Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals

Kaneko, Hirofumi; Kamiie, Junichi; Kawakami, Hirotaka; Anada, Takahisa; Honda, Yoshitomo; Shiraishi, Naoki; Kamakura, Shinji; Terasaki, Tetsuya; Shimauchi, Hidetoshi; Сузукі, Осаму

doi:10.1016/j.ab.2011.07.022

Cited by 47 publications

(39 citation statements)

References 67 publications

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“…and (B)]. This result indicates that the presence of OCP promotes osteoclast formation not only due to the increase of Pi in the media, but also other physicochemical properties, such as crystal structures and a progressive transformation process, including dissolution and surface modification concomitant with serum protein adsorption …”

Section: Discussionmentioning

confidence: 79%

“…This result indicates that the presence of OCP promotes osteoclast formation not only due to the increase of Pi in the media, but also other physicochemical properties, such as crystal structures and a progressive transformation process, including dissolution and surface modification concomitant with serum protein adsorption. [7][8][9]64 The dissolution and possible precipitation behavior of CaPs during osteoclast culture were significantly influenced by the HA content of CaPs. Pi ions were higher in the order of HA60OCP40, HA20OCP80, and OCP, and tended to decrease as the days of osteoclast culture increased [ Fig.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3] Observations that partial hydrolysis of OCP enhances bone formation more than that of the original salt supports this proposition. 4 Analyses of OCP under physiological conditions have shown that OCP induces dynamic physicochemical processes, including inorganic ion exchange, 5,6 serum proteins adsorption, [7][8][9] and a progressive crystallographic change from OCP to HA without the involvement of phases other than OCP and HA. 7,10 Recent chemical studies have shown that calcium phosphate phases can be controlled primarily through the degree of solution supersaturation, but are also influenced by the nucleation rate and the growth rate of the crystal phases.…”

Section: Introductionmentioning

confidence: 99%

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Effect of calcium phosphate phases affecting the crosstalk between osteoblasts and osteoclasts in vitro

Shiwaku

Tsuchiya

Xiao

et al. 2019

J Biomedical Materials Res

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Previous studies have reported that octacalcium phosphate (OCP) enhances osteoblast differentiation and osteoclast formation during the hydrolysis process to hydroxyapatite (HA). However, the crystal phases that affect the crosstalk between osteoclasts and osteoblasts are unknown, which should determine the bone substitute material's property of OCP. The present study was designed to investigate whether the chemical composition and crystal structure of calcium phosphates affect osteoclast formation and the osteoclast–osteoblast crosstalk. Biodegradable β‐tricalcium phosphate (β‐TCP) was used as the control material. Osteoclasts were cultured on HA/OCP or HA/TCP disks and their cellular responses were assessed. Both OCP and β‐TCP had a similar ability to create multinucleated osteoclasts. However, OCP promoted the expression of complement component 3a (C3a), a positive coupling factor, in osteoclasts, whereas β‐TCP enhanced that of EphrinB2 (EfnB2) and collagen triple helix repeat containing 1 (Cthrc1). During osteoclast culture, phosphate ions were released from the crystals, and OCP‐HA conversion was advanced in HA/OCP mixtures and OCP. X‐ray diffraction analysis revealed no remarkable changes in the crystal structures of HA/TCP mixtures and β‐TCP before and after osteoclast culture. These results indicate that the distinct chemical environment induced by the calcium phosphate phases affects the crosstalk between osteoclasts and osteoblasts. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1001–1013, 2019.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of calcium phosphate phases affecting the crosstalk between osteoblasts and osteoclasts in vitro

Shiwaku

Tsuchiya

Xiao

et al. 2019

J Biomedical Materials Res

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“…We previously reported that OCP combined with bone morphogenetic protein‐2 (BMP‐2) synergistically enhanced bone regeneration more than OCP alone, while the dose of BMP‐2 used did not affect bone regeneration by OCP 28. Furthermore, a proteome analysis by liquid chromatography tandem mass spectrometry indicated that OCP granules incubated in rat serum proteins selectively adsorbed bone formation‐related proteins, such as apolipoprotein E 29. These findings suggest that some bone formation‐related proteins adsorb OCP/Col, and adsorbed proteins may then promote bone formation.…”

Section: Discussionmentioning

confidence: 99%

Single‐dose local administration of teriparatide with a octacalcium phosphate collagen composite enhances bone regeneration in a rodent critical‐sized calvarial defect

et al. 2017

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Octacalcium phosphate and collagen composite (OCP/Col) achieves stable bone regeneration without cell transplantation in preclinical studies. Recently, a sponsor‐initiated clinical trial was conducted to commercialize the material. The present study investigated bone regeneration by OCP/Col with the single local administration of teriparatide (parathyroid hormone 1‐34; TPTD). OCP/Col was prepared by mixing sieved granules of OCP and atelocollagen for medical use and a disk was molded. After the creation of a rodent critical‐sized calvarial defect, OCP/Col or OCP/Col with dripped TPTD solution (1.0 or 0.1 µg; OCP/Col/TPTDd1.0 or OCP/Col/TPTDd0.1) was implanted into the defect. Six defects in each group were fixed 12 weeks after implantation. Radiographic examinations indicated that radiopaque figures in defects treated with OCP/Col with TPTD (OCP/Col/TPTDd) occupied a wider range than those treated with OCP/Col. Histological results demonstrated that most of the defect in OCP/Col/TPTDd was filled with newly formed bone. A histomorphometrical examination indicated that the percentage of newly formed bone was significantly higher in the defects of OCP/Col/TPTDd 1.0 (53.6 ± 4.3%) and OCP/Col/TPTDd 0.1 (52.2 ± 7.4%) than in those of OCP/Col (40.1 ± 8.4%), whereas no significant differences were observed between OCP/Col/TPTDd1.0 and OCP/Col/TPTDd0.1. These results suggest that OCP/Col with the single local administration of TPTD enhances bone regeneration in a rodent calvarial critical‐sized bone defect. © 2017 The Authors Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1851–1857, 2018.

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“…9,12 OCP has been classified as an osteoconductive material as well as HA, b-TCP and glass ceramic materials. 19 The mechanism to enhance these cellular activities has been proposed to be stemmed from the intrinsic chemical property of OCP which can be progressively converted to Ca-deficient HA under physiological condition 14 and provide a suitable surface for cell attachment and differentiation through inorganic ionic dissolution 20,21 and protein interactions 22,23 between the tissue fluid and the hydrolyzing surfaces. 19 The mechanism to enhance these cellular activities has been proposed to be stemmed from the intrinsic chemical property of OCP which can be progressively converted to Ca-deficient HA under physiological condition 14 and provide a suitable surface for cell attachment and differentiation through inorganic ionic dissolution 20,21 and protein interactions 22,23 between the tissue fluid and the hydrolyzing surfaces.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic cellular activity around onlaid octacalcium phosphate‐gelatin composite onto rat calvaria

Iwama

Anada

Tsuchiya

et al. 2018

J Biomedical Materials Res

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The activity and the distribution of osteogenic cells around octacalcium phosphate (OCP) granules combined with gelatin matrix (OCP/Gel) onlaid on rat calvaria were analyzed histomorphometrically and immunohistochemically during vertical bone augmentation under mechanical or nonmechanical environment until 8 weeks. OCP/Gel disk was placed in subperiosteal pocket on the calvaria with or without polytetrafluoroethylene (PTFE) support. The latter is a nonmechanical stress model to alleviate the mechanical stress from the subcutaneous tissues. Onlay grafts of gelatin (Gel) sponge disk and OCP granules were also carried out for the comparison purpose. When bone augmentation was evaluated in first area from bone surface (area until 150 μm high from bone surface) and second area above the newly formed bone (area until 150 µm high from the first area), bone formation was enhanced most in first area followed by second area of OCP/Gel with PTFE. The appearance of tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells was suppressed more in the newly formed tissue with PTFE than those without PTFE with an emphasis of the presence of gelatin. Although Runx2 positive-cells were accumulated more in both OCP/Gel with and without PTFE, osteocalcin-positive cells were abundant in OCP/Gel with PTFE than that without PTFE, suggesting that nonmechanical stress condition is more suitable for osteoblast differentiation. The appearance of receptor activator of nuclear factor (NF)-kappaB ligand (RANKL)-positive cells was restrained in OCP/Gel and Gel with PTFE while osteoprotegerin-positive cells were most accumulated in OCP/Gel without PTFE. The results suggest that OCP/Gel composite under the mechanical stress-alleviated condition can enhance bone augmentation through balanced osteoblastic and osteoclastic cellular activities. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1322-1333, 2018.

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Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals

Cited by 47 publications

References 67 publications

Effect of calcium phosphate phases affecting the crosstalk between osteoblasts and osteoclasts in vitro

Effect of calcium phosphate phases affecting the crosstalk between osteoblasts and osteoclasts in vitro

Single‐dose local administration of teriparatide with a octacalcium phosphate collagen composite enhances bone regeneration in a rodent critical‐sized calvarial defect

Osteogenic cellular activity around onlaid octacalcium phosphate‐gelatin composite onto rat calvaria

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