Proteomic and metabolomic analysis on the toxicological effects of As (III) and As (V) in juvenile mussel Mytilus galloprovincialis

Abstract

h i g h l i g h t s g r a p h i c a l a b s t r a c tAs (III) and As (V) induced differential proteomic responses in mussels. As (III) and As (V) induced osmotic and immune stresses in mussels. As (V) disturbed energy metabolism at metabolite level in mussels.a r t i c l e i n f o a b s t r a c tInorganic arsenic (As) is a known pollutant including two chemical forms (arsenite (As III) and arsenate (As V)), in marine and coastal environment. Marine mussel Mytilus galloprovincialis is an important environmental monitoring species around the world. In this study, we focused on valence-specific responses of As in juvenile mussel M. galloprovincialis using a combined proteomic and metabolomic approach. Metabolic responses indicated that As (III) mainly caused disturbance in osmotic regulation in juvenile mussels. As (V) caused disturbances in both osmotic regulation and energy metabolism marked by different metabolic responses, including betaine, taurine, glucose and glycogen. Proteomic responses exhibited that As (III) had a significant negative effect on cytoskeleton and cell structure (actin and collagen alpha-6(VI) chain). As (V) affected some key enzymes involved in energy metabolism (cytosolic malate dehydrogenase, cMDH) and cell development (ornithine aminotransferase and astacin). Overall, all these results confirmed the valence-specific responses in juvenile mussels to As exposures. These findings demonstrate that a combined metabolomic and proteomic approach could provide an important insight into the toxicological effects of environmental pollutants in organisms.

The impacts of As accumulation under different pH levels: Comparing Ruditapes decussatus and Ruditapes philippinarum biochemical performance

“…energy metabolism) but also on their biochemical performance (e.g. detoxification mechanisms) (Diniz et al, 2008;Chenglong et al, 2013;Freitas et al, 2016;Rajkumar and Rajkumar, 2013;Vernberg, 2012;Velez et al, , 2016bWu et al, 2013a;Yu et al, 2016).…”

Toxicological effects of As (V) in juvenile rockfish Sebastes schlegelii by a combined metabolomic and proteomic approach

“…The potential toxicity of As has received worldwide attention due to its frequent detection in the environment. As can stimulate numerous molecular events in connection with lipid metabolism disorder, immune system dysfunction, oxidative stress and carcinogenesis in organisms, which have been well documented in a variety of organisms (Chen et al, 2018a,b;Chen et al, 2019;Ramsey et al, 2013;Szymkowicz et al, 2019;Xu et al, 2013;Yu et al, 2016). Since researchers often take advantage of traditional toxicological approaches to focus on a known class of toxicity-responsive molecules to draw out the toxic mechanisms, it is reluctant to discover new biological molecules associated with toxicological effects and presents initial and less comprehensive evaluations on toxicological reactions of pollutants in organisms (Ji et al, 2014).…”

Changes in protein expression in mussels Mytilus galloprovincialis dietarily exposed to PVP/PEI coated silver nanoparticles at different seasons

“…Cytosolic malate dehydrogenase is an enzyme that plays an important role in energy metabolism including the malate-aspartate (or NADH) shuttle, the acetate shuttle in lipogenesis and gluconeogenesis (Dahlhoff and Somero, 1993;Fields et al, 2006). Alteration in the expression of this protein has been already reported in juvenile mussels as well as in adult mussels M. galloprovincialis after the exposure to arsenate for 48 hours (Yu et al, 2016) and under acute heat stress (Tomanek and Zuzow, 2010), respectively, which was associated with the disturbance of energy metabolism in both cases. Thus, the exposure of mussels to Ag NPs through the diet seem to alter the energy metabolism in mussels digestive gland in autumn.…”