Proteomic Analysis of Human Whole and Parotid Salivas Following Stimulation by Different Tastes

Neyraud, Eric; Sayd, Thierry; Morzel, Martine; Dransfield, Eric

doi:10.1021/pr060189z

Cited by 104 publications

(78 citation statements)

References 40 publications

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“…This implies that salivary response is adaptive, since the nature of the response is modulated by the "harmful" nature of the stimulus. This is supported by earlier finding showing that whole saliva proteome can be modified by the nature of the tastant with an overexpression of proteins involved in inflammatory response after stimulation by sour, bitter, and umami tastants but not after sweet (Neyraud et al 2006). Others reported a possible specific response of parotid saliva to tastant by a different protein pattern expression (Dawes 1984) or an increase of α-amylase concentration after drinking sugar solution but not after sham drinking, suggesting a metabolic adaptation of the parotid glands and their specific participation in the digestion and regulation of appetite (Harthoorn et al 2008).…”

Section: Introductionsupporting

confidence: 83%

“…Indeed, it has been reported that during perception of acid, the trigeminal free nerve ending are also stimulated (Lugaz et al 2005). Recently, an overexpression of protein secretion in whole saliva has been found after stimulation by tastants with the strongest modification of the whole saliva proteome after stimulation by acid (Neyraud et al 2006). The apparent oversecretion of protein in parotid saliva after stimulation by acid can be due to a synergic participation of the gustatory and trigeminal system.…”

Section: Taste and Proteinsmentioning

confidence: 99%

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Effects of Different Tastants on Parotid Saliva Flow and Composition

et al. 2009

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Saliva from parotid glands plays a role in taste perception. Parotid saliva is also stimulated by tastants. The aim of this work is to investigate the effects of different tastants on the parotid salivary response in six subjects. Five tastants were given in different concentrations in solution and held in the mouth for 10 s. The flow rate, protein concentration, and pH of secreted parotid saliva were monitored continuously for 5 min. Stimulation by tastants on flow rate response consists of an immediate rise in flow followed by a plateau and a rapid return to prestimulus flow. Response of pH results in a slower increase while protein concentration consists in a slower decrease, both followed by a return to prestimulus levels in about 4 min. From a resting flow rate of about 140μL/min, an increase in flow rate to 370μL/min was caused by stimulation for 10 s with 10 mL of solutions of 0.01 M citric acid, 0.13 M MgSO 4 , 0.25 M monosodium glutamate, 0.5 M NaCl, or 0.5 M sucrose. Comparisons of the different tastants showed that the pH of stimulated parotid saliva increased linearly (r=0.9), irrespective of the nature of the tastant. Protein concentration decreased (r=−0.45) and protein amount increases (r=0.58) with increase in flow rate for all tastants. Corrected for the effects of flow rate, protein amount depended on the nature of the tastant with the greatest secretion after stimulation by citric acid. Flow rate was largely responsible for pH but tastant appears to play an additional role with flow rate on protein secretion.

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Section: Introductionsupporting

confidence: 83%

Section: Taste and Proteinsmentioning

confidence: 99%

Effects of Different Tastants on Parotid Saliva Flow and Composition

et al. 2009

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“…In 2DE profiles, the expression of some protein spots were observed to be differently changed following taste stimulation, these differences being dependent on the type of taste stimuli [84]. This suggests different adaptations of saliva to the five basic tastes.…”

Section: The Use Of Sds Page and 2-de In Salivary Proteome Study Relamentioning

confidence: 97%

The Use of Electrophoresis for the Study of Saliva Involvement in Ingestive Behavior

Lamy¹,

Morzel²,

Rodrigues³

et al. 2015

Field Effect Electroosmosis - A Novel Phenomenon in Electrokinetics and Its Applications in Capillary Electrophoresis

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“…Neyraud 26 analyzed both whole and parotid saliva composition following stimulation of the subjects with different tastes (sweet, bland, sour, acid) using trypsin digests and MALDI analyses. They discovered that two known proteins, calgranulin A and Annexin A1 were nearly absent in parotid saliva, indicating that they most likely originate from other oral tissues.…”

Section: Ferdinandy and Schulzmentioning

confidence: 99%

Radiation Biomarker Research Using Mass Spectrometry

Bach¹,

Hubert²

2007

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S)Air Force Materiel Command AFRL/RHDR Air Force Research Laboratory Human Effectiveness Directorate Information Radiofrequency Radiation Branch SPONSOR/MONITOR'S REPORT Hawks Road NUMBER(S)Brooks City-Base, TX 78235-5147 AFRL-RH-BR-TR-2007-0067 DISTRIBUTION / AVAILABILITY STATEMENTApproved for public release: distribution unlimited. PA #07-332, 15 Oct 2007 SUPPLEMENTARY NOTES Distribution Unlimited (A)14. ABSTRACT Using mass spectrometry to investigate biomarkers from radiation exposure is fairly new. The implementation of mass spectrometric techniques to systems of biological interest has only recently taken off. Therefore, there have only been a limited number of applications of mass spectrometry in radiation dosimetry research. This review is intended to give an overview of mass spectrometry and its application to biological systems and biomarker discovery and how that might relate to relevant radiation dosimetry studies and how these two areas might be combined to benefit both areas of research. Efficient proteomics analyses have necessitated the use of various methods of mass spectrometry in recent years; especially concerning biomarker detection and characterization. A brief summary of the research thus far conducted follows. ........................................................................................................ SUBJECT TERMS AbstractOver 250 references are listed on SciFinder Scholar when searched for radiation biomarkers.1 Less than 5% of those articles deal with mass spectrometry. This indicates an important area of research that still needs to be explored Using mass spectrometry to investigate biomarkers from radiation exposure is fairly new. The implementation of mass spectrometric techniques to systems of biological interest has only recently taken off. Therefore, there have only been a limited number of applications of mass spectrometry in radiation dosimetry research. This review is intended to give an overview of mass spectrometry and its application to biological systems and biomarker discovery and how that might relate to relevant radiation dosimetry studies and how these two areas might be combined to benefit both areas of research.

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Proteomic Analysis of Human Whole and Parotid Salivas Following Stimulation by Different Tastes

Cited by 104 publications

References 40 publications

Effects of Different Tastants on Parotid Saliva Flow and Composition

Effects of Different Tastants on Parotid Saliva Flow and Composition

The Use of Electrophoresis for the Study of Saliva Involvement in Ingestive Behavior

Radiation Biomarker Research Using Mass Spectrometry

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