Psychological stress induces alterations in temporomandibular joint ultrastructure in a rat model of temporomandibular disorder

Wu, Gaoyi; Chen, Lei; Zhu, Guoqiang; Su, Yucheng; Chen, Yongjin; Sun, Jin; Wang, Yanliang

doi:10.1016/j.tripleo.2011.06.005

Cited by 17 publications

(22 citation statements)

References 19 publications

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“…Many studies have confirmed that psychological factors such as sleep disorders, psychological stress, and depression may relate to TMJ dysfunction [23]–[25]. Our study reinforces the idea that CSD in rats can indeed cause pathological changes in the TMJ at the molecular level.…”

Section: Discussionsupporting

confidence: 88%

Effects of Chronic Sleep Deprivation on the Extracellular Signal-Regulated Kinase Pathway in the Temporomandibular Joint of Rats

Wang

et al. 2014

PLoS ONE

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ObjectivesTo examine the possible involvement and regulatory mechanisms of extracellular signal-regulated kinase (ERK) pathway in the temporomandibular joint (TMJ) of rats subjected to chronic sleep deprivation (CSD).MethodsRats were subjected to CSD using the modified multiple platform method (MMPM). The serum levels of corticosterone (CORT) and adrenocorticotropic hormone (ACTH) were tested and histomorphology and ultrastructure of the TMJ were observed. The ERK and phospho-ERK (p-ERK) expression levels were detected by Western blot analysis, and the MMP-1, MMP-3, and MMP-13 expression levels were detected by real-time quantitative polymerase chain reaction (PCR) and Western blotting.ResultsThe elevated serum CORT and ACTH levels confirmed that the rats were under CSD stress. Hematoxylin and eosin (HE) staining and scanning electron microscopy (SEM) showed pathological alterations in the TMJ following CSD; furthermore, the p-ERK was activated and the mRNA and protein expression levels of MMP-1, MMP-3, and MMP-13 were upregulated after CSD. In the rats administered with the selective ERK inhibitor U0126, decreased tissue destruction was observed. Phospho-ERK activation was visibly blocked and the MMP-1, MMP-3, and MMP-13 mRNA and protein levels were lower than the corresponding levels in the CSD without U0126 group.ConclusionThese findings indicate that CSD activates the ERK pathway and upregulates the MMP-1, MMP-3, and MMP-13 mRNA and protein levels in the TMJ of rats. Thus, CSD induces ERK pathway activation and causes pathological alterations in the TMJ. ERK may be associated with TMJ destruction by promoting the expression of MMPs.

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Section: Discussionsupporting

confidence: 88%

Effects of Chronic Sleep Deprivation on the Extracellular Signal-Regulated Kinase Pathway in the Temporomandibular Joint of Rats

Wang

et al. 2014

PLoS ONE

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“…Psychological factors, such as sleep disorders, psychological stress, and depression, may cause TMJ dysfunction [19–21]. We demonstrated that sleep disturbance in rats causes significant pathological changes in the TMJ.…”

Section: Discussionmentioning

confidence: 96%

VEGF promotes cartilage angiogenesis by phospho-ERK1/2 activation of Dll4 signaling in temporomandibular joint osteoarthritis caused by chronic sleep disturbance in Wistar rats

Dong

Zhu

et al. 2017

Oncotarget

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Chronic sleep disturbance (CSD) has been linked to the development of temporomandibular joint osteoarthritis (TMJ-OA). While the pathogenesis of TMJ-OA is unclear, recent studies indicate that osteochondral angiogenesis is important. We developed a rat model of CSD induced TMJ-OA to investigate the changes caused by sleep disturbance and to correlate them with vascular invasion in the TMJ. We found pathological alterations and an increased microvessel density in the rat TMJ following CSD. VEGF, Dll4 and p-ERK1/2, the expression of angiogenic factors, were highly expressed in the rat mandibular condylar cartilage and their expression increased with CSD. Furthermore, we show that VEGF-induce activation of ERK1/2, which in turn, increases Dll4 expression. Together, our results suggest that CSD can cause OA-like pathological alterations in the rat TMJ by increasing angiogenesis.

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“…By means of animal stress-inducing models, investigators have shown that psychological factors may play important role in the development of TMD by changes in cortisol levels, TMJ ultra and microstructures observed with electronic microscopy, by increased expression of inflammatory cytokines, substance P and calcitonin levels (CGRP), in addition to increased mioelectric activity of masticatory muscles 32,33 . However, in our sample, emotional wear due to study was not reflected in increased perception of TMD symptoms as years went by.…”

Section: Discussionmentioning

confidence: 99%

Relationship between temporomandibular disorder symptoms signs and Burnout syndrome among dentistry students

Magri¹,

Melchior²,

Jarina³

et al. 2016

Revista Dor

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Psychological stress induces alterations in temporomandibular joint ultrastructure in a rat model of temporomandibular disorder

Cited by 17 publications

References 19 publications

Effects of Chronic Sleep Deprivation on the Extracellular Signal-Regulated Kinase Pathway in the Temporomandibular Joint of Rats

Effects of Chronic Sleep Deprivation on the Extracellular Signal-Regulated Kinase Pathway in the Temporomandibular Joint of Rats

VEGF promotes cartilage angiogenesis by phospho-ERK1/2 activation of Dll4 signaling in temporomandibular joint osteoarthritis caused by chronic sleep disturbance in Wistar rats

Relationship between temporomandibular disorder symptoms signs and Burnout syndrome among dentistry students

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