Proteomic and metabolomic analysis of minimax and Williams 82 soybeans grown under two different conditions

Abstract

MiniMax was developed as a model soybean for research because of its small stature, limited planting space, and rapid generation time. In the present study, the proteins, isoflavones, and fatty acid methyl esters (FAMEs) were compared between dwarf (MiniMax) and commercially cultivated (Williams 82) soybean genotypes under two growing conditions (greenhouse and field). The protein profile of the soybean extracts by 2D‐PAGE showed at least 16 of 716 spots that were differently expressed in MiniMax and Williams 82. These spots were primarily six proteins namely, seed biotin, β‐conglycinin, glycinin, seed‐maturation protein, glutathione‐S‐transferase, and sucrose binding protein. In both genotypes, higher content of isoflavone aglycones was observed in greenhouse grown samples as compared to the field grown samples. However, only field grown Willams 82 had higher levels of FAMEs as compared to other three groups. This study shows that both growing conditions and cultivars significantly influence the compositional content of metabolites. Practical applications The global demand of soybean and its products has increased due to its high protein and isoflavones content. Researchers are actively developing new genotypes with improved yields, greater drought and disease tolerance, and reduced growth time. MiniMax, a dwarf genotype of soybean showed early flowering, seed setting, and maturation traits due to its multiple dwarfing genes. This characteristic feature enables researchers to study effects of various treatments on soybean in a shorter period with multiple replications in a single growing season.

Effect of processing methods on the content of phenolic compounds in Vicia faba L. tissues grown in field and greenhouse

“…In our study, the amounts of TP and TF were higher in field plants mostly with oven-dried samples than in those grown in the greenhouse, a finding that differs from that of John et al (2016) in which different varieties of soybean grown in a greenhouse (22.2 °C; photoperiod of up to 16 h) produced higher yields of isoflavones (daidzin, genistin, glycitein, daidzein and genistein) than plants grown in the field (21.7 °C; natural photoperiod). Moreover, when soybeans were cultivated in the field and the greenhouse under similar conditions of temperature and photoperiod, the accumulation of proteins and isoflavones remained higher in the greenhouse plants (John et al, 2017). The discrepancy between these results and those presented herein may be explained by differences in species and tissues analyzed and/or by differences in growing conditions in the field and in the greenhouse that prevailed in our study.…”

“…A number of studies have shown that growing conditions (field or greenhouse) exert significant effects on the content of primary and secondary metabolites in soybean seeds (John et al, 2016(John et al, , 2017. In our study, the amounts of TP and TF were higher in field plants mostly with oven-dried samples than in those grown in the greenhouse, a finding that differs from that of John et al (2016) in which different varieties of soybean grown in a greenhouse (22.2 °C; photoperiod of up to 16 h) produced higher yields of isoflavones (daidzin, genistin, glycitein, daidzein and genistein) than plants grown in the field (21.7 °C; natural photoperiod).…”

Seed Development and Protein Accumulation Patterns in Faba Bean (Vicia faba, L.)

“…This reduction was accompanied by downregulation of the expression of two legumin genes during seed development. In addition, in soybean, the abundance of several seed storage proteins was reported to vary considerably depending on whether genotypes were grown in the field or a glasshouse, indicating changes in protein composition due to prevailing conditions during seed growth. Therefore, better characterization of seed development and accumulation patterns of different storage proteins is the first step toward the rational modification of seed protein composition through genetics or agronomic practices.…”

Metabolomics as a Prospective Tool for Soybean (Glycine max) Crop Improvement

“…The interaction of these pressures will influence the physiological response of plants. Furthermore, different organs or cultivars of soybean plants respond differently to the same stress, and the plant’s metabolic system is a constantly changing network of interconnections [ 2 , 96 , 97 ]. Nevertheless, the study of the soybean metabolome paves the way to a better understanding of complex metabolic pathways and stress-associated metabolites.…”