Protoplasmic streaming, cytochalasin B, and growth of the pollen tube

Mascarenhas, Joseph P.; LaFountain, James R.

doi:10.1016/s0040-8166(72)80002-8

Cited by 105 publications

(52 citation statements)

References 8 publications

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“…An unperturbed F-actin cytoskeleton is required for the establishment of polarity in Fucus and Pelvetia embryos (Quatrano, 1973;Alessa and Kropf, 1999). Actin filaments also are the primary cytoskeletal determinant of polarized tip growth in pollen tubes (Mascarenhas and LaFountain, 1972;Heslop-Harrison et al, 1986;Gibbon et al, 1999;Fu et al, 2001). Much of leaf and cotton trichome growth is caused by polarized diffuse growth, and the resulting pharmacological sensitivities are quite different from those of pollen tubes.…”

Section: Introductionmentioning

confidence: 99%

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The Arabidopsis SPIKE1 Gene Is Required for Normal Cell Shape Control and Tissue Development

et al. 2002

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Regulated growth and cell shape control are fundamentally important to the function of plant cells, tissues, and organs. The signal transduction cascades that control localized growth and cell shape, however, are not known. To better understand the relationship between cytoskeletal organization, organelle positioning, and regulated vesicle transport, we conducted a forward genetic screen to identify genes that regulate cytoskeletal organization in plants. Because of the distinct requirements for microtubules and actin filaments during leaf trichome development, a trichome-based morphology screen is an efficient approach to identify genes that affect cytoplasmic organization. The seedling lethal spike1 mutant was identified based on trichome, cotyledon, and leaf-shape defects. The predicted SPIKE1 protein shares amino acid identity with a large family of adapter proteins present in humans, flies, and worms that integrate extracellular signals with cytoskeletal reorganization. Both the trichome phenotype and immunolocalization data suggest that SPIKE1 also is involved in cytoskeletal reorganization. The assembly of laterally clustered foci of microtubules and polarized growth are early events in cotyledon development, and both processes are misregulated in spike1 epidermal cells. INTRODUCTIONIn multicellular organisms, specialized cytoplasmic organization and cell shape underlie the unique functions of cells, tissues, and organs. It is reasonable to propose that the dynamic properties of the microtubule and actin cytoskeletons and the proteins that bind them underlie much of the observed asymmetry in plant cells. During leaf and root trichoblast morphogenesis, organized actin filaments and microtubules are required (Bibikova et al., 1999;Mathur et al., 1999;Szymanski et al., 1999;Baluska et al., 2000). We are using the diagnostic shape defects of cytoskeleton-disrupted leaf trichomes to guide mutant screens for essential genes that participate in cytoskeletal reorganization and morphogenesis.Decades of cytological and biochemical research have demonstrated the importance of the actin filament and microtubule cytoskeletons during plant morphogenesis (for reviews of the interphase microtubule and actin cytoskeletons in plant cells, see Giddings and Staehelin, 1991;Cyr, 1994;Staiger, 2000). In most plant cell types, the interphase microtubule array is cortical. The most commonly cited function of the cortical microtubule array is the regulation of the alignment of newly synthesized cellulose microfibrils. However, there are cases in which microtubules and cellulose microfibrils are not coaligned (Baskin et al., 1999; Wasteneys, 2000). The interphase actin filament cytoskeleton is composed of bundled transvacuolar filaments, nucleus-associated filaments, and cortical actin filaments (Traas et al., 1987). Actin filaments provide both a scaffolding for the relatively immobile network of the endoplasmic reticulum and tracks for the rapid intracellular transport of Golgi stacks (Satiat-Jeunemaitre and Hawes, 1996;Boevink...

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Section: Introductionmentioning

confidence: 99%

“…The Plant Cell of polarized tip growth in pollen tubes (Mascarenhas and LaFountain, 1972;Heslop-Harrison et al, 1986;Gibbon et al, 1999;Fu et al, 2001). Much of leaf and cotton trichome growth is caused by polarized diffuse growth, and the resulting pharmacological sensitivities are quite different from those of pollen tubes.…”

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confidence: 99%

The Arabidopsis SPIKE1 Gene Is Required for Normal Cell Shape Control and Tissue Development

et al. 2002

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“…Recent work has indicated that periodic elongation of short actin bundles into the apical dome occurs between the growth pulses (Fu et al, 2001). Growth is supported by exocytosis of synthetic materials that are delivered by the highly active vesicle-trafficking system, visualized as cytoplasmic streaming, that transports secretory vesicles along the actin cytoskeleton toward the apical region (Mascarenhas and LaFountain, 1972;Derksen et al, 1995;Miller et al, 1995;Cai et al, 1997;Yokuta et al, 1999). The vesicles accumulate at the region where most actin bundles terminate, near the distal boundary of the apical dome.…”

Section: Introductionmentioning

confidence: 99%

Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by Inositol 3,4,5,6-Tetrakisphosphate

et al. 2002

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“…9 In angiosperms, pollen tubes show a strong cytoplasmic streaming of organelles, which move along actin filaments, as shown by earlier studies with cytoskeleton inhibitors. 59,60 Although less well supported, it is evident that microtubules also participate in regulation of …”

Section: Higher Plant Cellsmentioning

confidence: 99%

Structural and biomechanical basis of mitochondrial movement in eukaryotic cells

Wan¹,

Kalyanasundaram²,

Zhu³

2013

IJN

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Protoplasmic streaming, cytochalasin B, and growth of the pollen tube

Cited by 105 publications

References 8 publications

The Arabidopsis SPIKE1 Gene Is Required for Normal Cell Shape Control and Tissue Development

The Arabidopsis SPIKE1 Gene Is Required for Normal Cell Shape Control and Tissue Development

Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by Inositol 3,4,5,6-Tetrakisphosphate

Structural and biomechanical basis of mitochondrial movement in eukaryotic cells

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