Proteolysis of bovine α-lactalbumin by thermolysin during thermal denaturation

Influence of buffer type and concentration on the peptide composition of trypsin hydrolysates of β-lactoglobulin

“…3, it can also be seen (as expected) that the peptide peaks increased in size between 5 and 15 min but decreased by 30 min. This phenomenon has been reported by other workers (N'Negue et al, 2006;Schmelzer et al, 2007) who found that thermolysin and pepsin hydrolysed proteins to release intermediate peptides which were attacked later on. The reduction in size of the peptide peaks is, therefore accounted for by further cleavages, marked by production of smaller peptides associated with nonspecific activity (results not shown).…”

Protein enzymatic hydrolysis integrated with ultrafiltration: Thermolysin application in obtaining peptides

“…The gathered data support the claim about pre-incubation influence on the proteolysis process efficiency, but for BSA the effect at 70°C was different than observed for a-lactalbumin [26]. We have observed that pre-incubation at 46°C and 60°C contributed to an increase of the hydrolysis rate and final efficiency (checked after 24 and 48 h); at 60°C the increase was more than twice as large - Table 3.…”

“…Negue and co-workers [26] showed the diverse effect of a-lactalbumin hydrolysis carried out at 25°C and in denaturation conditions (70°C). We studied the pre-incubation of albumin at 46, 60 and 70°C for 0.25, 0.5 and 1 h, respectively.…”

“…Thermolysin is known to act at high temperatures [26]. However, at 70°C BSA underwent coagulation and in this form it was not susceptible to hydrolysis.…”

Fractionation and identification of ACE-inhibitory peptides from α-lactalbumin and β-casein produced by thermolysin-catalysed hydrolysis

“…In most cases, one or two cycles of successive RP-HPLC separation would be satisfactory to isolate the peptides in sufficient purity to identify them by mass spectrometry and/or amino acid compositional analysis (Abubakar, Saito, Kitazawa, Kawai, & Itoh, 1998;N'Negue et al, 2006).…”