Proteomic identification of an embryo-specific 1Cys-Prx promoter and analysis of its activity in transgenic rice

Kim, Je Hein; Jung, In‐Ha; Kim, Dool Yi; Fanata, Wahyu Indra Duwi; Son, Bo Hwa; Yoo, Ji Myong; Harmoko, Rikno; Ko, Ki Seong; Moon, Jeong Chan; Jang, Ho Hee; Kim, Woe Yeon; Kim, Jae‐Yean; Lim, Chae Oh; Lee, Sang Yeol; Lee, Kyun Oh

doi:10.1016/j.bbrc.2011.03.120

Cited by 11 publications

(7 citation statements)

References 20 publications

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“…The seedlings were grown for 2 weeks in a growth chamber with supplemental lighting (8 h dark/16 h light; 200 lmol m À2 sec À1 ) and a day/night temperature regime of 30/25°C, and then were transferred to a greenhouse (sunlight). Embryo-derived rice callus was induced and maintained as described previously (Kim et al, 2011). Arabidopsis thaliana Columbia (Col-0), cgl1-T, and complemented cgl1-T seeds were sterilized and grown for 5 days on MS medium containing salt mixture (Duchefa), pH 5.8 supplemented with 3% sucrose and 0.25% gellan gum in a growth chamber with supplemental lighting (8 h dark/16 h light; 120 lmol m À2 sec À1 ) at 22°C and 70% humidity.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

N‐glycan maturation is crucial for cytokinin‐mediated development and cellulose synthesis in Oryza sativa

et al. 2013

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SUMMARYTo explore the physiological significance of N-glycan maturation in the plant Golgi apparatus, gnt1, a mutant with loss of N-acetylglucosaminyltransferase I (GnTI) function, was isolated in Oryza sativa. gnt1 exhibited complete inhibition of N-glycan maturation and accumulated high-mannose N-glycans. Phenotypic analyses revealed that gnt1 shows defective post-seedling development and incomplete cell wall biosynthesis, leading to symptoms such as failure in tiller formation, brittle leaves, reduced cell wall thickness, and decreased cellulose content. The developmental defects of gnt1 ultimately resulted in early lethality without transition to the reproductive stage. However, callus induced from gnt1 seeds could be maintained for periods, although it exhibited a low proliferation rate, small size, and hypersensitivity to salt stress. Shoot regeneration and dark-induced leaf senescence assays indicated that the loss of GnTI function results in reduced sensitivity to cytokinin in rice. Reduced expression of A-type O. sativa response regulators that are rapidly induced by cytokinins in gnt1 confirmed that cytokinin signaling is impaired in the mutant. These results strongly support the proposed involvement of N-glycan maturation in transport as well as in the function of membrane proteins that are synthesized via the endomembrane system.

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Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

N‐glycan maturation is crucial for cytokinin‐mediated development and cellulose synthesis in Oryza sativa

et al. 2013

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“…They include 1CYS‐PEROXIREDOXIN ( 1Cys‐Prx /Os07g44430 ), which shows the strongest expression in the presence of abscisic acid (ABA) and is characteristic of pharmaceutical production in plants. Driven by the 1Cys‐Prx promoter, GUS expression is strong in the embryo and aleurone layer in transgenic rice seeds (Kim et al ). The promoter contains putative seed‐specific cis ‐acting elements, RYE and the abscisic acid‐responsive element (ABRE), which can help the promoter to drive embryo‐specific expression.…”

Section: Tissue‐specific Promotersmentioning

confidence: 99%

“…In vegetative tissues, ABA levels increase significantly in response to different environmental cues, including drought, salinity, or chilling (Ye et al ). Transcripts for 1CYS‐PEROXIREDOXIN ( 1Cys‐Prx / Os07g44430 ) are most abundant in the presence of ABA, and GUS expression driven by the 1Cys‐Prx promoter is increased by ABA treatment (Kim et al ). SIMILAR TO EMBRYONIC ABUNDANT PROTEIN (O SEM/Os05g28210 ), a rice gene homologous to Em from wheat, encodes a late‐embryogenesis abundant protein.…”

Section: Condition‐dependent Promotersmentioning

confidence: 99%

Rice tissue‐specific promoters and condition‐dependent promoters for effective translational application

Jeong

Jung

2015

JIPB

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Rice (Oryza sativa) is one of the most important staple food crops for more than half of the world's population. The demand is increasing for food security because of population growth and environmental challenges triggered by climate changes. This scenario has led to more interest in developing crops with greater productivity and sustainability. The process of genetic transformation, a major tool for crop improvement, utilizes promoters as one of its key elements. Those promoters are generally divided into three types: constitutive, spatiotemporal, and condition-dependent. Transcriptional control of a constitutive promoter often leads to reduced plant growth, due to a negative effect of accumulated molecules during cellular functions or energy consumption. To maximize the effect of a transgene on transgenic plants, it is better to use condition-dependent or tissuespecific promoters. However, until now, those types have not been as widely applied in crop biotechnology. In this review, we introduce and discuss four groups of tissue-specific promoters (50 promoters in total) and six groups of condition-dependent promoters (27 promoters). These promoters can be utilized to fine-tune desirable agronomic traits and develop crops with tolerance to various stresses, enhanced nutritional value, and advanced productivity.

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“…Embryo in plant functions as an important nutrient source for later stages of seedling growth because plants accumulate storage proteins, carbohydrates, and oils in their embryonic cells. Although few embryo-specific promoters have been reported, tissue-specific promoters that drive transgene expression in embryos have been a focus of attention for their special use in studying embryo growth and development, in addition to accumulate seed oil or recombinant protein in monocotyledon (José-Estanyol et al 2005;Kim et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Isolation of a maize ZmCI-1B promoter and characterization of its activity in transgenic maize and tobacco

Liu

et al. 2015

Plant Cell Rep

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The 2-kb ZmCI - 1B promoter is active in the root and embryo and induced by wounding in maize and the 220-bp 5'-deleted segment maybe the minimal promoter. The subtilisin-chymotrypsin inhibitor gene, CI-1B of Zea mays (ZmCI-1B), has been suggested to induce the maize defense system to resist insect attack. Real-time RT-PCR showed that ZmCI-1B gene exhibited especially high expression in roots and embryos. The 2-kb full-length promoter of ZmCI-1B gene was isolated from the maize genome and used to drive expression of a beta-glucuronidase (GUS) reporter gene for transient expression and stable expression analysis in maize. The results of GUS histochemical staining in transgenic maize plants revealed that the ZmCI-1B promoter induced GUS expression preferentially in roots and embryos and in response to wounding. A series of 5'-deleted segments of the ZmCI-1B promoter were cloned individually to drive GUS expression for further analysis. Deletion analysis combined with the histochemical staining of transgenic tobacco plants revealed 220-bp segment could drive GUS in a tissue-specific and wounding-induced expression in tobacco; thus, it maybe the minimally active promoter of ZmCI-1B gene. Furthermore, it revealed that the ZmCI-1B promoter contained tissue-specific and wounding-induced elements.

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Proteomic identification of an embryo-specific 1Cys-Prx promoter and analysis of its activity in transgenic rice

Cited by 11 publications

References 20 publications

N‐glycan maturation is crucial for cytokinin‐mediated development and cellulose synthesis in Oryza sativa

N‐glycan maturation is crucial for cytokinin‐mediated development and cellulose synthesis in Oryza sativa

Rice tissue‐specific promoters and condition‐dependent promoters for effective translational application

Isolation of a maize ZmCI-1B promoter and characterization of its activity in transgenic maize and tobacco

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