Proteomic analysis of the interaction of Bifidobacterium longum NCC2705 with the intestine cells Caco-2 and identification of plasminogen receptors

Wei, Xiao; Yan, Xiabei; Chen, Xuannan; Yang, Zhan; Li, Huan; Zou, Dayang; He, Xiaoqing; Wang, Simiao; Cui, Qian; Liu, Wei; Zhurina, Daria; Wang, Xuesong; Zhao, Xiangna; Huang, Liuyu; Zeng, Ming; Ye, Qinong; Riedel, Christian U.; Yuan, Jing

doi:10.1016/j.jprot.2014.04.038

Cited by 26 publications

(23 citation statements)

References 21 publications

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“…For instance, RNAPα and BglA3 possess C-terminal lysine residues. Although C-terminal lysine residue is absent in EFTu, this protein has been identified as a Plg receptor414243. Instead of C-terminal lysines, it seems that EFTu/Plg interaction occur via internal amino acid regions ( 179 LKALEGDSHYEDIV 192 and 249 VGIKEETQKAV 259 ).…”

Section: Discussionmentioning

confidence: 99%

“…Apart from Plg binding, cytoplasm-localized EFTu regulates protein synthesis and cellular metabolism by binding to and transporting amino-acyl-tRNA to the ribosomes confirming its RNA-binding ability44. Since EFTu, similarly to Eno, is associated with the cell wall of various bacteria414243, it is imaginable that other cytosolic proteins such as RNAPα and/or BglA3 may also be transported to the bacterial surface thereby acting as “moonlighting proteins” for eRNA-binding at certain steps of the growth phase.…”

Section: Discussionmentioning

confidence: 99%

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Host-derived extracellular RNA promotes adhesion of Streptococcus pneumoniae to endothelial and epithelial cells

Zakrzewicz

Bergmann

Didiášová

et al. 2016

Sci Rep

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Streptococcus pneumoniae is the most frequent cause of community-acquired pneumonia. The infection process involves bacterial cell surface receptors, which interact with host extracellular matrix components to facilitate colonization and dissemination of bacteria. Here, we investigated the role of host-derived extracellular RNA (eRNA) in the process of pneumococcal alveolar epithelial cell infection. Our study demonstrates that eRNA dose-dependently increased S. pneumoniae invasion of alveolar epithelial cells. Extracellular enolase (Eno), a plasminogen (Plg) receptor, was identified as a novel eRNA-binding protein on S. pneumoniae surface, and six Eno eRNA-binding sites including a C-terminal 15 amino acid motif containing lysine residue 434 were characterized. Although the substitution of lysine 434 for glycine (K434G) markedly diminished the binding of eRNA to Eno, the adherence to and internalization into alveolar epithelial cells of S. pneumoniae strain carrying the C-terminal lysine deletion and the mutation of internal Plg-binding motif were only marginally impaired. Accordingly, using a mass spectrometric approach, we identified seven novel eRNA-binding proteins in pneumococcal cell wall. Given the high number of eRNA-interacting proteins on pneumococci, treatment with RNase1 completely inhibited eRNA-mediated pneumococcal alveolar epithelial cell infection. Our data support further efforts to employ RNAse1 as an antimicrobial agent to combat pneumococcal infectious diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Host-derived extracellular RNA promotes adhesion of Streptococcus pneumoniae to endothelial and epithelial cells

Zakrzewicz

Bergmann

Didiášová

et al. 2016

Sci Rep

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“…The contact of Lactobacillus casei ATCC 334 with human intestinal epithelial cells promotes functional changes in the bacteria, which acquire a more immunosuppressive phenotype as demonstrated by the ability of L. casei to generate functional regulatory T cells (CD4 + CD25 + FoxP3 + ) and production of the anti-inflammatory cytokine IL-10 29 . Cultivation of B. longum NCC2705 with intestinal epithelial cells has been also shown to induce adhesin expression 30 .…”

Section: Interaction Of Probiotics With Host Cells and Commensal Micrmentioning

confidence: 99%

The Role of Probiotics on the Microbiota

et al. 2016

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The microbiota and the human host maintain a symbiotic association. Nowadays, metagenomic analyses are providing valuable knowledge on the diversity and functionality of the gut microbiota. However, with regard to the definition of a "healthy microbiota" and the characterization of the dysbiosis linked to obesity, there is still not a clear answer. Despite this fact, attempts have been made to counteract obesity through probiotic supplementation. A literature search of experimental studies relevant to the topic was performed in PubMed database with the keywords "probiotic" and "obesity" and restricted to those with "Lactobacillus" or "Bifidobacterium" in the title. So far, evidence of an antiobesity effect of different lactobacilli and bifidobacteria has been mainly obtained from animal models of dietary-induced obesity. Using these experimental models, a substantial number of studies have reported reductions in weight gain and, in particular, fat tissue mass at different locations following administration of bacteria, as compared with controls. Antiatherogenic and anti-inflammatory effects-including regulation of expression of lipogenic and lipolytic genes in the liver, reduction in liver steatosis, improvement of blood lipid profile and glucose tolerance, decreased endotoxemia, and regulation of inflammatory pathways-are also reported in many of them. The number of human studies focused on probiotic administration for obesity management is still very scarce, and it is too soon to judge their potential efficacy, especially when considering the fact that the actions of probiotics are always strain specific and the individual response varies according to intrinsic factors, the overall composition of diet, and their interactions.

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“…plantarum [131,132]. Furthermore, surface-exposed or secreted proteomes are often modulated by intestinal factors like co-culture with intestinal cell lines [133], low pH [134,135], bile salts [72,98,136] or mucin [137,138], or co-cultivation in a caecum-like environment [139], thus pointing to intestinal regulation of functions crucial for probiotics intestinal colonization. Indeed, implantation of a B. longum culture into a rabbit intestine confirmed in vivo over-production of elongation factor Tu [103].…”

Section: Proteomics Of Sub-cellular Fractionsmentioning

confidence: 99%

Tackling probiotic and gut microbiota functionality through proteomics

Ruíz

Hidalgo

Blanco-Míguez

et al. 2016

Journal of Proteomics

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Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Molecular basis underlying the functional properties of probiotic bacteria responsible for the health promoting effects have been in the background for many years. Breakthrough of omics technologies in the probiotic and microbiota fields has had a very relevant impact in the elucidation of probiotic mechanisms and in the procedures to select these microorganisms, based on solid scientific evidence. It is unquestionable that, in the near future, the evolution of proteomic techniques will play a pivotal role in the generation of knowledge about the functions of probiotics and gut commensals, still a pending issue in the field of intestinal microbiomics.

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Proteomic analysis of the interaction of Bifidobacterium longum NCC2705 with the intestine cells Caco-2 and identification of plasminogen receptors

Cited by 26 publications

References 21 publications

Host-derived extracellular RNA promotes adhesion of Streptococcus pneumoniae to endothelial and epithelial cells

Host-derived extracellular RNA promotes adhesion of Streptococcus pneumoniae to endothelial and epithelial cells

The Role of Probiotics on the Microbiota

Tackling probiotic and gut microbiota functionality through proteomics

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