Proteomic analysis of oxidized proteins in the brain and liver of the Nile tilapia (Oreochromis niloticus) exposed to a water-accommodated fraction of Maya crude oil

Salazar‐Coria, L.; Rocha-Gómez, Maria Alejandra; Matadamas-Martínez, Félix; Yépez‐Mulia, Lilián; Vega-López, Armando

doi:10.1016/j.ecoenv.2019.01.033

Cited by 10 publications

(4 citation statements)

References 117 publications

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“…SOD, CAT, and GPX can be used as "guards" protecting the organisms from the destruction of free radicals [61][62][63][64]. In the present study, the antioxidant enzyme activities were reduced by a low or moderate content of VE (105-152 mg/kg), but were increased as VE content reached levels equal to or above 235 mg/kg.…”

Section: Discussionsupporting

confidence: 42%

An Investigation on the Effects of Dietary Vitamin E on Juvenile Sea Urchin (Strongylocentrotus intermedius): Growth, Intestinal Microbiota, Immune Response, and Related Gene Expression

Li,

Gou,

Gong

et al. 2023

Biology

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A 90 d feeding experiment was conducted to investigate the effects of vitamin E (VE) on growth, intestinal microbiota, immune response, and related gene expression of juvenile sea urchin (Strongylocentrotus intermedius). Six dry feeds were made to contain graded levels of VE (78, 105, 152, 235, 302, and 390 mg/kg); these were named E78, E105, E152, E235, E302, and E390, respectively. Dry feed E50 and fresh kelp (HD) were used as the control diets. There were six replicates of cages in each dietary group, and each cage held 20 sea urchins with an initial body weight of approximately 1.50 g. Results exhibited that weight gain rate and gonadosomatic index (GSI) of the sea urchins were not significantly affected by dietary VE ranging from 78 to 390 mg/kg. Sea urchins in the dry feed groups showed poorer growth performance, but significantly higher GSI than those in the fresh kelp groups. The pepsin and lipase activities were not significantly promoted by low or moderate VE, but were inhibited by a high level of VE (302–390 mg/kg), while amylase and cellulase activities were significantly increased by low or moderate VE, with the highest values observed in the E105 and E235 groups, respectively. VE addition at a low dosage (105–152 mg/kg) showed inhibitory effects on immune and antioxidant enzyme activities and expression of inflammation-related genes, but showed no beneficial effects at moderate or high dosage (235–390 mg/kg), while a moderate or relatively higher level of VE (235–302 mg/kg) significantly increased the expression of several immune-related genes. The relative abundance of Proteobacteria, Actinobacteria, Ruegeria, and Maliponia in the intestine of the sea urchins increased with the increase in VE in the dry feeds. On the contrary, the relative abundance of the Firmicutes, Bacteroidetes, Escherichia-Shigella, Bacteroides, and Clostridium sensu stricto 1 gradually decreased as VE content increased. These results indicated that a moderate level of VE (172.5–262.4) can achieve ideal digestive enzyme activities and growth performance, but a relatively higher level of VE (235–302 mg/kg) was beneficial for maintaining the immune and antioxidant capacity of juvenile S. intermedius by regulating the expression of inflammation- and immune-related genes and abundance of some bacteria to a healthy state.

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Section: Discussionsupporting

confidence: 42%

An Investigation on the Effects of Dietary Vitamin E on Juvenile Sea Urchin (Strongylocentrotus intermedius): Growth, Intestinal Microbiota, Immune Response, and Related Gene Expression

Li,

Gou,

Gong

et al. 2023

Biology

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“…Due to these advantages, NMR-based metabolomics has been extensively applied in ecotoxicology (Brandão et al, 2015;Cappello et al, 2016a, b). Proteomics may provide the significant discrepancies between contaminant-stressed and control conditions, which offer us a direct interpretation of toxicity mechanism after pollution exposure to organisms (Nicholson and Lindon, 2008;Salazar-Coria et al, 2019). The combination of metabolomics and proteomics can provide system-level snapshots of the metabolism of a cell or organism under the stress of toxicants (Chen et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Toxicological effects of As (V) in juvenile rockfish Sebastes schlegelii by a combined metabolomic and proteomic approach

et al. 2019

Environmental Pollution

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Arsenic (As) is a metalloid element that is ubiquitous in the marine environment and its contamination has received worldwide attention due to its potential toxicity. Arsenic can induce multiple adverse effects, such as lipid metabolism disorder, immune system dysfunction, oxidative stress and carcinogenesis, in animals. Inorganic arsenic includes two chemical forms, arsenite (As (III)) and arsenate (As (V)), in natural environment. As (V) is the dominant form in natural waters. In the present study, metabolomic and proteomic alterations were investigated in juvenile rockfish Sebastes schlegelii exposed to environmentally relevant concentrations of As (V) for 14 d. The analysis of iTRAQ-based proteomics combined with untargeted NMR-based metabolomics indicated apparent toxicological effects induced by As (V) in juvenile rockfish. In details, the metabolites, including lactate, alanine, ATP, inosine and phosphocholine were significantly altered in As-treated groups. Proteomic responses suggested that As (V) could not only affected energy and primary metabolisms and signal transduction, but also influenced cytoskeleton structure in juvenile rockfish. This work suggested that the combined proteomic and metabolomic approach could shed light on the toxicological effects of pollutants in rockfish S. schlegelii.

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“…This was consistent with the findings of Song et al [22] who showed that hepatic inflammatory signaling pathway was activated when the fish was subject to oxidative stress. Antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), can be increased and protect the organisms from oxidation stress [41][42][43][44]. In this study, antioxidant enzyme activities in the coelomic fluid were reduced at a low or moderate level, while they were increased by high oxidization (>150 meq/kg).…”

Section: Discussionmentioning

confidence: 78%

Effects of Oxidized Fish Oil on the Growth, Immune and Antioxidant Capacity, Inflammation-Related Gene Expression, and Intestinal Microbiota Composition of Juvenile Sea Urchin (Strongylocentrotus intermedius)

Tang

Heqiu

et al. 2022

Aquaculture Nutrition

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A 60 d feeding experiment was conducted to investigate the effects of oxidized fish oil (OF) on survival, growth, immune and antioxidant capacity, inflammation-related gene expression, and intestinal microbiota of juvenile sea urchin (Strongylocentrotus intermedius). Six feeds were formulated by including equal amounts of fish oil with different oxidation levels (0, 50, 100, 150, 200, and 300 meq/kg). The feed OF0 and fresh kelp (Laminaria japonica) were used as the control groups. Results showed that the weight gain rate (WGR) of sea urchins significantly decreased as the oxidation level increased. When the oxidation level of fish oil was equal to or above 150 meq/kg, the WGR of sea urchins was significantly lower than those fed the control diets. The activities of digestive enzymes (pepsin and amylase), immune enzymes (alkaline phosphatase and acid phosphatase), and antioxidant enzymes (superoxide dismutase, catalase, and glutathione S-transferase) first decreased significantly as the oxidation level increased to 150 or 200 meq/kg and then bounced to values comparable to or even higher than those in the control feed group, while the transcription of immune, antioxidation, and inflammation-related genes was upregulated by the increasing oxidation level of fish oil. Oxidized fish oil decreased the intestinal microbial diversity and the relative abundance of Firmicutes. Vibrio was the dominant genus of microbiota in the intestine of sea urchins fed almost all formulated feeds (except for OF150), with its abundance above 20%. Comparably, the abundance of Vibrio in the intestine of sea urchins fed fresh kelp was only 2.32%. These results showed that oxidized fish oil had negative effects on the growth, immune response and antioxidant capacity, and bacterial diversity of juvenile S. intermedius. The abnormal increase of antioxidant enzyme activities and malondialdehyde contents at relatively higher oxidation level (>150 meq/kg) could be due to the oxidative stress occurrence inside the body of sea urchins. Sea urchins fed formulated feeds host more Vibrio than those fed fresh kelp, and this could be a potential incidence of disease occurrence.

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Proteomic analysis of oxidized proteins in the brain and liver of the Nile tilapia (Oreochromis niloticus) exposed to a water-accommodated fraction of Maya crude oil

Cited by 10 publications

References 117 publications

An Investigation on the Effects of Dietary Vitamin E on Juvenile Sea Urchin (Strongylocentrotus intermedius): Growth, Intestinal Microbiota, Immune Response, and Related Gene Expression

An Investigation on the Effects of Dietary Vitamin E on Juvenile Sea Urchin (Strongylocentrotus intermedius): Growth, Intestinal Microbiota, Immune Response, and Related Gene Expression

Toxicological effects of As (V) in juvenile rockfish Sebastes schlegelii by a combined metabolomic and proteomic approach

Effects of Oxidized Fish Oil on the Growth, Immune and Antioxidant Capacity, Inflammation-Related Gene Expression, and Intestinal Microbiota Composition of Juvenile Sea Urchin (Strongylocentrotus intermedius)

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