Proteomic Functional Characterization of Bovine Stromal Vascular Cells from Omental, Subcutaneous and Intramuscular Adipose Depots

Abstract

Anatomically separate fat depots differ in size, function, and contribution to pathological states such as the metabolic syndrome. We isolated pre-adipocytes from different adipose depots, omental, subcutaneous and intramuscular, of beef cattle, and cultured in vitro to determine the basis for the variations and attribute these variations to the inherent properties of adipocyte progenitors. The proliferating cells from all depots before the confluence were harvested and the proteome was analyzed by a functional proteomic approach, involving 2-DE and MALDI-TOF/TOF. More than 252 protein spots were identified, selected and analyzed by Image Master (ver 7.0) and MALDI-TOF/TOF. Further, our analysis showed that there were specific differences in proteome expression patterns among proliferating precursor cells from the three depots. Sixteen proteins were found to be differentially expressed and these were identified as proteins involved in cellular processes, heat shock/chaperones, redox proteins, cytoskeletal proteins and metabolic enzymes. The results also enabled us to understand the basic roles of these proteins in different inherent properties exhibited by adipose tissue depots.

The effect of single-nucleotide polymorphisms within heat shock protein beta 1 on beef quantity in Korean native steers

“…The cellular development associated with adipose tissue growth involves both cellular hypertrophy (increase in size) and hyperplasia (increase in number). Rajesh et al (2010) reported that HSPB1 interact with insulin-like growth factor receptor 1 and its signal transducer, the serine/threonine kinase Akt, which together modulate an adipocyte metabolism. Specifically, the previous results suggested that HSPB1 was negatively correlated with an adipose metabolism (Kim et al, 2011).…”