Puerarin attenuates the endothelial-mesenchymal transition induced by oxidative stress in human coronary artery endothelial cells through PI3K/AKT pathway

Li, Xuguang; Sun, Shuchan; Chen, Di; Yuan, Tianyi; Chen, Yucai; Wang, Danshu; Fang, Lian-Hua; Lü, Yang; Du, Guanhua

doi:10.1016/j.ejphar.2020.173472

Cited by 20 publications

(12 citation statements)

References 55 publications

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“…In addition to TCM compounds, puerarin, a TCM monomer, could effectively reduce endothelial dysfunction, and protected heart and kidney tissues by inhibiting TGF-β, PI3K, and p38 MAPK signaling, three classic signaling pathways of EndoMT [ 100 , 112 , 113 ]. The TCM monomers astragalus and Paeoniae radix rubra extract can effectively inhibit TGF-β1/Smad2 and 3 signaling pathways and reduce type I and III collagen, fibronectin, and α-SMA levels [114] .…”

Section: Treatment Strategies For Organ Fibrosis Using Western and Tr...mentioning

confidence: 99%

WITHDRAWN: The Role of the Vascular Niche in Organ Fibrosis and COVID-19-Related Organ Damage and the Countermeasures adopted by Chinese and Western Medicine

Zhou

Yang

Sui

et al. 2022

Pharmacological Research - Modern Chinese Medicine

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Section: Treatment Strategies For Organ Fibrosis Using Western and Tr...mentioning

confidence: 99%

WITHDRAWN: The Role of the Vascular Niche in Organ Fibrosis and COVID-19-Related Organ Damage and the Countermeasures adopted by Chinese and Western Medicine

Zhou

Yang

Sui

et al. 2022

Pharmacological Research - Modern Chinese Medicine

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“…In summary, the potential quality markers of XKST were preliminarily identified as danshensu, salvianolic acid B, salvianolic acid A, puerarin, and daidzein. Studies have shown that danshensu, salvianolic acid B, and puerarin have the activity of inhibiting free radical production and antioxidants and play a definite role in the treatment of CVDs ( Lam et al, 2007 ; Wu et al, 2008 ; Zhao et al, 2008 ; Chen et al, 2011 ; Xue et al, 2014 ; Li et al, 2020 ; Wang et al, 2020 ). Meanwhile, danshensu, salvianolic acid B, salvianolic acid A, puerarin, and daidzein all have been noted that they can reduce myocardial injury in a rat ischemia/reperfusion model ( Kim et al, 2009 ; Yin et al, 2013 ; Xue et al, 2014 ; Wang et al, 2020 ; Ling et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

An Integrated Strategy to Identify and Quantify the Quality Markers of Xinkeshu Tablets Based on Spectrum-Effect Relationship, Network Pharmacology, Plasma Pharmacochemistry, and Pharmacodynamics of Zebrafish

et al. 2022

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Xinkeshu tablets (XKST), a traditional Chinese patent medicine (CPM), have served in the clinical treatment of cardiovascular diseases (CVDs) for decades. However, its pharmacodyamic material basis was still unclear, and the holistic quality control has not been well established due to the lack of systematic research on the quality markers. In this experiment, the heart rate recovery rate of a zebrafish larva was used to evaluate the traditional pharmacological effect of XKST i.e., antiarrhythmic effect. The HPLC fingerprints of 16 batches of XKST samples were obtained, and antiarrhythmic components of XKST were identified by establishing the spectrum-effect relationship between HPLC fingerprints and heart rate recovery rate of zebrafish larva with orthogonal signal correction and partial least squares regression (OSC–PLSR) analysis. The anticardiovascular disease components of XKST were identified by mapping the targets related to CVDs in network pharmacology. The compounds of XKST absorbed and exposed in vivo were identified by ultra-high performance liquid chromatography Q-Exactive high-resolution mass spectrometry (UHPLC-Q-Exactive HRMS). Based on the earlier studies, combined with five principles for identifying quality markers and verified by a zebrafish arrhythmia model, danshensu, salvianolic acid A, salvianolic acid B, daidzein, and puerarin were identified as quality markers of XKST. In total, 16 batches of XKST samples were further quantified with the method established in this study. Our study laid the foundation for the quality control of XKST. The integrated strategy used in the study of XKST could be applied for the identification and quantification of quality markers of other CPMs as well.

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“…These studies have con rmed that activating the PI3K/Akt signaling pathway can prevent myocardial ischemia-reperfusion in animal models. Other studies have also suggested that regulation of the PI3K/Akt signaling pathway plays a vital role in inhibiting myocardial brosis, apoptosis, and the in ammatory response [31,32].…”

Section: Discussionmentioning

confidence: 99%

Epithelial-Mesenchymal Transition-Related lncRNAs And SNAI2 Are Potential Biomarkers in Coronary Artery Disease

Zou

Liu

et al. 2021

Preprint

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BackgroundIncreasing evidence suggests that epithelial-mesenchymal transformation (EMT) is critical in the development of inflammatory response, atherosclerosis, and coronary artery disease (CAD). However, landscapes of EMT-related lncRNAs and their target genes have not been fully established in CAD.MethodsLncRNA and mRNA expression profiles obtained from Gene Expression Omnibus (GEO) database were used to identify the differentially expressed mRNAs (DEGs) and lncRNAs (DElncRNAs) between CAD and normal samples. Based on Pearson correlation analysis to identify the EMT-related lncRNAs, the optimal features were identified by receiver operating characteristic (ROC), the least absolute shrinkage and selection operator (LASSO) regression, Support Vector Machine Reverse Feature Elimination (SVM-RFE) algorithms, and logistic regression models were constructed aiming to distinguish CAD from normal samples. The cis and trans-regulatory networks were constructed based on EMT-related lncRNAs. We further estimated the infiltration of the immune cells in CAD patients with the CIBERSORT algorithm, and the correlation between key genes and infiltrating immune cells was analyzed.ResultsIn this study, a logistic regression model with powerful diagnostic capability was constructed based on a total of eight EMT-related lncRNAs identified by two machine learning methods. Then, results of the immune analysis revealed three significant immune cell subsets (CD8 T cells, monocytes, and NK cells) in CAD patients and found EMT-related lncRNAs were closely correlated with these immune cell subsets. By Pearson correlation analysis we got 34 “cis” and “trans” genes. Among them, SNAI2, an EMT-TF gene, was found in the trans-regulatory network of EMT-related lncRNAs. Further, through logistic regression and analysis of immune cell infiltration, we found SNAI2 was a potential biomarker for the diagnosis of CAD but also a close correlation between highly expressed SNAI2 and these three immune cell subsets in CAD patients.ConclusionIn conclusion, these biomarkers have important significance in the diagnosis of CAD patients. Eight EMT-related lncRNAs and SNAI2 can improve our understanding of the molecular mechanism between EMT and CAD.

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Puerarin attenuates the endothelial-mesenchymal transition induced by oxidative stress in human coronary artery endothelial cells through PI3K/AKT pathway

Cited by 20 publications

References 55 publications

WITHDRAWN: The Role of the Vascular Niche in Organ Fibrosis and COVID-19-Related Organ Damage and the Countermeasures adopted by Chinese and Western Medicine

WITHDRAWN: The Role of the Vascular Niche in Organ Fibrosis and COVID-19-Related Organ Damage and the Countermeasures adopted by Chinese and Western Medicine

An Integrated Strategy to Identify and Quantify the Quality Markers of Xinkeshu Tablets Based on Spectrum-Effect Relationship, Network Pharmacology, Plasma Pharmacochemistry, and Pharmacodynamics of Zebrafish

Epithelial-Mesenchymal Transition-Related lncRNAs And SNAI2 Are Potential Biomarkers in Coronary Artery Disease

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