Proteomic analysis of peripheral layers during wheat (Triticum aestivum L.) grain development

Abstract

Grains of hexaploid wheat, Triticum aestivum (cv. Récital), were collected at 15 stages of development, from anthesis to physiological maturity, 0-700°C days (degree days after anthesis). Two hundred and seven proteins of grain peripheral layers (inner pericarp, hyaline, testa and aleurone layer) were identified by 2-DE, MALDI-TOF MS and data mining, then were classified in 16 different functional categories. Study of the protein expression over time allowed identification of five main profiles and four distinct phases of development. Composite expression curves indicated that there was a shift from metabolic processes, translation, transcription and ATP interconversion towards storage and defence processes. Protein synthesis, protein turnover, signal transduction, membrane transport and biosynthesis of secondary metabolites were the mediating functions of this shift. A picture of the dynamic processes taking place in peripheral layers during grain development was obtained in this study. It should further help in the construction of proteome reference maps for the developing peripheral layers.

New insights into the effects of high temperature, drought and post-anthesis fertilizer on wheat grain development

“…Mak et al (2006) identified 347 proteins in wheat germ, while Jerkovic et al (2010) focused on proteins in the peripheral layers of the grain and identified proteins in 35 spots from the epidermis and hypodermis, 119 spots from cross cells, tube cells, testa and nucellar tissue and 672 spots from aleurone. Several studies also determined the accumulation profiles of proteins throughout development in endosperm , peripheral grain layers (Tasleem-Tahir et al, 2011) or whole grain (Gao et al, 2009;Nadaud et al, 2010). These studies provide insights into molecular processes that take place in various parts of the grain during wheat grain development and establish a detailed picture of flour protein composition that is critical for understanding flour quality.…”

Expression Profiling of Starchy Endosperm Metabolic Proteins at 21 Stages of Wheat Grain Development

“…The spots were destained and digested using the method described previously. 12 For LC−MS/MS analysis, peptide mixtures were analyzed by online nanoflow liquid chromatography using the Ultimate 3000 RSLC (Dionex, Voisins le Bretonneux, France) with nanocapillary columns of 15 cm length × 75 μm i.d (Acclaim Pep Map RSLC, Dionex). The solvent gradient increased linearly from 4 to 50% ACN in 0.5% formic acid at a flow rate of 300 nL/min for 30 min.…”

Proteomic analysis of maize grain development using iTRAQ reveals temporal programs of diverse metabolic processes

“…Such studies have been performed in many species, including rice [15, 18, 19], wheat [20], Arabidopsis [17], barley [21], castor [22], Medicago truncatula [23], and soybean [24, 25]. However, some types of protein can’t be analyzed by 2-DE as it has the inherent restrictions of being unable to separate hydrophobic proteins, low identification rate, and lack of accurate quantitative information [26, 27].…”