Proteomic analysis of the cold stress response in the moss, Physcomitrella patens

Abstract

Cold stress has adverse effects on plant growth and development. Plants respond and acclimate to cold stress through various biochemical and physiological processes, thereby acquiring stress tolerance. To better understand the basis for tolerance, we carried out a proteomic study in the model moss, Physcomitrella patens, characterizing gametophore proteins with 2-DE and mass spectroscopy. Following exposure to 0 degrees C for up to 3 days, out of the more than 1000 protein spots reproducibly resolved, only 45 changed in abundance by at least 1.5-fold. Of these, 35 were identified by tryptic digestion and mass spectroscopy. Photosynthetic proteins decreased, whereas many catabolic proteins increased. In addition, cold stress up-regulated a variety of signaling, cytoskeleton, and defense proteins and few proteins in these classes were down-regulated. Up-regulated proteins include the 14-3-3-like protein, actin, HSP70s, lipoxygenases, and cytochrome P450 proteins. These results point to pathways that are important for the mechanism of cold stress response in P. patens and by extension to the entire plant kingdom.

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

“…Under stress, homoiochlorophyllous plants protect photosystem proteins from degradation due to destruction of chloroplasts (Georgieva et al, 2007). A number of proteomic studies on the moss reaction to drought, high salinity, and low temperature were conducted with use of 2D-electrophoresis (Wang et al, 2008, 2009). It was shown that under drought or salinity, the abundance of light-harvesting proteins increased and the ATP-synthase subunits were down-regulated, but in general there was no significant damage to the photosynthetic apparatus.…”

“…It was shown that under drought or salinity, the abundance of light-harvesting proteins increased and the ATP-synthase subunits were down-regulated, but in general there was no significant damage to the photosynthetic apparatus. At low temperature, the photosystem and light-harvesting proteins were down-regulated and the dark-phase proteins changed bi-directionally (Wang et al, 2009). …”

“…This is consistent with the data for other salt-tolerant organisms (Wang et al, 2013). Under low-temperature stress, the moss down-regulates photosynthetic protein abundance and up-regulates stress-related and some Calvin cycle proteins (Wang et al, 2009). …”

Contrasting cDNA-AFLP profiles between crown and leaf tissues of cold-acclimated wheat plants indicate differing regulatory circuitries for low temperature tolerance

“…Using cDNA microarray experiments in Arabidopsis, Narusaka et al (2004) showed abiotic and biotic stress response crosstalk in the expression of cytochrome P450 gene superfamily. In a moss, Physcomitrella patens, cold stress led to up-regulation of cytochrome P450 protein (Wang et al 2009b). Choline-phosphate cytidylyltransferase, also identified in our study, is generally accepted as the rate limiting step in nucleotide pathway of phosphatidyl choline formation, the major component of biological membranes (Vance and Choy 1979).…”

Proteomics in China: Ready for prime time

“…), development stages (seed or tube germination [115,116,[125][126][127]136], fiber elongation [107], fruit senescence [109,137], etc.) and responses to environmental milieu (temperature change [119,[128][129][130], nutritional deficiency or imbalance [102,113], wound [132,133], etc.). To investigate the responses of rice seedlings to H 2 O 2 stress, changes in protein expression were analyzed using a comparative proteomics approach.…”