Pulmonary surfactant inhibition of nanoparticle uptake by alveolar epithelial cells

Radiom, Milad; Sarkis, Matthieu; Brookes, Oliver; Oikonomou, Evdokia K.; Baeza‐Squiban, Armelle; Berret, Jean‐François

doi:10.1038/s41598-020-76332-7

Cited by 36 publications

(21 citation statements)

References 49 publications

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“…Different studies have demonstrated that surfactant components interact with nanoparticles. These interactions can determine the lifetime, fate, and toxicity of the nanomaterial in the airways [9,37] as well as adversely affect pulmonary surfactant function [6]. Therefore, in this work, we evaluated whether PHA nanoparticles are suitable for lung drug delivery by studying the interaction of this nanomaterial with pulmonary surfactant and interfacial films made of the main surfactant lipids.…”

Section: Discussionmentioning

confidence: 99%

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Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant

et al. 2021

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Polyhydroxyalkanoates (PHA) are polyesters produced intracellularly by many bacterial species as energy storage materials, which are used in biomedical applications, including drug delivery systems, due to their biocompatibility and biodegradability. In this study, we evaluated the potential application of this nanomaterial as a basis of inhaled drug delivery systems. To that end, we assessed the possible interaction between PHA nanoparticles (NPs) and pulmonary surfactant using dynamic light scattering, Langmuir balances, and epifluorescence microscopy. Our results demonstrate that NPs deposited onto preformed monolayers of DPPC or DPPC/POPG bind these surfactant lipids. This interaction facilitated the translocation of the nanomaterial towards the aqueous subphase, with the subsequent loss of lipid from the interface. NPs that remained at the interface associated with liquid expanded (LE)/tilted condensed (TC) phase boundaries, decreasing the size of condensed domains and promoting the intermixing of TC and LE phases at submicroscopic scale. This provided the stability necessary for attaining high surface pressures upon compression, countering the destabilization induced by lipid loss. These effects were observed only for high NP loads, suggesting a limit for the use of these NPs in pulmonary drug delivery.

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Section: Discussionmentioning

confidence: 99%

“…[6]). On the other hand, the formation of the corona alters the surface properties of the nanoparticles and, therefore, can modify their biodistribution and toxicity [8,9]. For instance, the coating of nanoparticulated gels with a lipoprotein film that contains SP-B favors the vehiculization of encapsulated small interfering RNA [10,11].…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant

et al. 2021

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“…In contrast, if AgNPs avoid deposition by the conciliary escalator, they will reach the alveoli, where a protective 0.2-5 µM layer of pulmonary surfactant can bind to the AgNPs' surface by corona formation processes leading to excretion, ingestion, or translocation. The fluid secreted by type II cells is composed of lipids and hydrophobic surfactant proteins [119]. The last line of defense contains type I and II pneumocytes monolayer in the epithelial tissue of the alveoli and a group of heterogeneous macrophages distributed on the respiratory surface.…”

Section: Nanotoxicity Models To Evaluate Lung Cytotoxicitymentioning

confidence: 99%

Lung Models to Evaluate Silver Nanoparticles’ Toxicity and Their Impact on Human Health

et al. 2022

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Nanomaterials (NMs) solve specific problems with remarkable results in several industrial and scientific areas. Among NMs, silver nanoparticles (AgNPs) have been extensively employed as drug carriers, medical diagnostics, energy harvesting devices, sensors, lubricants, and bioremediation. Notably, they have shown excellent antimicrobial, anticancer, and antiviral properties in the biomedical field. The literature analysis shows a selective cytotoxic effect on cancer cells compared to healthy cells, making its potential application in cancer treatment evident, increasing the need to study the potential risk of their use to environmental and human health. A large battery of toxicity models, both in vitro and in vivo, have been established to predict the harmful effects of incorporating AgNPs in these numerous areas or those produced due to involuntary exposure. However, these models often report contradictory results due to their lack of standardization, generating controversy and slowing the advances in nanotoxicology research, fundamentally by generalizing the biological response produced by the AgNP formulations. This review summarizes the last ten years’ reports concerning AgNPs’ toxicity in cellular respiratory system models (e.g., mono-culture models, co-cultures, 3D cultures, ex vivo and in vivo). In turn, more complex cellular models represent in a better way the physical and chemical barriers of the body; however, results should be used carefully so as not to be misleading. The main objective of this work is to highlight current models with the highest physiological relevance, identifying the opportunity areas of lung nanotoxicology and contributing to the establishment and strengthening of specific regulations regarding health and the environment.

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“…[ 40 ] In the alveolar epithelium, pulmonary surfactant presents a barrier to NP uptake. [ 41 ] The pulmonary surfactant is composed of 92% lipids (consisting predominantly of zwitterionic and dipalmitoylphosphatidylcholines) and protein [ 42 ] and forms a 0.2–0.5 µm thick layer on the surface of epithelium cells. The proteins, notably surfactant proteins SP‐A and SP‐D, can mediate interactions with immune cells and facilitate phagocytosis presenting a barrier that can be avoided or exploited to achieve effective drug delivery.…”

Section: Single Cell Levelmentioning

confidence: 99%

A Focus on “Bio” in Bio–Nanoscience: The Impact of Biological Factors on Nanomaterial Interactions

Cortez‐Jugo

Czuba-Wojnilowicz

Tan

et al. 2021

Adv Healthcare Materials

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Bio–nanoscience research encompasses studies on the interactions of nanomaterials with biological structures or what is commonly referred to as the biointerface. Fundamental studies on the influence of nanomaterial properties, including size, shape, composition, and charge, on the interaction with the biointerface have been central in bio–nanoscience to assess nanomaterial efficacy and safety for a range of biomedical applications. However, the state of the cells, tissues, or biological models can also influence the behavior of nanomaterials at the biointerface and their intracellular processing. Focusing on the “bio” in bio–nano, this review discusses the impact of biological properties at the cellular, tissue, and whole organism level that influences nanomaterial behavior, including cell type, cell cycle, tumor physiology, and disease states. Understanding how the biological factors can be addressed or exploited to enhance nanomaterial accumulation and uptake can guide the design of better and suitable models to improve the outcomes of materials in nanomedicine.

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Pulmonary surfactant inhibition of nanoparticle uptake by alveolar epithelial cells

Cited by 36 publications

References 49 publications

Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant

Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant

Lung Models to Evaluate Silver Nanoparticles’ Toxicity and Their Impact on Human Health

A Focus on “Bio” in Bio–Nanoscience: The Impact of Biological Factors on Nanomaterial Interactions

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