Proteomic approach to study the cytotoxicity of dioscin (saponin)

Abstract

Dioscin, extracted from the root of Polygonatum zanlanscianense pamp, exhibits cytotoxicity towards human myeloblast leukemia HL-60 cells. Proteomic analysis revealed that the expression of mitochondrial associated proteins was substantially altered in HL-60 cells corresponding to the dioscin treatment, suggesting that mitochondria are the major cellular target of dioscin. Mitochondrial functional studies validated that mitochondrial apoptotic pathway was initiated by dioscin treatment. Changes in proteome other than mitochondrial related proteins implicate that other mechanisms were also involved in dioscin-induced apoptosis in HL-60 cells, including the activity impairment in protein synthesis, alterations of phosphatases in cell signaling, and deregulation of oxidative stress and cell proliferation. Current study of protein alterations in dioscin-treated HL-60 cells suggested that dioscin exerts cytotoxicity through multiple apoptosis-inducing pathways.

Timosaponin A-III Induces Autophagy Preceding Mitochondria-Mediated Apoptosis in HeLa Cancer Cells

“…1A). Sarsasapogenin does not have the double bond at C 5 and C 6 and structurally resembles the aglycones of dioscin and polyphyllin D, which were previously reported to exhibit anticancer activities (1,2). TAIII was shown to suppress arachidonic acid-induced superoxide generation in human neutrophils (4) and increase the intracellular Ca 2+ concentration in endothelial cells (5).…”

“…Evidence is accumulating to prove that saponins can inhibit tumor cell proliferation, with great potential to be developed as chemotherapeutic agents (1,2). Anemarrhena asphodeloides Bge.…”

Separation and identification of Musa acuminate Colla (banana) leaf proteins by two-dimensional gel electrophoresis and mass spectrometry

“…0.5 mL Eppendorf tubes. The samples were digested following the protocol described by Wang Ying with some modifications (Wang et al, 2006). Briefly, the spots were washed twice with distilled water and destained with 15 mM potassium ferricyanide and 50 mM sodium thiosulfate and dehydrated in acetonitrile (ACN) followed by 25 mM ammonium bicarbonate.…”

Immuno-Modulatory Effects of Phytomedicines Evaluated Using Omics Approaches

“…Recent biological and pharmaceutical researches have shown that diosgenyl saponins may exert a large variety of biological functions, with a potential for use in cancer chemoprevention [88]. By using 2-DE, tryptic in-gel digestion and MALDI-TOF MS analysis, Wang et al [89] suggested that dioscin, a saponin extracted from Polygonatum zanlanscianense Pamp., exhibited cytotoxicity towards human myeloblast leukemia HL-60 cells. This proteomics analysis also revealed that the expression of mitochondria-associated proteins was substantially altered in HL-60 cells upon dioscin treatment, suggesting that mitochondria were the major cellular and organelle target of dioscin cytotoxicity.…”