Proteomic analysis of hepatocellular carcinoma HepG2 cells treated with platycodin D

Jj, Lu; Dz, Lu; Chen, Ya-Fang; Dong, Yuchen; Zhang, Junren; T, Li; Zh, Tang; Yang, Zhen

doi:10.1016/s1875-5364(15)30065-0

Cited by 15 publications

(9 citation statements)

References 35 publications

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“…Platycodin D, a triterpenoid saponin isolated from Platycodonis Radix, is a famous Chinese herbal medicine that has been shown to have anti-cancer effects in several cancer cells. The expressions of Galectin-1 (LGALS1) and Keratin, type I cytoskeletal 9 (KRT9) protein were up-regulated in Platycodin D-treated in HepG2 cells 30 . In the present study, JS-K treatment significantly increased LGALS1 and KRT19.…”

Section: Discussionmentioning

confidence: 99%

Identification of targets of JS-K against HBV-positive human hepatocellular carcinoma HepG2.2.15 cells with iTRAQ proteomics

Liu

Zhang

et al. 2021

Sci Rep

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JS-K, a nitric oxide-releasing diazeniumdiolates, is effective against various tumors. We have discovered that JS-K was effective against Hepatitis B virus (HBV)-positive HepG2.2.15 cells. This study used iTRAQ to identify differentially expressed proteins following JS-K treatment of HepG2.2.15 cells. Silenced Transgelin (shTAGLN-2.15) cells were constructed, and the cell viability was analyzed by the CCK8 assay after treatment with JS-K. There were 182 differentially expressed proteins in JS-K treated-HepG2.2.15 cells; 73 proteins were up-regulated and 109 proteins were down-regulated. These proteins were categorized according to GO classification. KEGG enrichment analysis showed that Endocytosis, Phagosome and Proteoglycans were the most significant pathways. RT-PCR confirmed that the expression levels of TAGLN, IGFBP1, SMTN, SERPINE1, ANXA3, TMSB10, LGALS1 and KRT19 were significantly up-regulated, and the expression levels of C5, RBP4, CHKA, SIRT5 and TRIM14 were significantly down-regulated in JS-K treated-HepG2.2.15 cells. Western blotting confirmed the increased levels of USP13 and TAGLN proteins in JS-K treated-HepG2.2.15 cells. Molecular docking revealed the binding of JS-K to TAGLN and shTAGLN-2.15 cells were resistant to JS-K cytotoxicity, suggesting that TAGLN could be an important target in JS-K anti-HBV-positive liver cancer cells. These proteomic findings could shed new insights into mechanisms underlying the effect of JS-K against HBV-related HCC.

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Section: Discussionmentioning

confidence: 99%

Identification of targets of JS-K against HBV-positive human hepatocellular carcinoma HepG2.2.15 cells with iTRAQ proteomics

Liu

Zhang

et al. 2021

Sci Rep

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“…This gene is expressed in various tissues other than the brain, including the lung and pancreas, and several tumor cell lines ( 52 ). KRT9 serves an important role in mature palm and plantar skin tissue and in the morphological processes that form these tissues, and is upregulated ( 53 , 54 ) in platycodin D (PD)-treated HepG2 cells. PLXNB3, a part of the coding family of plexiform proteins, acts as a receptor for signal 5A and serves a role in invasive growth, axon guidance and cell migration.…”

Section: Discussionmentioning

confidence: 99%

Identification of ceRNA network based on a RNA‑seq shows prognostic lncRNA biomarkers in human lung adenocarcinoma

Ran

et al. 2018

Oncol Lett

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Previous studies have emphasized the significant functions of long non-coding RNAs (lncRNAs) as competing endogenous RNAs (ceRNAs) in tumor biology. However, the functions of certain cancer lncRNAs in the lncRNA-related ceRNA network in lung adenocarcinoma (LUAD) are unknown. A systematic and integrative survey of RNA-seq data from The Cancer Genome Atlas (TCGA) was performed to identify candidate lncRNAs for the prognosis of LUAD. In total, 20,502 genes that contain 181 lncRNAs were evaluated in a cohort of 570 LUAD cases. Initially, 6,280 differentially expressed genes (fold-change >2, P<0.05) were obtained using R package, which includes 75 lncRNAs. Next, by univariate regression and multivariate Cox proportional hazards analysis, 32 genes were associated with survival in LUAD. Using these 29 mRNAs and 3 lncRNAs, a prognosis index (PI) was calculated to accurately estimate the survival in LUAD: PI=∑exprisk gene × HRrisk gene. Furthermore, the 32-gene signature was an independent prognostic indicator for LUAD (HR >1; P<0.05, by multivariate analysis). Weighted gene co-expression network analysis (WGCNA) of three risk lncRNAs-FAM138B, NHEG1 and TLX1NB-was performed, based on the P-values of the associated genes, and the top 27 miRNAs that bound to these lncRNAs were predicted by Miranda as target miRNAs. Next, these target miRNAs were transferred to the TarBase, miRTarBase, miRecards and starBase v2.0 databases to obtain their target genes. According to the previous miRNA-mRNA and miRNA-lncRNA data, three lncRNA-miRNA-mRNA ceRNA networks were established, based on the 29 prognostic mRNAs, forming a regulatory network in LUAD. The present study provided insight into the lncRNA-related ceRNA network in LUAD and has identified potential diagnostic and prognostic biomarkers.

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“…Moreover, the combination of platycodin D with clinical medication, such as doxorubicin [207], can significantly enhance the antineoplastic efficacy of the latter. Proteomic analysis has shown that platycodin D can regulate the expression of 19 proteins in HepG2 cells [208]. These findings suggested that saponins demonstrate promising properties for antineoplastic drug development.…”

Section: Discussionmentioning

confidence: 99%

Saponins from Chinese Medicines as Anticancer Agents

Fong

et al. 2016

Molecules

Self Cite

140

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Saponins are glycosides with triterpenoid or spirostane aglycones that demonstrate various pharmacological effects against mammalian diseases. To promote the research and development of anticancer agents from saponins, this review focuses on the anticancer properties of several typical naturally derived triterpenoid saponins (ginsenosides and saikosaponins) and steroid saponins (dioscin, polyphyllin, and timosaponin) isolated from Chinese medicines. These saponins exhibit in vitro and in vivo anticancer effects, such as anti-proliferation, anti-metastasis, anti-angiogenesis, anti-multidrug resistance, and autophagy regulation actions. In addition, related signaling pathways and target proteins involved in the anticancer effects of saponins are also summarized in this work.

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Proteomic analysis of hepatocellular carcinoma HepG2 cells treated with platycodin D

Cited by 15 publications

References 35 publications

Identification of targets of JS-K against HBV-positive human hepatocellular carcinoma HepG2.2.15 cells with iTRAQ proteomics

Identification of targets of JS-K against HBV-positive human hepatocellular carcinoma HepG2.2.15 cells with iTRAQ proteomics

Identification of ceRNA network based on a RNA‑seq shows prognostic lncRNA biomarkers in human lung adenocarcinoma

Saponins from Chinese Medicines as Anticancer Agents

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