Proteomics of aluminum tolerance in plants

Zheng, Lu; Lan, Ping; Shen, Ren Fang; Li, Wen Feng

doi:10.1002/pmic.201300252

Cited by 31 publications

(36 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Aluminum, which is also not contained in the CA medium, has been particularly investigated for the inhibition of plant growth. 16,17) Although the fact that these elements containing aluminum and other metal elements were involved comprehensively in the culture medium, they did not directly affect algal growth. Consequently, they showed no marked toxicity to algae in slag eluate.…”

Section: Evaluation Of Effects Of Slag Eluate On the Algalmentioning

confidence: 99%

Bioassay of Components Eluted from Electric Arc Furnace Steel Slag Using Microalgae <i>Chlorella</i>

Takahashi

Yokoyama

2016

ISIJ International

View full text Add to dashboard Cite

Several amounts of electric arc furnace (EAF) steel slag ultimately end up in final landfill sites. After developing a method to estimate the impacts of eluate from slag, particularly on phytoplankton, this study assessed novel slag applications to aquatic environments. First, metal components were eluted from EAF slags of common steel or stainless steel with a leaching condition based on JIS K 0058-1. The metal components in slag were analyzed using emission spectrochemical analyses. After incubation of green algae Chlorella as phytoplankton with culture media containing eluates from the respective slags, the effects of the respective eluates on algae were investigated using microscopy and flow cytometry. Results demonstrated that concentrations of metal effluents from slags, even for EAF steel slag, which included more hazardous materials than common slag, were almost all lower than environmental quality standards for effluent and drinking water. Analyses of algal cells treated with each eluate revealed that eluate induced neither lethality nor growth inhibition. Instead of cytotoxicity, the addition of each eluate enhanced algal growth. Infrared spectroscopy and potentiometry using a diaphragm-type electrode to measure aquatic CO 2 revealed that metal components from both slags in media produced greater amounts of aquatic CO 2 available for photosynthesis, thereby enhancing algal proliferation. Taken together, results show that using EAF slag in aquatic environments might be beneficial, not toxic, for photosynthetic organisms. Furthermore, bioassay using flow cytometry can estimate vigorous and aberrant algal growth simultaneously.

show abstract

Section: Evaluation Of Effects Of Slag Eluate On the Algalmentioning

confidence: 99%

Bioassay of Components Eluted from Electric Arc Furnace Steel Slag Using Microalgae <i>Chlorella</i>

Takahashi

Yokoyama

2016

ISIJ International

View full text Add to dashboard Cite

show abstract

“…[1,2,7,8] Despite that, the response mechanisms triggered by Al are still poorly understood; however, more recently this subject has been investigated at the molecular level. [9][10][11][12][13] Nevertheless, some plants are extremely tolerant to Al. This is the case of Qualea grandiflora Mart., a compulsory Al accumulating species of Brazilian savanna.…”

Section: Doi: 101002/pmic201900148mentioning

confidence: 99%

Proteome Dataset of Qualea grandiflora Mart. (Vochysiaceae) by LC–MS/MS Label‐Free Identification in Response to Aluminum

et al. 2019

View full text Add to dashboard Cite

This dataset brief is about the descriptive proteome of Qualea grandiflora plants by label free mass spectrometry (LC–MS/MS). Q. grandiflora is a plant that accumulates aluminum (Al) in high quantities and requires it for growth and development. Although quite relevant for the understanding of Al effects on plants, the proteome of Q. grandiflora has not been studied yet. Therefore, the current proteome analysis identifies a total of 2010 proteins. Furthermore, the identified Q. grandiflora root proteins are associated with several crucial molecular functions, biological processes, and cellular sites. Hence, the proteome analysis of Q. grandiflora will contribute to unravel how plants evolved to cope with high levels of Al in soils. All data can be accessed at the Centre for Computational Mass Spectrometry – MassIVE MSV000082284 – https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=adb9647282a5421a9cffe3124c060f46

show abstract

“…As this method can analyze and quantify up to eight phenotypes with high resolution (Ross et al, 2004; Pierce et al, 2008), it has been widely used in model plants such as Arabidopsis (Lan et al, 2011) and rice (Wang et al, 2014), but also has provided a platform to profile and understand the non-model species through comparative proteomics (Yang Y. et al, 2011; Xiong et al, 2015). As a whole system approach, this proteomics technique provides a complementary strategy to unravel the mechanisms underlying plant Al tolerance (Jedmowski et al, 2014; Zheng et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance

et al. 2017

View full text Add to dashboard Cite

Aluminum (Al) toxicity inhibits root growth and limits crop yields on acid soils worldwide. However, quantitative information is scarce on protein expression profiles under Al stress in crops. In this study, we report on the identification of potential Al responsive proteins from root tips of Al sensitive BR007 and Al tolerant SC566 sorghum lines using a strategy employing iTRAQ and 2D-liquid chromatography (LC) coupled to MS/MS (2D-LC-MS/MS). A total of 771 and 329 unique proteins with abundance changes of >1.5 or <0.67-fold were identified in BR007 and SC566, respectively. Protein interaction and pathway analyses indicated that proteins involved in the antioxidant system were more abundant in the tolerant line than in the sensitive one after Al treatment, while opposite trends were observed for proteins involved in lignin biosynthesis. Higher levels of ROS accumulation in root tips of the sensitive line due to decreased activity of antioxidant enzymes could lead to higher lignin production and hyper-accumulation of toxic Al in cell walls. These results indicated that activities of peroxidases and the balance between production and consumption of ROS could be important for Al tolerance and lignin biosynthesis in sorghum.

show abstract

Proteomics of aluminum tolerance in plants

Cited by 31 publications

References 74 publications

Bioassay of Components Eluted from Electric Arc Furnace Steel Slag Using Microalgae <i>Chlorella</i>

Bioassay of Components Eluted from Electric Arc Furnace Steel Slag Using Microalgae <i>Chlorella</i>

Proteome Dataset of Qualea grandiflora Mart. (Vochysiaceae) by LC–MS/MS Label‐Free Identification in Response to Aluminum

Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance

Contact Info

Product

Resources

About