PsCYP1 of marine red alga Pyropia seriata (Bangiales, Rhodophyta) confers salt and heat tolerance in Chlamydomonas

High salinity is a challenging environmental stress for organisms to overcome. Unicellular photosynthetic microalgae are especially vulnerable as they have to grapple not only with ionic imbalance and osmotic stress but also with the generated reactive oxygen species (ROS) interfering with photosynthesis. This review attempts to compare and contrast mechanisms that algae, particularly the eukaryotic Chlamydomonas microalgae, exhibit in order to immediately respond to harsh conditions caused by high salinity. The review also collates adaptation mechanisms of freshwater algae strains under persistent high salt conditions. Understanding both short-term and long-term algal responses to high salinity is integral to further fundamental research in algal biology and biotechnology.

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“…Heat and salt tolerance [6] Photosynthetic ferredoxin (PETF) and ferredoxin-5 gene (FDX5) Chlamydomonas sp.…”

Section: Cyclophilins (Cyp) Pscyp1mentioning

confidence: 99%

Salinity Stress Responses and Adaptation Mechanisms in Eukaryotic Green Microalgae

Shetty

Gitau

Maróti

2019

Cells

217

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“…Although previous studies have examined transformed Pyropia species, they involved transiently transformed and unstable algae [16, 17]. Chlamydomonas reinhardtii was recently described as a good vector for the functional verification of Pyropia species genes [16, 17]. Therefore, in the present study, we used C. reinhardtii to comprehensively investigate P. haitanensis desiccation tolerance.…”

Section: Introductionmentioning

confidence: 99%

Insight into transketolase of Pyropia haitanensis under desiccation stress based on integrative analysis of omics and transformation

et al. 2019

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BackgroundPyropia haitanensis, distributes in the intertidal zone, can tolerate water losses exceeding 90%. However, the mechanisms enabling P. haitanensis to survive harsh conditions remain uncharacterized. To elucidate the mechanism underlying P. haitanensis desiccation tolerance, we completed an integrated analysis of its transcriptome and proteome as well as transgenic Chlamydomonas reinhardtii carrying a P. haitanensis gene.ResultsP. haitanensis rapidly adjusted its physiological activities to compensate for water losses up to 60%, after which, photosynthesis, antioxidant systems, chaperones, and cytoskeleton were activated to response to severe desiccation stress. The integrative analysis suggested that transketolase (TKL) was affected by all desiccation treatments. Transgenic C. reinhardtii cells overexpressed PhTKL grew better than the wild-type cells in response to osmotic stress.ConclusionP. haitanensis quickly establishes acclimatory homeostasis regarding its transcriptome and proteome to ensure its thalli can recover after being rehydrated. Additionally, PhTKL is vital for P. haitanensis desiccation tolerance. The present data may provide new insights for the breeding of algae and plants exhibiting enhanced desiccation tolerance.

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“…Recently, studies using transformation have been conducted in Chlamydomonas to characterize the function of the heat shock proteins (HSPs) from Porphyra seriata and Pyropia tenera, [34][35][36]. Similarly, the role of cyclophilins in protein folding, symbiosis, and plant responses to abiotic stress have also been examined following their transformation into Chlamydomonas [37]. Chlamydomonas species are photoautotrophs that have advantages over yeast for elucidating the functional relevance of genes that respond to plant and alga stress [38].…”

Section: Introductionmentioning

confidence: 99%

Study of functional verification to abiotic stress through antioxidant gene transformation of Pyropia yezoensis (Bangiales, Rhodophyta) APX and MnSOD in Chlamydomonas

Lee

Yang

Choi

2018

Journal of Microbiology and Biotechnology

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PsCYP1 of marine red alga Pyropia seriata (Bangiales, Rhodophyta) confers salt and heat tolerance in Chlamydomonas

Cited by 7 publications

References 31 publications

Salinity Stress Responses and Adaptation Mechanisms in Eukaryotic Green Microalgae

Salinity Stress Responses and Adaptation Mechanisms in Eukaryotic Green Microalgae

Insight into transketolase of Pyropia haitanensis under desiccation stress based on integrative analysis of omics and transformation

Study of functional verification to abiotic stress through antioxidant gene transformation of Pyropia yezoensis (Bangiales, Rhodophyta) APX and MnSOD in Chlamydomonas

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