Prussian Blue and enzyme bulk-modified screen-printed electrodes for hydrogen peroxide and glucose determination with improved storage and operational stability

Ricci, Francesco; Amine, Aziz; Tuță, Cătălin Stelian; Ciucu, Anton Alexandru; Lucarelli, Fausto; Palleschi, Giuseppe; Moscone, Danila

doi:10.1016/s0003-2670(03)00403-3

Cited by 125 publications

(66 citation statements)

References 25 publications

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“…This result was superior to those of the previous studies. 36,52,53 Amperometric response and calibration curve Figure 6a shows a typical current-time plot for the sensor at 0.1 V on successive addition of 300 mM glucose in 0.06 M pH 7.0 PBS. When an aliquot of glucose is added into the buffer solution, the reduction current rises steeply to reach a stable value as a result of the reduction of H2O2 produced from enzymatic reaction, while direct catalysis of glucose is not observed at blank titania sol-gel film electrode in the studied potential window (Fig.…”

Section: Condition Optimization For Glucose Sensingmentioning

confidence: 99%

“…[35][36][37][38] Since this catalytic reaction occurs in a relative low potential region, interferences from the coexisting substances such as AA and UA are negligible. A large number of oxidase enzyme-based biosensors for clinical, environmental and food analysis can be obtained by initially electrodepositing PB, followed by immobilizing oxidase enzyme.…”

Section: Introductionmentioning

confidence: 99%

“…38 Ricci et al have reported an improvement in PB stability, even in alkaline media, when PB was chemically deposited on the surface of a screen-printed carbon electrode (SPCE) in the presence of KCl. 36,46 Instead of SPCE, screen-printed Au and Pt electrodes modified with electrochemically deposited PB have 1426 ANALYTICAL SCIENCES NOVEMBER 2008, VOL. 24 been developed and used as the basis for construction of amperometric glucose biosensors through further modification with enzymes immobilized in a Nafion membrane.…”

Section: Introductionmentioning

confidence: 99%

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An Amperometric Glucose Biosensor Based on Titania Sol-gel/Prussian Blue Composite Film

2008

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An improved amperometric glucose biosensor was constructed by immobilizing glucose oxidase (GOD) in a titania sol-gel film, which was prepared by a vapor deposition method, on a Prussian Blue (PB)-modified electrode. The method combined the merits of immobilizing biomolecules in the titania sol-gel film by vapor deposition method and the synergic catalysis effects of PB and GOD molecules. Results showed that the fabricated titania sol-gel/PB membrane possessed high surface area, good mechanical stability, and good hydrophilicity, which provided a biocompatible microenvironment for maintaining the bioactivity of the immobilized enzyme and prevented the enzyme from leaking out of the film. Therefore, the present biosensor exhibited fast response time (10 s), high sensitivity (12.74 mA cm -2 mM -1 ), long-term operational stability, good suppression of interference, and a wide linear range from 0.02 to 15 mM with a low detection limit of 5 mM for the detection of glucose. In addition, this simple and controllable method could fabricate biosensors in batches with a very small amount of enzyme.

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Section: Condition Optimization For Glucose Sensingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An Amperometric Glucose Biosensor Based on Titania Sol-gel/Prussian Blue Composite Film

2008

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“…10 m 2 /g. [25] CNTs were modified with Prussian Blue using the same procedure already optimized in previous articles [20][21][22] dealing with graphite and glassy carbon, which has brought to high electrochemical activity and good stability of the Prussian Blue layer. In Fig.…”

Section: Characterization Of Pb Modified Carbon Nanotubes Paste Electmentioning

confidence: 99%

Prussian Blue Modified Carbon Nanotube Paste Electrodes: A Comparative Study and a Biochemical Application

et al. 2003

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Prussian Blue modification of Single Walled Carbon Nanotubes and the successive assembling of paste electrodes is presented in this article for the first time. The electrochemical feature of such electrodes have been fully evaluated with CV and amperometric experiments. Prussian Blue Nanotube Paste electrodes showed a high sensitivity towards hydrogen peroxide with a LOD of 7.4 Â 10 À6 M. They also proved to possess a strong stability even at basic pHs (i.e., pH 9 and 10) demonstrating no significant loss of signal after three days of continuous work. In addition, the loading in the paste mixture of glucose oxidase has brought to a sensitive tool for the detection of glucose in a range between 0.1 and 50 mM.

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“…In particular, PB has been recognized as a promising low-potential transducer for H 2 O 2 reduction. The study of the electrocatalytic properties of PB and the development of PB-based chemical or biological sensors have attracted the most attention [3,4]. Nanomaterials exhibit unique optical, electronic and catalytic properties, and have promising applications in various areas [5,6].…”

Section: Introductionmentioning

confidence: 99%

Assembly and electroanalytical performance of Prussian blue/polypyrrole composite nanoparticles synthesized by the reverse micelle method

Miao

Liu

2009

Science and Technology of Advanced Materials

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We report on the characterization, assembly and electroanalytical performance of Prussian blue/polypyrrole (PBPPy) composite nanoparticles synthesized by the reverse micelle method. Scanning electron microscopy suggests the formation of nanosized PBPPy particles with diameters between 40 and 50 nm. Optical absorption confirms that the particles are composed of Prussian blue (PB) and polypyrrole. PB and PBPPy nanoparticles were anchored onto the surface of cysteine-modified Au electrodes. Cyclic voltammetry experiments show that PB-or PBPPy-modified electrodes exhibit intrinsic electrochemical properties and a high electrocatalytic activity towards H 2 O 2 . PBPPy-modified electrodes exhibit a higher sensitivity to H 2 O 2 than PB-modified electrodes. A linear calibration curve in the concentration range 0.99 µM-8.26 mM H 2 O 2 is constructed with a detection limit of 0.23 µM at a signal-to-noise ratio of 3. Excellent stability is observed for PBPPy-composite-nanoparticle-modified electrodes even in a pH 6 phosphate buffer solution with a high H 2 O 2 concentration (0.99 mM). Glutaraldehyde and Nafion R were also employed to immobilize glucose oxidase for the development of PBPPy-based biosensors. The results show that PBPPy composite nanoparticles can be used to develop oxidase-based biosensors.

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Prussian Blue and enzyme bulk-modified screen-printed electrodes for hydrogen peroxide and glucose determination with improved storage and operational stability

Cited by 125 publications

References 25 publications

An Amperometric Glucose Biosensor Based on Titania Sol-gel/Prussian Blue Composite Film

An Amperometric Glucose Biosensor Based on Titania Sol-gel/Prussian Blue Composite Film

Prussian Blue Modified Carbon Nanotube Paste Electrodes: A Comparative Study and a Biochemical Application

Assembly and electroanalytical performance of Prussian blue/polypyrrole composite nanoparticles synthesized by the reverse micelle method

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