2012
DOI: 10.1104/pp.112.203679
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Proteomic Analysis of Chloroplast-to-Chromoplast Transition in Tomato Reveals Metabolic Shifts Coupled with Disrupted Thylakoid Biogenesis Machinery and Elevated Energy-Production Components  

Abstract: A comparative proteomic approach was performed to identify differentially expressed proteins in plastids at three stages of tomato (Solanum lycopersicum) fruit ripening (mature-green, breaker, red). Stringent curation and processing of the data from three independent replicates identified 1,932 proteins among which 1,529 were quantified by spectral counting. The quantification procedures have been subsequently validated by immunoblot analysis of six proteins representative of distinct metabolic or regulatory p… Show more

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Cited by 118 publications
(148 citation statements)
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(138 reference statements)
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“…Additionally, our results highlight several aspects of the regulation of global reorganization during chromoplast differentiation. The change pattern in protein abundance reported here is partially in agreement with the chromoplast differentiation in tomato (Barsan et al, 2012) but shows great differences from the proplastid/ etioplast-to-chloroplast transition in maize/rice (Kleffmann et al, 2007;Majeran et al, 2010). The overall findings from this study provide useful information about chromoplast biogenesis in sweet orange flesh and facilitate a better understanding of its chromoplast differentiation and senescence.…”
Section: Resultssupporting
confidence: 78%
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“…Additionally, our results highlight several aspects of the regulation of global reorganization during chromoplast differentiation. The change pattern in protein abundance reported here is partially in agreement with the chromoplast differentiation in tomato (Barsan et al, 2012) but shows great differences from the proplastid/ etioplast-to-chloroplast transition in maize/rice (Kleffmann et al, 2007;Majeran et al, 2010). The overall findings from this study provide useful information about chromoplast biogenesis in sweet orange flesh and facilitate a better understanding of its chromoplast differentiation and senescence.…”
Section: Resultssupporting
confidence: 78%
“…Consistent with a previous finding in the chloroplastto-chromoplast transition in tomato (Barsan et al, 2012), the translation machinery of the citrus chromoplast underwent a great loss of efficiency with a strong decrease in abundance of ribosomal proteins of both the chloroplast-encoded small 30S subunit and the large 50S subunit complexes as well as those of the nucleusencoded small 40S subunit and the large 60S subunit during chromoplast differentiation (Fig. 12C).…”
Section: Stability In Protein Import Loss Of Ribosome Assembly and supporting
confidence: 92%
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“…We cannot exclude, however, the possibility that, also in tomato, CLHs may be regulated posttranscriptionally rather than at the expression level. Nevertheless, it is interesting that CLHs have not been identified in proteome analyses of tomato chromoplasts, in contrast to many CCEs, such as PPH, SGR, PAO and RCCR (Barsan et al, , 2012Wang et al, 2013), pointing to their presence, if at all, in rather low abundance.…”
Section: Discussionmentioning
confidence: 99%