Pseudomonas plecoglossicida as a novel bacterium for phosphate solubilizing and indole-3-acetic acid-producing from soybean rhizospheric soils of East Java, Indonesia

Abstract

Abstract. Astriani M, Zubaidah S, Abadi AL, Suarsini E. 2020. Pseudomonas plecoglossicida as a novel bacterium for phosphate solubilizing and indole-3-acetic acid-producing from soybean rhizospheric soils of East Java, Indonesia. Biodiversitas 21: 578-586. The use of synthetic fertilizers to grow soybean (Glycine max (L.) Merrill) in a long time, can increase the risk of environmental damage. Therefore, the current study aimed to find phosphate solubilizing bacteria that can produce indole-3-acetic acid (IAA) hormone to minimize the use of chemical fertilizers. Superior isolates selected through characterization of phosphate solubilization activity on Pikovskaya medium, screening of IAA producing bacteria, quantitative estimation of phosphate solubilizing activity using a spectrophotometer, hypersensitivity assay, antagonist within isolate assay, and molecular identification of selected bacterial isolates using 16S rRNA sequencing with primer forward 63f and primer reverse 1387r. Among the isolated bacteria, isolate Arj8 showed the highest phosphate solubilizing activity and IAA production. Molecular identification indicated that isolate Arj8 shared 100% similarity with Pseudomonas plecoglossicida. The highest phosphate solubilizing activity (75.39 mg/L) and IAA production (38.89 ppm) recorded on day-3. Multiple potentialities of P. plecoglossicida as phosphate solubilizing and IAA producing bacterium are a novel finding in the development of bioinoculants as bio-fertilizers that can reduce dependency on synthetic chemical fertilizers.

Phosphate-Solubilizing Microorganisms: Mechanism and Their Role in Phosphate Solubilization and Uptake

“…Reduction in the level of stress abscisic acid as well as an increment in the jasmonic acid and salicylic acid content in the rhizosphere were also observed (Kang et al 2019). Recent advances in the field of biofertilizers have discovered novel elite strains like Pseudomonas plecoglossicida isolated from soybean rhizosphere, which solubilized 75.39 mg L − 1 phosphorus and also produced plant growth hormones like indole acetic acid up to 38.89 ppm (Astriani et al 2020). Gordonia terrae has recently been discovered to solubilize phosphorus up to 299.3 mg L − 1 under highly saline conditions, i.e., 1.5 M NaCl concentration (Jiang et al 2020).…”

Transcriptome Profiling Analysis of Phosphate-Solubilizing Mechanism of Pseudomonas Strain W134

“…Recent advances in the field of biofertilizers have discovered novel elite strains such as Pseudomonas plecoglossicida isolated from soybean rhizosphere, which solubilized 75.39 mg L −1 phosphorus and also produced plant growth hormones such as indole acetic acid up to 38.89 ppm [ 47 ]. The solubility of tricalcium phosphate in W134 was 587.52 mg L −1 ( Table S2 ), which was 679.31% higher than that of Pseudomonas plecoglossicida .…”

Use of phosphorus- and potassium-solubilizing multifunctional microbes to support maize growth and yield

“…Second, PSM and KSM produce phytohormones that stimulate cell elongation at the growing point. According to Astriani et al (2020), PSM produces indole acetic acid, which functions to stimulate cell elongation at the growing point. Viruel et al (2011) also argued that P-solubilizing bacteria have the potential to produce phytohormones and siderophores.…”