Proteomics: a promising tool for research on sex-related differences in dioecious plants

Yang, Le; Gong, Fangping; Xiong, Erhui; Wang, Wei

doi:10.3389/fpls.2015.00954

Cited by 5 publications

(6 citation statements)

References 27 publications

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“…However, proteomics study generally depends on the availability of genomic/transcriptomic data, which is limited for most of the dioecious species. Hence, there are only few studies, which have employed proteomics approach in order to understand sex determination and differentiation in dioecious plants [38]. One such study carried out in Asparagus identified differentially accumulated proteins in the form of spots on 2-D gels from flowers of male and female plants [39].…”

Section: Introductionmentioning

confidence: 99%

“…Proteomic approaches has also been attempted to identify sex-linked proteins in Ginkgo biloba . A 28 kDa protein specific to male and 36 kDa as well as 92 kDa proteins specific to female inflorescences have been identified [38]. Recently, Manzano , et al [41] showed that overexpression of aerolysin-like protein from the dioecious plant R. acetosa induces male sterility in transgenic tobacco.…”

Section: Introductionmentioning

confidence: 99%

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Flower bud proteome reveals modulation of sex-biased proteins potentially associated with sex expression and modification in dioecious Coccinia grandis

et al. 2019

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Background Dioecy is an important sexual system wherein, male and female flowers are borne on separate unisexual plants. Knowledge of sex-related differences can enhance our understanding in molecular and developmental processes leading to unisexual flower development. Coccinia grandis is a dioecious species belonging to Cucurbitaceae, a family well-known for diverse sexual forms. Male and female plants have 22A + XY and 22A + XX chromosomes, respectively. Previously, we have reported a gynomonoecious form (22A + XX) of C. grandis bearing morphologically hermaphrodite flowers (GyM-H) and female flowers (GyM-F). Also, we have showed that foliar spray of AgNO 3 on female plant induces morphologically hermaphrodite bud development (Ag-H) despite the absence of Y-chromosome. Results To identify sex-related differences, total proteomes from male, female, GyM-H and Ag-H flower buds at early and middle stages of development were analysed by label-free proteomics. Protein search against the cucumber protein sequences (Phytozome) as well as in silico translated C. grandis flower bud transcriptome database, resulted in the identification of 2426 and 3385 proteins (FDR ≤ 1%), respectively. The latter database was chosen for further analysis as it led to the detection of higher number of proteins. Identified proteins were annotated using BLAST2GO pipeline. SWATH-MS-based comparative abundance analysis between Female_Early_vs_Male_Early, Ag_Early_vs_Female_Early, GyM-H_Middle_vs_Male_Middle and Ag_Middle_vs_ Male_Middle led to the identification of 650, 1108, 905 and 805 differentially expressed proteins, respectively, at fold change ≥1.5 and P ≤ 0.05. Ethylene biosynthesis-related candidates as highlighted in protein interaction network were upregulated in female buds compared to male buds. AgNO 3 treatment on female plant induced proteins related to pollen development in Ag-H buds. Additionally, a few proteins governing pollen germination and tube growth were highly enriched in male buds compared to Ag-H and GyM-H buds. Conclusion Overall, current proteomic analysis provides insights in the identification of key proteins governing dioecy and unisexual flower development in cucurbitaceae, the second largest horticultural family in terms of economic importance. Also, our results suggest that the ethylene-mediated stamen inhibition might be conserved in dioecious C. grandis similar to its monoecious cucurbit relatives. Further, male-biased proteins associated with pollen germination and tube growth identified here can help in understanding pollen fertility. Electronic supplementary material The online version of this article (10.1186/s12870-019-1937-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Flower bud proteome reveals modulation of sex-biased proteins potentially associated with sex expression and modification in dioecious Coccinia grandis

et al. 2019

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“…In the above formula, (T ′ /T) represents the weights, k represents the regulator between the bilayers, and value range of [1].…”

Section: Design Of Double-dimensional Ecological Cultivationmentioning

confidence: 99%

“…e increasing contradiction between man and land, the decrease of arable land, pollution, destruction of land resources, the serious shortage of water resources, the low efficiency of water production, and the low utilization rate of fertilizer have become the important factors restricting the sustainable development of agriculture in China. As a result, the planting area of fruit trees is decreasing, and facility cultivation has become a key method for growing fruit trees in recent years [1]. It is for this reason that facility cultivation is one of the important signs of agricultural modernization in seasons or areas that are not conducive to growing fruit trees and other crops [2,3].…”

Section: Introductionmentioning

confidence: 99%

Study on Double-Layer Stereo Ecological Cultivation Technology of Greenhouse Gardening Fruit Trees

Chen

Boddu

2022

Journal of Food Quality

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Global food demand can be met by agricultural technology. But the increase in demand also needs effective planting and sufficient illumination in order for crops to grow. In order to solve the problems of low planting efficiency and insufficient illumination in traditional greenhouse horticultural fruit tree planting, we propose a three-dimensional double-layer ecological cultivation technology for greenhouse horticultural fruit trees. The design is a double-layered three-dimensional cultivation model. After the growth rate of horticultural fruit trees is determined, the optimal distance between the two-dimensional cultivation layers is determined by weighted Euclidean distance, and the double-layer three-dimensional ecological cultivation mode of greenhouse horticultural fruit trees is designed. Through the analysis of the irradiance of the double bed planting mode, the design of the light environment in horticultural fruit tree planting is completed. A principal component analysis was used to determine the amount of soil components in double-layer three-dimensional ecological cultivation of greenhouse horticultural fruit trees, and the technology of double-layer three-dimensional ecological cultivation was developed. The results show that the planting efficiency of horticultural fruit trees planted by the technology is always higher than 90%, and the illumination in the planting environment is sufficient.

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“…Since sex differentiation has recently become a research hotspot, proteomics has been demonstrated as a powerful tool to examine sex-related differences in plants and even the stress adaption changes from dioecious plants [15]. For example, comparative proteomic analysis of the mitochondrial proteome between sterile rice and fertile rice revealed a clade of regulatory proteins with decreased abundance in mitochondrial complex leading to cytoplasmic male sterility in sterile rice [16].…”

Section: Introductionmentioning

confidence: 99%

SWATH-MS based quantitive proteomics reveal regulatory metabolism and networks of androdioecy breeding system in Osmanthus fragrans

Duan

Zhang

et al. 2021

BMC Plant Biol

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Background The fragrant flower plant Osmanthus fragrans has an extremely rare androdioecious breeding system displaying the occurrence of males and hermaphrodites in a single population, which occupies a crucial intermediate stage in the evolutionary transition between hermaphroditism and dioecy. However, the molecular mechanism of androdioecy plant is very limited and still largely unknown. Results Here, we used SWATH-MS-based quantitative approach to study the proteome changes between male and hermaphroditic O. fragrans pistils. A total of 428 proteins of diverse functions were determined to show significant abundance changes including 210 up-regulated and 218 down-regulated proteins in male compared to hermaphroditic pistils. Functional categorization revealed that the differentially expressed proteins (DEPs) primarily distributed in the carbohydrate metabolism, secondary metabolism as well as signaling cascades. Further experimental analysis showed the substantial carbohydrates accumulation associated with promoted net photosynthetic rate and water use efficiency were observed in purplish red pedicel of hermaphroditic flower compared with green pedicel of male flower, implicating glucose metabolism serves as nutritional modulator for the differentiation of male and hermaphroditic flower. Meanwhile, the entire upregulation of secondary metabolism including flavonoids, isoprenoids and lignins seem to protect and maintain the male function in male flowers, well explaining important feature of androdioecy that aborted pistil of a male flower still has a male function. Furthermore, nine selected DEPs were validated via gene expression analysis, suggesting an extra layer of post-transcriptional regulation occurs during O. fragrans floral development. Conclusion Taken together, our findings represent the first SWATH-MS-based proteomic report in androdioecy plant O. fragrans, which reveal carbohydrate metabolism, secondary metabolism and post-transcriptional regulation contributing to the androdioecy breeding system and ultimately extend our understanding on genetic basis as well as the industrialization development of O. fragrans.

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Proteomics: a promising tool for research on sex-related differences in dioecious plants

Cited by 5 publications

References 27 publications

Flower bud proteome reveals modulation of sex-biased proteins potentially associated with sex expression and modification in dioecious Coccinia grandis

Flower bud proteome reveals modulation of sex-biased proteins potentially associated with sex expression and modification in dioecious Coccinia grandis

Study on Double-Layer Stereo Ecological Cultivation Technology of Greenhouse Gardening Fruit Trees

SWATH-MS based quantitive proteomics reveal regulatory metabolism and networks of androdioecy breeding system in Osmanthus fragrans

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