Proteomic Profiling of the Liver, Hepatic Lymph Nodes, and Spleen of Buffaloes Infected with Fasciola gigantica

Abstract

In the present study, we used an isobaric tag for relative and absolute quantitation (iTRAQ) proteomics technology to characterize the differentially expressed proteins (DEPs) in the liver, hepatic lymph nodes (hLNs), and spleen of buffaloes infected with Fasciola gigantica (F. gigantica). We also used the parallel reaction monitoring (PRM) method to verify the expression levels of the DEPs in the three infected tissues. At three days post-infection (dpi), 225, 1821, and 364 DEPs were detected in the liver, hLNs, and spleen, respectively. At 42 dpi, 384, 252, and 214 DEPs were detected in the liver, hLNs, and spleen, respectively. At 70 dpi, 125, 829, and 247 DEPs were detected in the liver, hLNs, and spleen, respectively. Downregulation of metabolism was prominent in infected livers at all time points, and upregulation of immune responses was marked in the hLNs during early infection (three dpi); however, no changes in the immune response were detected at the late stages of infection (42 and 70 dpi). Compared to the hLNs, there was no significant upregulation in the levels of immune responses in the infected spleen. All the identified DEPs were used to predict the subcellular localization of the proteins, which were related to extracellular space and membrane and were involved in host immune responses. Further PRM analysis confirmed the expression of 18 proteins. These data provide the first simultaneous proteomic profiles of multiple organs of buffaloes experimentally infected with F. gigantica.

Genome-Wide Association Study Using Whole-Genome Sequence Data for Fertility, Health Indicator, and Endoparasite Infection Traits in German Black Pied Cattle

“…For RES-FH, WGS data revealed a large number of candidate genes and related pathways previously described for F. hepatica infections via transcriptomic studies [51,53,56,59,63], indicating the advantage of WGS data to identify causal mutations for complex health traits in dairy cattle. We identified the fibroblast growth factor receptor 1 (FGFR1) gene on BTA 27 associated with RES-FH.…”

“…RES-FH PLCB1 (13), PTK2 (14) Pathway was associated with Fasciola gigantica infections in Buffalo via proteomics analysis [53]; pathway associated with F. hepatica infections in mice [54].…”

Transcriptomic landscape of hepatic lymph nodes, peripheral blood lymphocytes and spleen of swamp buffaloes infected with the tropical liver fluke Fasciola gigantica

“…At 3, 42, and 70 dpi, the presence of F . gigantica in infected liver and bile duct was confirmed by examination, as described previously [ 8 , 9 ] and buffalo’s hLNs, pBLs and spleen tissues were collected. Each tissue was washed with PBS several times and put into sterile tubes and stored at -80°C, until use.…”

“…Despite significant efforts [ 7 , 9 , 12 , 13 ], knowledge about the immune responses in buffaloes experimentally infected with F . gigantica remains limited.…”

“…Previously, we have identified many differentially expressed proteins (DEPs) in F . gigantica -infected buffalo tissues, including hLNs, serum, and spleen, using iTRAQ proteomics [ 7 , 9 ]. However, the molecular mechanisms underlying the pathological effects of F .…”