Pseudomonas Species as Producers of Eco-friendly Polyhydroxyalkanoates

Abstract

Polyhydroxyalkanoates (PHAs) have been produced by various bacteria as natural polymers stored in bacterial cells as a source of carbon and energy. They are currently preferred biomaterials for use in many industrial fields instead of conventional non-degradable plastics. Due to their unique properties they can reduce pollution caused by the increasing global polymer demand. Pseudomonas species have been chosen as PHAs producers in many recent studies. Being metabolically versatile and possessing a remarkable tolerance to a wide range of carbon sources, these bacteria have become an efficient cell factory for PHAs production. Currently, attention is focused on the design of Pseudomonas strains to increase their ability to accumulate PHAs in the cell and modifying their biosynthetic pathways to obtain strains with modified compositions and improved properties. This article discusses the current state of knowledge of polyhydroxyalkanoates synthesized by Pseudomonas species which are industrially important microorganisms. This review provides an overview of recent trends towards PHA production, focusing on the utilization of low-cost carbon sources, fermentation strategies, PHAs properties and their uses as valuable bioproducts.

Recent Advances in Bioplastics: Application and Biodegradation

“…MclPHAs are used as a source of chiral (R)-3-hydroxyalkanoic acids (R3HAs), which are precursors for the synthesis of value-added chemicals including antibiotics, pharmaceuticals, vitamins [300]. (R)-3-hydroxydecanoic acid obtained via polymer depolymerisation was conjugated to DP18 peptide and its derivatives containing D-amino acids and improved the anti-proliferation activity [31].…”

Production of Polyhydroxyalkanoates and Extracellular Products Using Pseudomonas Corrugata and P. Mediterranea: A Review

“…2 β-galactosidase activities detected by transcriptional fusion plasmids for phaC1, phaC2, and phaI promoter regions based on the pMP220 promoter probe vector and expressed as Miller units. 3 Pairwise comparison of mRNA levels analysis, using the Pme 9.1 sample as a reference (log2 fold change ≥ 2 and p-value ≤ 0.05).…”

“…Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by both Gram-negative and Gram-positive eubacteria, and an increasing number of archaea isolated from environmentally extreme habitats, to increase their survival and competition in environments where carbon and energy sources are limited, such as soil and rhizosphere [1][2][3].…”

“…Their elastic and flexibility properties improve the processability and mechanical properties of blends with other biodegradable polymers [20][21][22]. Of the various species tested worldwide, P. aeruginosa, P. putida, P. resinovorans, P. mendocina, and P. chlororaphis are the most extensively studied to clarify the metabolic processes of the production of PHA and to enhance the bioconversion efficiency [3,23].…”

Engineering the Osmotic State of Pseudomonas putida KT2440 for Efficient Cell Disruption and Downstream Processing of Poly(3-Hydroxyalkanoates)

“…The PHA synthase (PhaC) catalyzes the polymerization process enabling the incorporation of different monomers in the polymeric chain of the polyoxoester, which finally results in distinct thermophysical attributes, thus expanding the number of products and application (Volova et al, 2017;Pacheco et al, 2019). Pseudomonas putida KT2440 is an archetype bacterium for the production of mcl-PHA Mozejko-Ciesielska et al, 2019), where their accumulation in the cell usually occurs under carbon excess accompanied by the limitation of inorganic compounds such as N, O 2 , or P (Prieto et al, 2016).…”