Pulsed amperometric detection of glucose in biological fluids at a surface-modified gold electrode

Bindra, Dilbir S.; Wilson, George S.

doi:10.1021/ac00197a022

Cited by 87 publications

(36 citation statements)

References 30 publications

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“…A coating of Nafion has been successfully used to eliminate the interference of AA in the determination of glucose. 27 After a redox polymer/enzyme film was further coated with Nafion film, cyclic voltammograms of Nafion-coated redox polymer/enzyme film was similar to that of the redox polymer/enzyme film. The amperometric response of glucose at the Nafion-coated redox polymer/enzyme film allowed little changes, indicating that the existence of Nafion film had little effect on the electrochemical characteristics of the redox polymer/enzyme film.…”

Section: Elimination Of the Interferences Of Aamentioning

confidence: 88%

An Amperometric Biosensor for Glucose Based on Electrodeposited Redox Polymer/Glucose Oxidase Film on a Gold Electrode

Fei

et al. 2003

ANAL. SCI.

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Since Clark and Lyons developed the first enzyme-based oxygen electrode, 1 enzyme-based biosensors have been used in an increasing number of clinical, environmental, agriculture, and biotechnological applications. [2][3][4][5] In response to the needs for frequent or continuous monitoring of glucose in diabetics, particularly in brittle diabetics, glucose sensors are, by far, the most widely employed, and therefore continue to drive research toward better sensors. [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] Since the redox centers (FAD/FADH2) of glucose oxidase (GOX) are prevented from transferring electrons to an electrode surface by an insulating glycoprotein shell, 6 the presence of a mediator is necessary to achieve direct electron-exchange between the electrode and the redox center of GOX. The pioneering work of Heller 7,8 demonstrated that it is possible to modify GOX with ferrocene derivatives and ruthenium pentaamimine, thus promoting relayed electron-transfer to the electrode.A further experimental and theoretical study 9 revealed that the rates of intramolecular electron-transfer are very sensitive to the particular ferrocene derivative employed, and also the distance between the flavin ring and the point of covalent attachment of the mediator. It is not necessary to have a large number of mediator molecules attached; they need instead to be in the "right" locations. Terathiafulvalence (TIF) and its derivatives are another group of redox molecules that can mediate the direct oxidation of GOX.10-12 However, hydrophobically incorporated TIF is slowly lost from the enzyme, presumably due to its slight solubility in aqueous solution and its enhanced solubility in the oxidized (TIF + ) form, which ultimately limits the use of this approach.Electrically "wired" enzymes by redox hydrogels were introduced by Heller's group, [13][14][15] and provided three-dimension electronic conductivity between enzymes and electrodes. Redox hydrogel films are unique in having adequate electron diffusion coefficients and in being permeable to water-soluble substrates and products of enzymatic reactions. 16 When a crosslinked redox polymer network electrically "wired" an enzyme covalently bound to it, the gel and the substrate electrode formed an enzyme electrode. Unlike enzyme electrodes based on diffusional mediators, they are potentially useful in a flow system and in vivo determination. Heller's group carried out a series of studies, [17][18][19][20][21][22][23] and employed the redox hydrogels for sensors implanted in the subcutaneous tissue of rats 19 and in the vascular bed. 23 It is reported that upon illumination, the redox polymer formed by coordinating [Os(bpy)2Cl + /Os(bpy)2Cl] 2+ to poly(4-vinyl-pyridine) exchanges its inner-sphere chloride with more strongly coordinating pyridine or imidazole groups on the polymer backbones. 24 The resulting films conduct electrons when they are hydrated, and their redox segments are mobile enough to collide, even though they are tethered to the crosslinked ...

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Section: Elimination Of the Interferences Of Aamentioning

confidence: 88%

An Amperometric Biosensor for Glucose Based on Electrodeposited Redox Polymer/Glucose Oxidase Film on a Gold Electrode

Fei

et al. 2003

ANAL. SCI.

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“…20 However, Nafion, together with cellulose acetate, was employed as an inner film in order to eliminate the influence of electroactive compounds existing in biological fluids, such as ascorbic acid, uric acid and acetaminophen. Considering that the utmost employment of low environmental load materials will be a higher requirement from now on, it is advisable that the use of PFCs should be reduced by possible means.…”

Section: Notesmentioning

confidence: 99%

“…The obtained electrode is referred to herein as ''GOx/PGA-CA''. The preparation of a GOx-immobilized electrode with Nafion inner film was carried out according to methods of Wilson et al 19,20 That is, firstly, inner films were prepared by alternate five-times coatings of both Nafion and cellulose acetate using a 5% Nafion solution and a 5% cellulose acetate solution, respectively. Secondly, a mixture solution of bovine serum albumin, GOx, and glutaldehyde was poured on the inner film-coated electrode surface.…”

Section: Preparation Of a Fine Needle-type Glucose Sensormentioning

confidence: 99%

“…[16][17][18][19] Moreover, perfluorinated-sulfonated ion-exchange polymer "Nafion ® " is another popularly used material in the fabrication of biosensors, since it is known to be effective for eliminating the influence of electroactive compounds on the sensor response by an electrostatic interaction. [19][20][21][22][23][24][25][26][27][28] Recently, a low-cost non-animal origin biodegradable polyamino acid, γ-polyglutamic acid (PGA), has received attention for clinical applications due to its good biocompatibility. 29 We previously reported that PGA can be employed as an enzyme immobilizing material for the preparation of a fine needle-type glucose sensor.…”

Section: Introductionmentioning

confidence: 99%

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Highly Selective Needle-Type Glucose Sensors Prepared by the Immobilization of Glucose Oxidase on γ-Polyglutamic Acid Film

et al. 2011

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“…20 Moreover, glucose Integration of a Nanoporous Platinum Thin Film into a Microfluidic System for Non-enzymatic Electrochemical Glucose Sensing sensors based on nanoporous Pt technology are compatible with conventional microfabrication post-processing, since the electrode is composed of Pt. In addition, unlike pulsed amperometric detection, 21,22 nanoporous Pt does not require a sample separation step, because its selectivity prevents REDOX interference by species like ascorbic acid or acetaminophenol. Therefore, it is conceivable that a combination of nanoporous Pt with an appropriate microfluidic system handling sample solutions in a programmed sequence act as a nonenzymatic glucose sensor with useful additional functions, such as, automatic calibration, high-throughput analytical power, and reusability.…”

Section: Introductionmentioning

confidence: 99%

Integration of a Nanoporous Platinum Thin Film into a Microfluidic System for Non-enzymatic Electrochemical Glucose Sensing

et al. 2007

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In this paper, we describe an amperometric-type enzymeless glucose sensing system based on a nanoporous platinum (Pt) electrode embedded in a microfluidic chip. This microchip system is comprised of a microfluidic transport channel network and a miniaturized electrochemical cell for nonenzymatic glucose sensing. Sample and buffer solutions were transferred to the cell by programmed electroosmotic flow (EOF). A nanoporous Pt electrode with the roughness factor of 200.6 was utilized to determine glucose concentrations in phosphate buffered saline (PBS) by the direct oxidation of glucose, without any separation process. The sensitivity of the developed system is 1.65 μA cm -2 mM -1 in the glucose concentration range from 1 -10 mM in PBS.

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Pulsed amperometric detection of glucose in biological fluids at a surface-modified gold electrode

Cited by 87 publications

References 30 publications

An Amperometric Biosensor for Glucose Based on Electrodeposited Redox Polymer/Glucose Oxidase Film on a Gold Electrode

An Amperometric Biosensor for Glucose Based on Electrodeposited Redox Polymer/Glucose Oxidase Film on a Gold Electrode

Highly Selective Needle-Type Glucose Sensors Prepared by the Immobilization of Glucose Oxidase on γ-Polyglutamic Acid Film

Integration of a Nanoporous Platinum Thin Film into a Microfluidic System for Non-enzymatic Electrochemical Glucose Sensing

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