The effect of single-nucleotide polymorphisms within heat shock protein beta 1 on beef quantity in Korean native steers

Abstract

Abstract. Heat shock protein beta 1 (HSPB1), a member of the heat-shock family of protein, is a relatively small (27 kDa) molecular chaperone protein associated with cellular development. The relationship between HSPB1 expression and muscle growth in beef cattle has previously been reported, but there have been no reports of DNA markers related to meat quantity in Korean native steers. Therefore, the aim of this study was to evaluate the relationship of single-nucleotide polymorphisms (SNPs) within HSPB1 in terms of the carcass traits related to muscle growth in Korean native steers. Through direct sequencing, we discovered three SNPs: g.111 T > C SNP (rs208395876) and g.2548 C > G SNP (rs483014585) were respectively located in 5′ UTR (untranslated region) and 3′ UTR. Further, g.2352 T > C SNP (rs110832311) was located in the adjacent region of the RNA splicing site. The least square means of steers with a CC genotype of g.111 T > C SNP had a significantly higher meat ratio (P = 0.04), while the least square means of steers with a CC genotype of g.2352 T > C SNP had a significantly higher meat ratio (P = 0.002) and lower back-fat thickness (P = 0.004) than those of the other genotype. Moreover, although the least square means of steers with CC-CC, CT-CC, and TT-CC genotypes were significantly decreased for back-fat thickness, they were significantly increased for the meat ratio. Therefore, our results suggested that g.111 T > C SNP and g.2352 T > C SNP could be a causal mutation related to an adipose metabolism in Korean cattle steer.

Allele-specific polymerase chain reaction for the discrimination of elite Korean cattle associated with high beef quality and quantity

“…The SNPs were related to the production of high-quantity beef in Hanwoo. The g.2352T > C SNP in the HSPB1 gene produced a significant difference in meat quantity, and the performance of animals with the CC homozygous genotype was higher than that of other genotypes (Suh et al, 2020). In a study into the production of high-quality beef, the CT heterozygote genotype of c.-64T > C SNP in the ADH1C gene was found to have an increased marbling score compared with the other genotypes (Peng et al, 2017).…”

“…SNPs can be used as DNA markers for producing high-quality Korean beef as discriminating meat-related genetic factors (Kim et al, 2009). Using the c.-64T > C SNP of the ADH1C gene, Ward et al (2012) reported that marbling in cattle with the TT genotype of the ADH1C gene was higher than that in TC and CC genotypes during 5 months of restriction of vitamin A in Angus crossbred steers (Ward et al, 2012). Peng et al (2017) analyzed the association of the c.-64T > C SNP of the ADH1C gene with intramuscular fat deposition in Korean cattle during experiments with vitamin-A restriction in Korean cattle steers (Peng et al, 2017).…”