Pseudomonas benzenivorans sp. nov. and Pseudomonas saponiphila sp. nov., Represented by Xenobiotics Degrading Type Strains

Abstract

Two strains of gram-negative bacteria isolated because of their abilities to decompose xenobiotic compounds were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence analysis, the two strains were found to belong to the genus Pseudomonas. Benzene degrading strain DSM 8628(T) was moderately related to P. flavescens NCPP 3063(T) (98.3% similarity), P. monteilii CIP 104883(T), and P. plecoglossicida FPC 951(T) (98.1%). Strain DSM 9751(T) capable to grow with cetyltrimethylammonium chloride as the sole carbon source showed the highest similarity values with P. tremae CFBP 2341(T) and P. meliae MAFF 301463(T) (98.0%), both related to Pseudomonas syringae. The fatty acid pattern of strain DSM 8628(T) was distinct from patterns of other members of the genus Pseudomonas in combining a high ratio of 3OH-C(12:1) (5.1%), a low ratio of 2OH-C(12:0) (0.2%) and a relatively low ratio of C(18:1)omega7c (23.8%). On the basis of phylogenetic analysis, physiological properties and the composition of whole cell fatty acids, two novel species, Pseudomonas benzenivorans sp. nov. with the type strain DSM 8628(T) (=CIP 109857(T)) and Pseudomonas saponiphila sp. nov. with the type strain DSM 9751(T) (=CIP 109856(T)), are proposed.

Pseudomonas hussainii sp. nov., isolated from droppings of a seashore bird, and emended descriptions of Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis

“…The description is as given by Lang et al (2010) with the following amendments. Phosphatidylethanolamine is the predominant polar lipid followed by phosphatidylglycerol.…”

“…Members of the genus Pseudomonas are ubiquitous in distribution, and have been isolated from various sources such as water (Liu et al, 2013;Pascual et al, 2012;Lang et al, 2010;Hauser et al, 2004), soil (Lin et al, 2013a;Feng et al, 2012;Park et al, 2006), seashore sand (Weon et al, 2006) and food-waste compost (Lin et al, 2013b). The nutritional studies of Stanier et al (1966) on Pseudomonas provided baseline assistance to the phenotypic characterization of representative isolates.…”

“…The nutritional studies of Stanier et al (1966) on Pseudomonas provided baseline assistance to the phenotypic characterization of representative isolates. Species of the genus Pseudomonas differ considerably in their nutritional versatility and can metabolize xenobiotic compounds (Lang et al, 2010;Busse et al, 1989). The members of the genus Pseudomonas have undergone multiple taxonomic reassessment on the basis of physiological, molecular and phenotypic features (Sneath et al, 1981), DNA-DNA hybridization (Palleroni, 1984), 16S rRNA gene sequence similarity (Anzai et al, 2000) and chemotaxonomic data (Oyaizu & Komagata, 1983).…”

“…Pale-yellow colonies of strains CC-AMH-11 T and CC-AMHZ-5 were isolated, purified and preserved in nutrient broth (NB; Himedia) supplemented with 20 % (v/v) glycerol at 280 u C. Taxonomic investigations were carried out according to previously published guidelines (Tindall et al, 2010). Three type strains of the genus Pseudomonas [Pseudomonas pohangensis DSM 17875 T (Weon et al, 2006), P. benzenivorans DSM 8628 T (Lang et al, 2010) and P. segetis KCTC 12331 T (Park et al, 2006)] were used for direct comparative analysis. All five strains were cultured by using nutrient agar (NA; Himedia) or NB for 48 h at 30 u C, unless specified otherwise.…”

Pseudomonas kuykendallii sp. nov.: A Novel γ-Proteobacteria Isolated From a Hexazinone Degrading Bioreactor

“…With P. graminis and P. putida the dominant fatty acids were summed feature 3 (C 16:1 x7c and C 16:1 x6c) followed by C 16:0 and then summed feature 8 (C 18:1 x7c/C 18:1 x6c). The detection of large amounts of summed feature 8 (C 18:1 x7c/C 18:1 x6c) in the H2 T strain is similar to strains related to P. aeruginosa and P. stutzeri which often contain large amounts (from 32 up to 40 %) of octadecenoic acid [12]. In addition, the H2 T strain contained significant levels of the hydroxylated acids C 10:0 3OH and C 12:0 3OH, fatty acids that are generally detected in the genus Pseudomonas [12].…”