Proteomic analysis reveals contrasting stress response to uranium in two nitrogen-fixing Anabaena strains, differentially tolerant to uranium

Panda, Bandita; Basu, Bhakti; Acharya, Celin; Rajaram, Hema; Apte, Shree Kumar

doi:10.1016/j.aquatox.2016.12.002

Cited by 20 publications

(6 citation statements)

References 30 publications

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“…Previous proteomic studies observed that HSP70 was significantly decreased in Arabidopsis exposed to cadmium stress (Sarry et al, 2006). In this study, HSP70 (spot 7006, 7407, 7707, and 8311) and molecular chaperone DnaK (spot 7701) were decreased by copper stress, which was consistent with the change of HSP70 in poplar stressed by cadmium (Kieffer, Dommes, Hoffmann, Hausman, & Renaut, 2008) and DnaK in Anabaena exposed with uranium (Panda, Basu, Acharya, Rajaram, & Apte, 2017). The downregulation of HSP/chaperone discovered that copper ion inhibited efficiently the chaperone-assisted refolding of denatured proteins and destroyed partly the cellular homeostasis.…”

Section: Ta B L E 1 (Continued)supporting

confidence: 88%

Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic

et al. 2018

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Heavy metal pollution in Antarctic is serious by anthropogenic emissions and atmospheric transport. To dissect the heavy metal adaptation mechanisms of sea-ice organisms, a basidiomycetous yeast strain AN5 was isolated and its cellular changes were analyzed. Morphological, physiological, and biochemical characterization indicated that this yeast strain belonged to Rhodotorula mucilaginosa AN5. Heavy metal resistance pattern of Cd > Pb = Mn > Cu > Cr > Hg was observed. Scanning electron microscopic (SEM) results exhibited altered cell surface morphology under the influence of copper metal compared to that with control. The determination of physiological and biochemical changes manifested that progressive copper treatment significantly increased antioxidative reagents content and enzymes activity in the red yeast, which quench the active oxygen species to maintain the intercellular balance of redox state and ensure the cellular fission and growth. Comparative proteomic analysis revealed that, under 2 mM copper stress, 95 protein spots were tested reproducible changes of at least 10-fold in cells. Among 95 protein spots, 43 were elevated and 52 were decreased synthesis. After MALDI TOF MS/MS analysis, 51 differentially expressed proteins were identified successfully and classified into six functional groups, including carbohydrate and energy metabolism, nucleotide and protein metabolism, protein folding, antioxidant system, signaling, and unknown function proteins. Function analysis indicated that carbohydrate and energy metabolism-, nucleotide and protein metabolism-, and protein folding-related proteins played central role to the heavy metal resistance of Antarctic yeast. Generally, the results revealed that the yeast has a great capability to cope with heavy metal stress and activate the physiological and protein mechanisms, which allow more efficient recovery after copper stress. Our studies increase understanding of the molecular resistance mechanism of polar yeast to heavy metal, which will be benefitted for the sea-ice isolates to be a potential candidate for bioremediation of metal-contaminated environments.

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Section: Ta B L E 1 (Continued)supporting

confidence: 88%

Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic

et al. 2018

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“…Note that despite predominance of Burkholderia spp., documented at several uranium contaminated environments, studies have not been performed to assess how these Proteobacterial members respond against uranium stress, which could provide critical clues on uranium bioremediation and recommendations on better stewardship of nuclear materials contaminated ecosystems can be offered. To our knowledge, there are only a few studies available, where different bacterial strains were exposed to U and proteomes were analyzed [19,20,36,42,43].…”

Section: Discussionmentioning

confidence: 99%

Proteogenomic Analysis of Burkholderia Species Strains 25 and 46 Isolated from Uraniferous Soils Reveals Multiple Mechanisms to Cope with Uranium Stress

Agarwal

Pathak

Rathore

et al. 2018

Cells

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Two Burkholderia spp. (strains SRS-25 and SRS-46) were isolated from high concentrations of uranium (U) from the U.S. Department of Energy (DOE)-managed Savannah River Site (SRS). SRS contains soil gradients that remain co-contaminated by heavy metals from previous nuclear weapons production activities. Uranium (U) is one of the dominant contaminants within the SRS impacted soils, which can be microbially transformed into less toxic forms. We established microcosms containing strains SRS-25 and SRS-46 spiked with U and evaluated the microbially-mediated depletion with concomitant genomic and proteomic analysis. Both strains showed a rapid depletion of U; draft genome sequences revealed SRS-25 genome to be of approximately 8,152,324 bp, a G + C content of 66.5, containing a total 7604 coding sequences with 77 total RNA genes. Similarly, strain SRS-46 contained a genome size of 8,587,429 bp with a G + C content of 67.1, 7895 coding sequences, with 73 total RNA genes, respectively. An in-depth, genome-wide comparisons between strains 25, 46 and a previously isolated strain from our research (Burkholderia sp. strain SRS-W-2-2016), revealed a common pool of 3128 genes; many were found to be homologues to previously characterized metal resistance genes (e.g., for cadmium, cobalt, and zinc), as well as for transporter, stress/detoxification, cytochromes, and drug resistance functions. Furthermore, proteomic analysis of strains with or without U stress, revealed the increased expression of 34 proteins from strain SRS-25 and 52 proteins from strain SRS-46; similar to the genomic analyses, many of these proteins have previously been shown to function in stress response, DNA repair, protein biosynthesis and metabolism. Overall, this comparative proteogenomics study confirms the repertoire of metabolic and stress response functions likely rendering the ecological competitiveness to the isolated strains for colonization and survival in the heavy metals contaminated SRS soil habitat.

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“…PCC7120 (hereinafter, Anabaena) was selected as a model organism, given its environmental relevance and ubiquity, as well as the extensive bibliography regarding the genetic, physiology and ecological importance. Furthermore, this cyanobacterium has been used regularly as model for ecotoxicological studies, including the field of nanotoxicology 31,[33][34][35][36][37][38][39][40] .…”

Section: Introductionmentioning

confidence: 99%

Mechanism of the toxic action of cationic G5 and G7 PAMAM dendrimers in the cyanobacteriumAnabaenasp. PCC7120

Tamayo-Belda

González-Pleiter

Pulido-Reyes

et al. 2019

Environ. Sci.: Nano

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Proteomic analysis reveals contrasting stress response to uranium in two nitrogen-fixing Anabaena strains, differentially tolerant to uranium

Cited by 20 publications

References 30 publications

Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic

Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic

Proteogenomic Analysis of Burkholderia Species Strains 25 and 46 Isolated from Uraniferous Soils Reveals Multiple Mechanisms to Cope with Uranium Stress

Mechanism of the toxic action of cationic G5 and G7 PAMAM dendrimers in the cyanobacteriumAnabaenasp. PCC7120

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