patterns at the molecular level of durably resistant rice cultivar Mowanggu

QTL mapping and candidate gene mining for sheath blight resistance in rice (Oryza sativa L.) using QTLseqr approach

Shift in SAR to ISR during the Rice- R. Solani interaction mediated by Streptomyces hyderabadensis confers sheath blight resistance in susceptible genotype

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