PTPN12 Affects Nasopharyngeal Carcinoma Cell Proliferation and Migration Through Regulating EGFR

Lin, Qinghai; Wang, Huige; Lin, Xinhua; Zhang, Wenrui; Huang, Sunhua; Zheng, Yandan

doi:10.1089/cbr.2017.2254

Cited by 15 publications

(12 citation statements)

References 25 publications

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“…PTPN12, is a member of the Protein tyrosine phosphatases family (48) and is frequently downregulated in several types of human cancer, including nasopharyngeal carcinoma (28), ovarian cancer (49), breast cancer (50) and hepatocellular carcinoma (51). PTPN12 serves as a tumor-suppressor in cancer initiation and progression by regulating a wide range of biological processes, including cell proliferation, apoptosis, migration, invasion, metastasis and chemotherapeutic resistance (26)(27)(28)(29). The current study demonstrated that PTPN12 upregulation inhibits the proliferation and invasion of RB cells and that the inhibition of miR-503 directly targets PTPN12, suppressing the progression of RB.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA‑503 serves an oncogenic role in retinoblastoma progression by directly targeting PTPN12

Yang

Liu

2019

Exp Ther Med

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Numerous studies have demonstrated that microRNAs (miRNAs or miRs) are abnormally expressed in retinoblastoma (RB). miRNAs may serve a role in oncogene or tumor-suppressor activity in RB genesis and development by modulating various biological processes. miRNAs therefore, may be effective therapeutic targets for miRNA-based therapy in patients with RB. Recently it has been revealed that miR-503 may serve a role in various types of human cancer. However, the expression and functional roles of miR-503 are rarely reported in RB. In the current study, the expression of miR-503 was significantly upregulated in RB tissues and cell lines. In addition, Cell Counting Kit-8 and in vitro invasion assays were performed to assess cell proliferation and invasion, respectively. The results of the present study revealed that miR-503 inhibition impeded RB in vitro cell proliferation and invasion. Furthermore, protein tyrosine phosphatase nonreceptor type 12 (PTPN12) was demonstrated to be a direct target gene of miR-503 in RB cells. PTPN12 overexpression also led to the downregulation of miR-503 in RB cell proliferation and invasion. PTPN12 knockdown could therefore abrogate the effects of miR-503 downregulation in RB cells. In conclusion, the results demonstrated that miR-503 may serve a role in RB oncogenic activity progression by directly targeting PTPN12. Therefore, miR-503 may be a target for effective therapy in patients with RB.

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Section: Discussionmentioning

confidence: 99%

“…3A). PTPN12 has previously been demonstrated to serve as a tumor-suppressant in multiple types of human malignancy, so was selected for additional analysis (26)(27)(28)(29). A luciferase reporter assay was used to verify the prediction that PTPN12 served a role in the expression of miR-503.…”

Section: Ptpn12 Is a Direct Target Gene Of Mir-503 In Rb Cellsmentioning

confidence: 99%

MicroRNA‑503 serves an oncogenic role in retinoblastoma progression by directly targeting PTPN12

Yang

Liu

2019

Exp Ther Med

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“…On the contrary, the overexpression of PTPN12 (protein tyrosine phosphatase nonreceptor type 12) in NPC cells has decreased EGFR expression. It has enhanced caspase3 activity, preventing the proliferation and invasion of tumor cells [ 80 ]. Overall, the EGFR and EGFR signalling pathways play an essential role in the invasion and metastasis of NPC.…”

Section: The Role Of Egfr Pathways In Nasopharyngeal Carcinomamentioning

confidence: 99%

Nasopharyngeal Carcinoma: The Role of the EGFR in Epstein–Barr Virus Infection

et al. 2021

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Epstein–Barr virus (EBV), a type 4 γ herpes virus, is recognized as a causative agent in nasopharyngeal carcinoma (NPC). Incidence of EBV-positive NPC have grown in recent decades along with worse outcomes compared with their EBV-negative counterparts. Latent membrane protein 1 (LMP1), encoded by EBV, induces NPC progression. The epidermal growth factor receptor (EGFR), a member of the ErbB family of receptor tyrosine kinases (RTK), is a driver of tumorigenesis, including for NPC. Little data exist on the relationship between EGFR and EBV-induced NPC. In our initial review, we found that LMP1 promoted the expression of EGFR in NPC in two main ways: the NF-κB pathway and STAT3 activation. On the other hand, EGFR also enhances EBV infection in NPC cells. Moreover, activation of EGFR signalling affects NPC cell proliferation, cell cycle progression, angiogenesis, invasion, and metastasis. Since EGFR promotes tumorigenesis and progression by downstream signalling pathways, causing poor outcomes in NPC patients, EGFR-targeted drugs could be considered a newly developed anti-tumor drug. Here, we summarize the major studies on EBV, EGFR, and LMP1-regulatory EGFR expression and nucleus location in NPC and discuss the clinical efficacy of EGFR-targeted agents in locally advanced NPC (LA NPC) and recurrent or metastatic NPC (R/M NPC) patients.

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“…They serve as templates for protein synthesis on ribosomes and determine the amino acid sequences of peptide chains 15,16 . The expression level and methylation of mRNA are closely related to the processes of tumorigenesis, proliferation, and invasion [17][18][19] . In addition, mRNAs are closely associated with the prognoses of various tumors and can be used as reliable biomarkers for prognosis [20][21][22] .…”

Section: Introductionmentioning

confidence: 99%

Development and Clinical Validation of a Seven-Gene Prognostic Signature Based on Multiple Machine Learning Algorithms in Kidney Cancer

Tian

Wang

2021

Cell Transplant

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About a third of patients with kidney cancer experience recurrence or cancer-related progression. Clinically, kidney cancer prognoses may be quite different, even in patients with kidney cancer at the same clinical stage. Therefore, there is an urgent need to screen for kidney cancer prognosis biomarkers. Differentially expressed genes (DEGs) were identified using kidney cancer RNA sequencing data from the Gene Expression Omnibus (GEO) database. Biomarkers were screened using random forest (RF) and support vector machine (SVM) models, and a multigene signature was constructed using the least absolute shrinkage and selection operator (LASSO) regression analysis. Univariate and multivariate Cox regression analyses were performed to explore the relationships between clinical features and prognosis. Finally, the reliability and clinical applicability of the model were validated, and relationships with biological pathways were identified. Western blots were also performed to evaluate gene expression. A total of 50 DEGs were obtained by intersecting the RF and SVM models. A seven-gene signature (RNASET2, EZH2, FXYD5, KIF18A, NAT8, CDCA7, and WNT7B) was constructed by LASSO regression. Univariate and multivariate Cox regression analyses showed that the seven-gene signature was an independent prognostic factor for kidney cancer. Finally, a predictive nomogram was established in The Cancer Genome Atlas (TCGA) cohort and validated internally. In tumor tissue, RNASET2 and FXYD5 were highly expressed and NAT8 was lowly expressed at the protein and transcription levels. This model could complement the clinicopathological characteristics of kidney cancer and promote the personalized management of patients with kidney cancer.

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PTPN12 Affects Nasopharyngeal Carcinoma Cell Proliferation and Migration Through Regulating EGFR

Abstract: PTPN12 regulates NPC proliferation and migration through negative regulating EGFR. It could be treated as a molecular target for NPC diagnosis and prognosis analysis.

Cited by 15 publications

References 25 publications

MicroRNA‑503 serves an oncogenic role in retinoblastoma progression by directly targeting PTPN12

MicroRNA‑503 serves an oncogenic role in retinoblastoma progression by directly targeting PTPN12

Nasopharyngeal Carcinoma: The Role of the EGFR in Epstein–Barr Virus Infection

Development and Clinical Validation of a Seven-Gene Prognostic Signature Based on Multiple Machine Learning Algorithms in Kidney Cancer

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