Proteome and phospholipid alteration reveal metabolic network of Bacillus thuringiensis under triclosan stress

Li, Yang; Li, Chongshu; Qin, Huaming; Yang, Meng; Ye, Jinshao; Long, Yan; Ou, Huase

doi:10.1016/j.scitotenv.2017.10.004

Cited by 21 publications

(4 citation statements)

References 35 publications

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“…High levels of TCS were recently found in human urine, blood sample, liver, adipose tissue, brain and breast milk (Ruszkiewicz et al, 2017) and its structure is similar to the polychlorinated phenoxyphenols that, under oxidizing conditions, cyclize to other toxic byproducts, which present very harmful effects on human health (Solá-Gutiérrez et al, 2018). Furthermore, a recent proteomic study (Li et al, 2018) revealed a distinct evidence of the potential impact of TCS on human metabolic pathways.…”

Section: Introductionmentioning

confidence: 99%

Environmental concentrations of triclosan activate cellular defence mechanism and generate cytotoxicity on zebrafish (Danio rerio) embryos

Parenti

Ghilardi

Torre

et al. 2019

Science of The Total Environment

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Section: Introductionmentioning

confidence: 99%

Environmental concentrations of triclosan activate cellular defence mechanism and generate cytotoxicity on zebrafish (Danio rerio) embryos

Parenti

Ghilardi

Torre

et al. 2019

Science of The Total Environment

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“…Therefore, to a large extent, the up-regulation of phospholipid metabolism could promote the formation of the membrane and reflect the production of biomass eventually. Since only a few biomarker metabolites have been detected, we speculate that variation in the phospholipid metabolic pathway might not have the significant effect on biomass production [28,33].…”

Section: Phospholipid Metabolism: Lipid Biosynthesis Form Cellular Membranesmentioning

confidence: 96%

“…For most microorganisms, the intensity of intracellular nucleotide metabolism reflects the growth of organelles and the production of biomass [28,29]. The down-regulated adenine, adenosine monophosphate (AMP), and guanosine monophosphate indicated that nucleotide biosynthesis was sharply decreased in IP compared with TP (Figure 4), which is consistent with the previous study that has demonstrated that the effect of the stress-induced by different plenty of Insol-P on biomass production can be characterized by antagonistic or synergistic interactions [27].…”

Section: Purine and Nucleotide Metabolism: Biomass Productionmentioning

confidence: 99%

Intracellular Metabolomics Switching Alters Extracellular Acid Production and Insoluble Phosphate Solubilization Behavior in Penicillium oxalicum

Jiang

et al. 2020

Metabolites

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This research aims to understand the precise intracellular metabolic processes of how microbes solubilize insoluble phosphorus (Insol-P) to increase bio-available P. Newly isolated Penicillium oxalicum PSF-4 exhibited outstanding tricalcium phosphate (TP) and iron phosphate (IP) solubilization performance—as manifested by microbial growth and the secretion of low-molecular-weight organic acids (LMWOAs). Untargeted metabolomics approach was employed to assess the metabolic alterations of 73 intracellular metabolites induced by TP and IP compared with soluble KH2PO4 in P. oxalicum. Based on the changes of intracellular metabolites, it was concluded that (i) the enhanced intracellular glyoxylate and carbohydrate metabolisms increased the extracellular LMWOAs production; (ii) the exposure of Insol-P poses potential effects to P. oxalicum in destructing essential cellular functions, affecting microbial growth, and disrupting amino acid, lipid, and nucleotide metabolisms; and (iii) the intracellular amino acid utilization played a significant role to stimulate microbial growth and the extracellular LMWOAs biosynthesis.

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“…Endoenzymes, an appropriate amount of exogenous nutrients, and surfactants can accelerate TPT degradation [8][9][10]. Furthermore, the protein expression of B. thuringiensis and its metabolic network related to the degradation of pollutants, such as erythromycin and triclosan, have been revealed by using omics approaches [11,12]. However, the mechanisms by which B. Thuringiensis recognizes and adsorbs copper after TPT treatment have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Molecular Recognition and Cell Surface Biochemical Response of Bacillus thuringiensis on Triphenyltin

Zhang

Qin

et al. 2019

Processes

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Triphenyltin (TPT) has severely polluted the environment, and it often coexists with metal ions, such as Cu2+. This paper describes the cell’s molecular recognition of TPT, the interaction between TPT recognition and Cu2+ biosorption, and their effect on cell permeability. We studied the recognition of TPT by Bacillus thuringiensis cells and the effect of TPT recognition on Cu2+ biosorption by using atomic force microscopy to observe changes in cell surface mechanical properties and cellular morphology and by using flow cytometry to determine the cell growth status and cell permeability. The results show that B. thuringiensis can quickly recognize different media. The adhesion force of cells in contact with Tween 80 was significantly reduced to levels that were much lower than that of cells in contact with PBS. Conversely, the cell surface adhesion force increased as TPT became more degraded. B. thuringiensis cells maintained their original morphology after 48 h of TPT treatment. The amount of Cu2+ adsorption by TPT-treated cells was positively correlated with the surface adhesion force (r = 0.966, P = 0.01). The cell adhesion force significantly decreased after Cu2+ adsorption, and cell recognition of TPT and/or Cu2+ hindered the entrance of 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) into the cell. The initial diffusion time of DCFH-DA into cells treated by PBS, Cu2+, TPT, and TPT+Cu2+ was 4, 10, 30, and 30 min, respectively, and the order of the fluorescence intensity was PBS >> Cu2+ > TPT > TPT+Cu2+. We conclude that changes in the cell surface properties of the microbe during recognition of pollutants depend on the contaminant’s properties. B. thuringiensis recognized TPT and secreted intracellular substances that not only enhanced the adsorption of Cu2+, but also formed a “barrier” on the cell surface that reduced permeability. These findings provide a novel insight into the mechanism of microbial removal of pollutants.

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Proteome and phospholipid alteration reveal metabolic network of Bacillus thuringiensis under triclosan stress

Cited by 21 publications

References 35 publications

Environmental concentrations of triclosan activate cellular defence mechanism and generate cytotoxicity on zebrafish (Danio rerio) embryos

Environmental concentrations of triclosan activate cellular defence mechanism and generate cytotoxicity on zebrafish (Danio rerio) embryos

Intracellular Metabolomics Switching Alters Extracellular Acid Production and Insoluble Phosphate Solubilization Behavior in Penicillium oxalicum

Molecular Recognition and Cell Surface Biochemical Response of Bacillus thuringiensis on Triphenyltin

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