Proteomics: methodologies and applications to the study of human diseases

Barbosa, Eduardo Buzolin; Vidotto, Alessandra; Polachini, Giovana Mussi; Henrique, Tiago; Marqui, Alessandra Bernadete Trovó de; Tajara, Eloíza H.

doi:10.1016/s2255-4823(12)70209-6

Cited by 14 publications

(10 citation statements)

References 138 publications

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“…In a previous study, our group found a paracentric inversion in the short arm of chromosome 3 (3p25-26) in MSC isolated from one umbilical cord. In this context, studying the MSC-UCh proteins is relevant since these molecules are directly or indirectly responsible for controlling all or almost all biological processes (Barbosa et al, 2012). Proteomics studies the set of proteins in a descriptive and quantitative way, as well as how the protein levels vary in the population depending on the environment or interactions with other proteins (Valledor & Jorrín, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Proteomics studies the set of proteins in a descriptive and quantitative way, as well as how the protein levels vary in the population depending on the environment or interactions with other proteins (Valledor & Jorrín, 2011). The proteome is dynamic and changes according to the physiological status and cell differentiation phases (Barbosa et al, 2012). In a quantitative proteomic analysis, 463 surface proteins were found in MSC submitted to differentiation in osteoblasts (Foster et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Study of comparative proteome between normal and inverted karyotypes of human mesenchymal stem cells

Santos

Câmara

Meira

2020

Acta Sci. Biol. Sci.

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Multipotent mesenchymal stem cells have been expanded in vitro for cellular therapy in numerous clinical settings without standardized culture conditions or quality-control schemes. The in vitro expansion is necessary to obtain sufficient cells for clinical applications. However, the expansion may induce genetic and functional abnormalities which may affect the safety and functionality of MSC, especially the chromosomal stability. This study aimed to investigate the protein profile of umbilical cord-derived MSC with normal and inverted karyotypes after expansion in the laboratory. Mass spectrometry analysis was performed and the Bradford method, Scaffold software, String and Cytoscape databases were employed to measure and characterize the protein content of umbilical cord-derived MSC. Networks of protein interactions, hub and bottleneck proteins were identified by proteomics and systems biology approaches. We found that proteins related to cellular stress were super expressed in inverted karyotype cells. Moreover, a high expression of Serpine 1, RHOA, and CTSB was found in these cells, which are proteins related to cancer. The albumin and ubiquitin proteins have been associated with a positive prognosis in cancer and cellular stress, and were up- and down-regulated in normal karyotype cells, respectively. The results suggests that the paracentric inversion inv(3)(p25p13) induced some type of cellular stress and genetic instability in human mesenchymal stem cells. These analyses showed the importance of carrying out studies related to the genetic instability of human mesenchymal stem cells using the protein expression profile as a parameter.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Study of comparative proteome between normal and inverted karyotypes of human mesenchymal stem cells

Santos

Câmara

Meira

2020

Acta Sci. Biol. Sci.

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“…The serum based OSCC biomarkers may reveal the disease initiation and progression . The complex cellular and serum proteomes can be resolved by various techniques, based on gel matrix based and gel matrix free proteomic strategies, comprising one dimensional gel electrophoresis, two dimensional gel electrophoresis (2DE), two dimensional fluorescence differential gel electrophoresis, nano‐LC MS, and others . In comparison to latter, the gel matrix based proteomic strategies separate proteome to a larger extent yet in cost effective manner .…”

Section: Introductionmentioning

confidence: 99%

Assessment of cellular and serum proteome from tongue squamous cell carcinoma patient lacking addictive proclivities for tobacco, betel nut, and alcohol: Case study

Khowal

Naqvi

Monga

et al. 2018

J of Cellular Biochemistry

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The intriguing molecular pathways involved in oral carcinogenesis are still ambiguous. The oral squamous cell carcinoma (OSCC) ranks as the most common type constituting more than 90% of the globally diagnosed oral cancers cases. The elevation in the OSCC incidence rate during past 10 years has an alarming impression on human healthcare. The major challenges associated with OSCC include delayed diagnosis, high metastatic rates, and low 5-year survival rates. The present work foundations on reverse genetic strategy and involves the identification of genes showing expressional variability in an OSCC case lacking addictive proclivities for tobacco, betel nut, and/or alcohol, major etiologies. The expression modulations in the identified genes were analyzed in 16 patients comprising oral pre-cancer and cancer histo-pathologies. The genes SCCA1 and KRT1 were found to down regulate while DNAJC13, GIPC2, MRPL17, IG-Vreg, SSFA2, and UPF0415 upregulated in the oral pre-cancer and cancer pathologies, implicating the genes as crucial players in oral carcinogenesis.

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“…Clinical TCM syndrome studies using omics technologies only were the beginning [11]. However, proteomics complements genomics technology and metabolomics technology, and has been successfully used to study CM syndromes, such as syndromes in chronic myocardial ischemia [12], coronary heart disease [13], chronic hepatitis B [14], tuberculosis [15], et al The results of proteomic approaches are useful in clinical research areas, such as diagnosis, disease subtype classification, characterization of metabolic pathways, biomarker quantification, and therapeutic target generation [16]. However, most of the above TCM syndrome studies used classical proteomics approaches (two-dimensional gel electrophoresis, 2-DE).…”

Section: Introductionmentioning

confidence: 99%

Serum proteomic-based analysis by iTRAQ of damp-heat impeding syndrome of rheumatoid arthritis

Sun

Zhou

et al. 2015

European Journal of Integrative Medicine

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Proteomics: methodologies and applications to the study of human diseases

Cited by 14 publications

References 138 publications

Study of comparative proteome between normal and inverted karyotypes of human mesenchymal stem cells

Study of comparative proteome between normal and inverted karyotypes of human mesenchymal stem cells

Assessment of cellular and serum proteome from tongue squamous cell carcinoma patient lacking addictive proclivities for tobacco, betel nut, and alcohol: Case study

Serum proteomic-based analysis by iTRAQ of damp-heat impeding syndrome of rheumatoid arthritis

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