Pulmonary toxoplasmosis in immunocompromised patients with interstitial pneumonia: a single-centre prospective study assessing PCR-based diagnosis

Désoubeaux, Guillaume; Cabanne, Eglantine; Franck-Martel, Claire; Gombert, Martin; Gyan, Emmanuel; Lissandre, Séverine; Renaud, Marc; Monjanel, Hélène; Dartigeas, Caroline; Bailly, Éric; Langendonck, Nathalie Van; Chandenier, J.

doi:10.1136/jclinpath-2015-203385

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…Marked vascular dilatation and congestion were seen in all hepatic vessels, including central and portal veins as well as sinusoids. Infected humans and naturally occurring animal cases of pulmonary toxoplasmosis have also been documented [ 68 , 69 ]. In the present work, histopathological examination of liver and lung tissues of all studied groups aimed to verify the results of parasitological and molecular analyses.…”

Section: Discussionmentioning

confidence: 99%

Wheat Germ Oil and Propolis Decrease Parasite Burden and Restore Marked Histopathological Changes in Liver and Lung in Mice with Chronic Toxoplasmosis

Barakat

Fadaly

Gareh

et al. 2022

Animals

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Toxoplasmosis is a parasitic zoonotic disease with a worldwide distribution. Its effects can be critical in immunocompromised patients. However, there is a limited availability of effective, low-toxicity drugs against this disease, particularly in its chronic form. The present study evaluated the effect of propolis and wheat germ oil (WGO) as safe, natural products to reduce Toxoplasma cysts in experimentally infected mice. For the experiment, five groups (10 mice per group) were examined: Group 1: negative control (noninfected, nontreated); Group 2: positive control (infected, nontreated); Group 3: infected and treated with WGO at a dose of 0.2 mg/1.5 mL per kg body weight/day; Group 4: infected and treated with 0.1 mL propolis extract/day; and Group 5: infected and treated with a combination of WGO and propolis at the same doses as Group 3 and 4. After the mice were sacrificed, liver and lung specimens underwent histopathological examination, and the parasite burden was investigated by parasitological methods and quantified using real-time polymerase chain reaction. Notably, the results showed a substantial decrease in parasitic burden in Group 5 compared to the control group. These results were further confirmed by molecular analysis and quantification of the DNA concentration of the Toxoplasma P29 gene after treatment in all tested samples. Furthermore, the combination of propolis and WGO restored all histopathological changes in the liver and lungs. Taken together, these findings provide remarkably promising evidence of the effects of the combination of WGO and propolis against chronic toxoplasmosis in mice.

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Section: Discussionmentioning

confidence: 99%

Wheat Germ Oil and Propolis Decrease Parasite Burden and Restore Marked Histopathological Changes in Liver and Lung in Mice with Chronic Toxoplasmosis

Barakat

Fadaly

Gareh

et al. 2022

Animals

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“…Pulmonary toxoplasmosis has been previously reported in naturally infected animals and in human cases [ 57 , 58 , 59 , 60 ]. In the present study, we examined the lung tissue of all animal groups to evaluate the therapeutic effect of ginger against pulmonary toxoplasmosis.…”

Section: Discussionmentioning

confidence: 99%

Ginger Is a Potential Therapeutic for Chronic Toxoplasmosis

et al. 2022

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Background:Toxoplasma gondii (T. gondii) is an opportunistic parasite that causes serious diseases in humans, particularly immunocompromised individuals and pregnant women. To date, there are limited numbers of therapeutics for chronic toxoplasmosis which necessitate the discovery of effective and safe therapeutics. In the present study, we aimed to evaluate the antitoxoplasmosis potential of ginger extract in mice with experimentally induced chronic toxoplasmosis. Results: Treatment with ginger extract significantly reduced cysts count in the brains of T. gondii-infected mice with a marked alleviation of edema and inflammation, and a reversal of neuronal injury. Moreover, ginger extract treatment reduced inflammation in liver and lungs and protected hepatocytes from infection-induced degeneration. Consistently, apoptosis was significantly mitigated in the brains of ginger extract-treated mice compared to infected untreated animals or spiramycin-treated animals. Methods: Four groups of Swiss albino mice (10 mice each) were used. The first group was not infected, whereas 3 groups were infected with Me49 T. gondii strains. One infected group remained untreated (infected untreated), whereas the other two infected groups were treated with either ginger extract (250 mg/kg) or spiramycin (positive control; 100 mg/kg), respectively. The therapeutic potential of ginger extract was evaluated by calculation of the parasite burden in infected animals, and examination of the infected tissues for reduced pathologic changes. Conclusions: Our results showed for the first time that ginger extract exhibited marked therapeutic effects in mice with chronic T. gondii infection which indicates that it can be used as a safe and effective treatment for chronic toxoplasmosis.

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“…La serología sigue siendo una de las principales ayudas diagnósticas [7], tal como ocurrió en el paciente reportado, quien tuvo IgM e IgG positivas, lo que permitió con rmar el diagnostico sin realuzar prueba RT-PCR para T. gondii, e instaurar un tratamiento para evitar progresión y complicaciones.…”

Section: Discussionunclassified

“…Aunque en la mayoría de los casos la toxoplasmosis es asintomática, esta es una infección oportunista potencialmente mortal en pacientes inmunocomprometidos de todas las edades. Similar a la neumonía por Pneumocystis, la toxoplasmosis se diagnostica con mayor frecuencia en pacientes con VIH que reciben terapia inmunosupresora [6,7]; sin embargo, los pacientes con trasplantes también son una población de alto riesgo para contraer esta enfermedad.…”

Section: Introductionunclassified

“…En los receptores de trasplante, tanto de órgano sólido como de células madre hematopoyéticas, la toxoplasmosis puede ser el resultado de la reactivación de una infección latente pasada o de una infección primaria adquirida a través de alimentos contaminados o de un órgano trasplantado que contiene quistes latentes [8]. Contrario alo que sucede con la incidencia en pacientes infectados por el VIH, la incidencia de toxoplasmosis entre los receptores de trasplantes está poco documentada y los estudios publicados que informan series de pacientes son escasos [9][10][11], además la literatura consiste principalmente en reportes de casos.…”

Section: Introductionunclassified

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Toxoplasmosis cerebral luego de un trasplante renal

Cabarcas

Pérez

Quiroz

et al. 2021

Rev. Colomb. Nefrol.

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Se reporta el caso de un paciente masculino de 29 años con antecedente de diabetes mellitus tipo 1, con enfermedad renal crónica estadio 5 secundaria a enfermedad renal diabética que requirió de trasplante renal de donante vivo en el año 2018 y en tratamiento inmunosupresor con prednisolona, tacrolimus y micofenolato desde dicho procedimiento, quien ingresó al servicio de urgencias por crisis epiléptica focal con generalización secundaria. El enfoque diagnóstico de toxoplasmosis cerebral en pacientes inmunocomprometidos es un reto clínico debido a la gran variabilidad de signos y síntomas asociados en esta población y a la alta frecuencia de complicaciones, lo cual puede confundir el diagnostico.

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Pulmonary toxoplasmosis in immunocompromised patients with interstitial pneumonia: a single-centre prospective study assessing PCR-based diagnosis

Abstract: 2015_030, May 27th 2015.

Cited by 13 publications

References 31 publications

Wheat Germ Oil and Propolis Decrease Parasite Burden and Restore Marked Histopathological Changes in Liver and Lung in Mice with Chronic Toxoplasmosis

Wheat Germ Oil and Propolis Decrease Parasite Burden and Restore Marked Histopathological Changes in Liver and Lung in Mice with Chronic Toxoplasmosis

Ginger Is a Potential Therapeutic for Chronic Toxoplasmosis

Toxoplasmosis cerebral luego de un trasplante renal

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