Proteomic analysis of glycosomes from Trypanosoma cruzi epimastigotes

Acosta, Héctor; Burchmore, Richard; Naula, Christina; Gualdrón‐López, Melisa; Quintero-Troconis, Ender; Cáceres, Ana J.; Michels, Paul A.M.; Concepción, Juan Luís; Quiñones, Wilfredo

doi:10.1016/j.molbiopara.2019.02.008

Cited by 35 publications

(62 citation statements)

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“…Previously, it has been shown that various enzymes of sterol synthesis in T. brucei and L. major are present in multiple intracellular compartments, including glycosomes (Carrero-Lérida et al, 2009). Various enzymes of the pathway were also detected in our proteomic analysis of T. cruzi glycosomes (Figure 2) (Acosta et al, 2019). Because of the unique aspects of the process in these parasites, several of its enzymes are being considered as a drug target (reviewed in de Souza and Fernandes-Rodrigues, 2009;Buckner and Urbina, 2012).…”

Section: Proteome Of T Cruzi Glycosomesmentioning

confidence: 64%

“…The proteome of T. cruzi glycosomes has recently been determined for the organelles isolated from cultured epimastigotes (Acosta et al, 2019). Many enzymes previously identified in glycosomes of T. brucei (Vertommen et al, 2008;Güther et al, 2014) and Leishmania spp.…”

Section: Proteome Of T Cruzi Glycosomesmentioning

confidence: 99%

“…Interestingly, also detected were enzymes for two possible novel routes for the reoxidation of the glycolytically produced NADH. The first route might involve a D-isomer specific 2-hydroxyacid dehydrogenase (HADH); this enzyme might catalyze the reduction of the pyruvate to lactate, whereas the enzymes for the second route are a putative aldehyde dehydrogenase (ALDH) that could reduce and decarboxylate pyruvate to acetaldehyde and an oxidoreductase (alcohol dehydrogenase, ADH) for the NADH-dependent reduction of acetaldehyde to ethanol (see also Figure 1 in Acosta et al, 2019). However, functional studies to prove these routes remain to be performed.…”

Section: Proteome Of T Cruzi Glycosomesmentioning

confidence: 99%

“…However, functional studies to prove these routes remain to be performed. Further identified, and characterized by our group were several enzymes previously reported to be present in T. cruzi but absent from T. brucei, such as a glucokinase (GlcK), galactokinase (GALK) and a unique form of phosphoglycerate kinase (PAS-PGK) with at its N-terminus a PAS domain, known to possess signaling and regulatory functions Rojas-Pirela et al, 2018;Acosta et al, 2019).…”

Section: Proteome Of T Cruzi Glycosomesmentioning

confidence: 99%

“…Although glycosomes contain an extensive metabolic network, for most pathways only parts are present in the organelles (Figure 2). For glycolysis and gluconeogenesis the majority of the enzymes are compartmentalized, whereas for other pathways, such as the pentose-phosphate metabolism, purine salvage and biosynthesis of pyrimidines and ether lipids only some enzymes were detected in the organelles (Acosta et al, 2019). Moreover, enzymes showed often a dual distribution over glycosomes and cytosol, either due to partial compartmentalization (e.g., Concepción et al, 1999) or represented by distinct isoenzymes (e.g., Barros-Álvarez et al, 2014).…”

Section: Integration Of Glycosomes In Overall T Cruzi Metabolismmentioning

confidence: 99%

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Structure, Properties, and Function of Glycosomes in Trypanosoma cruzi

Quiñones

Acosta

Gonçalves

et al. 2020

Front. Cell. Infect. Microbiol.

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Glycosomes are peroxisome-related organelles that have been identified in kinetoplastids and diplonemids. The hallmark of glycosomes is their harboring of the majority of the glycolytic enzymes. Our biochemical studies and proteome analysis of Trypanosoma cruzi glycosomes have located, in addition to enzymes of the glycolytic pathway, enzymes of several other metabolic processes in the organelles. These analyses revealed many aspects in common with glycosomes from other trypanosomatids as well as features that seem specific for T. cruzi. Their enzyme content indicates that T. cruzi glycosomes are multifunctional organelles, involved in both several catabolic processes such as glycolysis and anabolic ones. Specifically discussed in this minireview are the cross-talk between glycosomal metabolism and metabolic processes occurring in other cell compartments, and the importance of metabolite translocation systems in the glycosomal membrane to enable the coordination between the spatially separated processes. Possible mechanisms for metabolite translocation across the membrane are suggested by proteins identified in the organelle's membrane-homologs of the ABC and MCF transporter families-and the presence of channels as inferred previously from the detection of channel-forming proteins in glycosomal membrane preparations from the related parasite T. brucei. Together, these data provide insight in the way in which different parts of T. cruzi metabolism, although uniquely distributed over different compartments, are integrated and regulated. Moreover, this information reveals opportunities for the development of drugs against Chagas disease caused by these parasites and for which currently no adequate treatment is available.

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